Investigating the Impact of Multi-Heme Pyrroloquinoline Quinone-Aldehyde Dehydrogenase Orientation on Direct Bioelectrocatalysis via Site Specific Enzyme Immobilization

ACS Catalysis ◽  
2013 ◽  
Vol 3 (8) ◽  
pp. 1756-1763 ◽  
Author(s):  
Shuai Xu ◽  
Shelley D. Minteer
Keyword(s):  
Enzyme Immobilization ◽  
Aldehyde Dehydrogenase ◽  
Pyrroloquinoline Quinone ◽  
Specific Enzyme ◽  
Site Specific ◽  
The Impact

scholarly journals Engineered catalytic biofilms: Site-specific enzyme immobilization ontoE. colicurli nanofibers

2015 ◽  
Vol 112 (10) ◽  
pp. 2016-2024 ◽  
Author(s):  
Zsofia Botyanszki ◽  
Pei Kun R. Tay ◽  
Peter Q. Nguyen ◽  
Martin G. Nussbaumer ◽  
Neel S. Joshi
Keyword(s):  
Enzyme Immobilization ◽  
Specific Enzyme ◽  
Site Specific

Design of a Specific Peptide Tag that Affords Covalent and Site-Specific Enzyme Immobilization Catalyzed by Microbial Transglutaminase

2005 ◽  
Vol 6 (4) ◽  
pp. 2299-2304 ◽  
Author(s):  
Jo Tominaga ◽  
Noriho Kamiya ◽  
Satoshi Doi ◽  
Hirofumi Ichinose ◽  
Tatsuo Maruyama ◽  
...  
Keyword(s):  
Enzyme Immobilization ◽  
Microbial Transglutaminase ◽  
Specific Enzyme ◽  
Site Specific ◽  
Specific Peptide ◽  
Peptide Tag

scholarly journals Molecular Decoration of Ceramic Supports for Highly Effective Enzyme Immobilization—Material Approach

Materials ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 201
Author(s):  
Joanna Kujawa ◽  
Marta Głodek ◽  
Izabela Koter ◽  
Borys Ośmiałowski ◽  
Katarzyna Knozowska ◽  
...  
Keyword(s):  
Enzyme Immobilization ◽  
Immobilized Lipase ◽  
Material Surface ◽  
Specific Enzyme ◽  
Enzyme Loading ◽  
Enantiomeric Ratio ◽  
Highly Effective ◽  
Functionalized Materials ◽  
Specific Material ◽  
The Impact

A highly effective method was developed to functionalize ceramic supports (Al2O3 powders and membranes) using newly synthesized spacer molecules. The functionalized materials were subsequently utilized for Candida antarctica lipase B enzyme immobilization. The objective is to systematically evaluate the impact of various spacer molecules grafted onto the alumina materials will affect both the immobilization of the enzymes and specific material surface properties, critical to enzymatic reactors performance. The enzyme loading was significantly improved for the supports modified with shorter spacer molecules, which possessed higher grafting effectiveness on the order of 90%. The specific enzyme activity was found to be much higher for samples functionalized with longer modifiers yielding excellent enantioselectivity >97%. However, the enantiomeric ratio of the immobilized lipase was slightly lower in the case of shorter spacer molecules.

The Impact of Bioconjugation on the Interfacial Activity of a Protein Biosurfactant

2018 ◽  
Author(s):  
Hossam H Tayeb ◽  
Marina Stienecker ◽  
Anton Middelberg ◽  
Frank Sainsbury
Keyword(s):  
Polyethylene Glycol ◽  
Colloidal Stability ◽  
Point Mutations ◽  
Pharmaceutical Formulations ◽  
Industrial Processes ◽  
Parent Molecule ◽  
Site Specific ◽  
Interfacial Activity ◽  
Surface Active ◽  
The Impact

Biosurfactants, are surface active molecules that can be produced by renewable, industrially scalable biologic processes. DAMP4, a designer biosurfactant, enables the modification of interfaces via genetic or chemical fusion to functional moieties. However, bioconjugation of addressable amines introduces heterogeneity that limits the precision of functionalization as well as the resolution of interfacial characterization. Here we designed DAMP4 variants with cysteine point mutations to allow for site-specific bioconjugation. The DAMP4 variants were shown to retain the structural stability and interfacial activity characteristic of the parent molecule, while permitting efficient and specific conjugation of polyethylene glycol (PEG). PEGylation results in a considerable reduction on the interfacial activity of both single and double mutants. Comparison of conjugates with one or two conjugation sites shows that both the number of conjugates as well as the mass of conjugated material impacts the interfacial activity of DAMP4. As a result, the ability of DAMP4 variants with multiple PEG conjugates to impart colloidal stability on peptide-stabilized emulsions is reduced. We suggest that this is due to constraints on the structure of amphiphilic helices at the interface. Specific and efficient bioconjugation permits the exploration and investigation of the interfacial properties of designer protein biosurfactants with molecular precision. Our findings should therefore inform the design and modification of biosurfactants for their increasing use in industrial processes, and nutritional and pharmaceutical formulations.

scholarly journals Site-specific N-glycosylation analysis of animal cell culture-derived Zika virus proteins

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Alexander Pralow ◽  
Alexander Nikolay ◽  
Arnaud Leon ◽  
Yvonne Genzel ◽  
Erdmann Rapp ◽  
...  
Keyword(s):  
Zika Virus ◽  
Animal Cell ◽  
Gradient Centrifugation ◽  
Viral Envelope ◽  
Protein Glycosylation ◽  
High Yield ◽  
Structural Protein ◽  
Site Specific ◽  
E Protein ◽  
The Impact

AbstractHere, we present for the first time, a site-specific N-glycosylation analysis of proteins from a Brazilian Zika virus (ZIKV) strain. The virus was propagated with high yield in an embryo-derived stem cell line (EB66, Valneva SE), and concentrated by g-force step-gradient centrifugation. Subsequently, the sample was proteolytically digested with different enzymes, measured via a LC–MS/MS-based workflow, and analyzed in a semi-automated way using the in-house developed glyXtoolMS software. The viral non-structural protein 1 (NS1) was glycosylated exclusively with high-mannose structures on both potential N-glycosylation sites. In case of the viral envelope (E) protein, no specific N-glycans could be identified with this method. Nevertheless, N-glycosylation could be proved by enzymatic de-N-glycosylation with PNGase F, resulting in a strong MS-signal of the former glycopeptide with deamidated asparagine at the potential N-glycosylation site N444. This confirmed that this site of the ZIKV E protein is highly N-glycosylated but with very high micro-heterogeneity. Our study clearly demonstrates the progress made towards site-specific N-glycosylation analysis of viral proteins, i.e. for Brazilian ZIKV. It allows to better characterize viral isolates, and to monitor glycosylation of major antigens. The method established can be applied for detailed studies regarding the impact of protein glycosylation on antigenicity and human pathogenicity of many viruses including influenza virus, HIV and corona virus.

scholarly journals The influence of riparian woodland on the spatial and temporal variability of stream water temperatures in an upland salmon stream

2004 ◽  
Vol 8 (3) ◽  
pp. 449-459 ◽  
Author(s):  
I. A. Malcolm ◽  
D. M. Hannah ◽  
M. J. Donaghy ◽  
C. Soulsby ◽  
A. F. Youngson
Keyword(s):  
Thermal Regime ◽  
Temporal Variability ◽  
Heat Budget ◽  
Riparian Forest ◽  
Stream Water ◽  
Spatial And Temporal Variability ◽  
Substantial Impact ◽  
Site Specific ◽  
The Impact ◽  
Riparian Woodland

Abstract. The spatio-temporal variability of stream water temperatures was investigated at six locations on the Girnock Burn (30km2 catchment), Cairngorms, Scotland over three hydrological years between 1998 and 2002. The key site-specific factors affecting the hydrology and climatology of the sampling points were investigated as a basis for physical process inference. Particular emphasis was placed on assessing the effects of riparian forest in the lower catchment versus the heather moorland riparian zones that are spatially dominant in the upper catchment. The findings were related to river heat budget studies that provided process detail. Gross changes in stream temperature were affected by the annual cycle of incoming solar radiation and seasonal changes in hydrological and climatological conditions. Inter-annual variation in these controlling variables resulted in inter-annual variability in thermal regime. However, more subtle inter-site differences reflected the impact of site-specific characteristics on various components of the river energy budget. Inter-site variability was most apparent at shorter time scales, during the summer months and for higher stream temperatures. Riparian woodland in the lower catchment had a substantial impact on thermal regime, reducing diel variability (over a period of 24 hours) and temperature extremes. Observed inter-site differences are likely to have a substantial effect on freshwater ecology in general and salmonid fish in particular. Keywords: temperature, thermal regime, forest, salmon, hydrology, Girnock Burn, Cairngorm

scholarly journals Assembly: Performing the materiality of Muslim prayer spaces

Scene ◽  
2018 ◽  
Vol 6 (2) ◽  
pp. 133-151 ◽  
Author(s):  
Julie Marsh
Keyword(s):  
Social Boundaries ◽  
Research Project ◽  
Site Specific ◽  
Specific Research ◽  
A Site ◽  
The Impact

This article reflects on the significance and impact of Assembly, a site-specific research project made and exhibited in Birmingham Central Mosque, Brick Lane Mosque and Old Kent Road Mosque from 2016 to 2020. Assembly provided an opportunity for Muslims and non-Muslims to experience Jumu’ah prayer first-hand via the site performances, which temporarily dissolved the religious/social boundaries of each mosque. Each performance highlighted the differences and relations between each site, furthering ideas of performativity in Muslim prayer spaces. This article summarizes the impact reported by each mosque community as well as reflecting upon the relationships built within the wider community.

scholarly journals WATER VAPOUR WEIGHTED MEAN TEMPERATURE MODEL FOR GPS-DERIVED INTEGRATED WATER VAPOUR IN PENINSULAR MALAYSIA

2017 ◽  
Vol XLII-4/W5 ◽  
pp. 127-135 ◽  
Author(s):  
T. A. Musa ◽  
M. H. Mazlan ◽  
Y. D. Opaluwa ◽  
I. A. Musliman ◽  
Z. M. Radzi
Keyword(s):  
Water Vapour ◽  
Diurnal Cycle ◽  
Specific Model ◽  
Regional Model ◽  
Peninsular Malaysia ◽  
Site Specific ◽  
Weighted Mean Temperature ◽  
Integrated Water Vapour ◽  
Relationship Of ◽  
The Impact

This paper presents the development of T<sub>M</sub> model by using the radiosonde stations from Peninsular Malaysia. Two types of T<sub>M</sub> model were developed; site-specific and regional models. The result revealed that the estimation from site-specific model has small improvement compared to the regional model, indicating that the regional model is adequately to use in estimation of GPS-derived IWV over Peninsular Malaysia. Meanwhile, this study found that the diurnal cycle of T<sub>S</sub> has influenced the T<sub>M</sub>&amp;ndash;T<sub>S</sub> relationship. The separation between daytime and nighttime observation can improve the relationship of T<sub>M</sub>&amp;ndash;T<sub>S</sub>. However, the impact of diurnal cycle to IWV estimation is less than 1&amp;thinsp;%. The T<sub>M</sub> model from Global and Tropic also been evaluated. The Tropic T<sub>M</sub> model is superior to be utilized as compared to the Global T<sub>M</sub> model.

scholarly journals Computational Modeling of the Human Microbiome

2020 ◽  
Vol 8 (2) ◽  
pp. 197
Author(s):  
Shomeek Chowdhury ◽  
Stephen S. Fong
Keyword(s):  
Computational Modeling ◽  
Human Health ◽  
Large Scale ◽  
Human Microbiome ◽  
Human Microbiome Project ◽  
Microbial Composition ◽  
Site Specific ◽  
Microbiome Research ◽  
High Level ◽  
The Impact

The impact of microorganisms on human health has long been acknowledged and studied, but recent advances in research methodologies have enabled a new systems-level perspective on the collections of microorganisms associated with humans, the human microbiome. Large-scale collaborative efforts such as the NIH Human Microbiome Project have sought to kick-start research on the human microbiome by providing foundational information on microbial composition based upon specific sites across the human body. Here, we focus on the four main anatomical sites of the human microbiome: gut, oral, skin, and vaginal, and provide information on site-specific background, experimental data, and computational modeling. Each of the site-specific microbiomes has unique organisms and phenomena associated with them; there are also high-level commonalities. By providing an overview of different human microbiome sites, we hope to provide a perspective where detailed, site-specific research is needed to understand causal phenomena that impact human health, but there is equally a need for more generalized methodology improvements that would benefit all human microbiome research.

Sign in / Sign up

Export Citation Format

Share Document