scholarly journals Enantioselective Hydrolysis of Racemic and Meso-Epoxides with Recombinant Escherichia coli Expressing Epoxide Hydrolase from Sphingomonas sp. HXN-200: Preparation of Epoxides and Vicinal Diols in High ee and High Concentration

ACS Catalysis ◽  
2013 ◽  
Vol 3 (4) ◽  
pp. 752-759 ◽  
Author(s):  
Shuke Wu ◽  
Aitao Li ◽  
Yit Siang Chin ◽  
Zhi Li
Keyword(s):  
Escherichia Coli ◽  
Epoxide Hydrolase ◽  
Recombinant Escherichia Coli ◽  
Enantioselective Hydrolysis ◽  
High Concentration ◽  
Vicinal Diols ◽  
Hydrolysis Of

Characterization of a Euphorbia lagascae epoxide hydrolase gene that is induced early during germination

2000 ◽  
Vol 28 (6) ◽  
pp. 855-856 ◽  
Author(s):  
J. Edqvist ◽  
I. Farbos
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Epoxide Hydrolase ◽  
Vernolic Acid ◽  
Major Fatty Acid ◽  
Transcription Pattern ◽  
Vicinal Diols ◽  
Hydrolysis Of

In Euphorbia lagascae the major fatty acid in triacylglycerol is the epoxidated fatty acid vernolic acid (cis- 12-epoxyoctadeca-cis-9-enoic acid). The enzymic reactions occurring during the catabolism of epoxidated fatty acids during germination are not known, but it seems likely that the degradation requires the activity of an epoxide hydrolase. Epoxide hydrolases are a group of functionally related enzymes that catalyse the cofactor-independent hydrolysis of epoxides to their corresponding vicinal diols by the addition of a water molecule. Here we report the cloning and characterization of an epoxide hydrolase gene from E. lagascae. The structure of the gene is unusual since it lacks introns. A detailed investigation of the transcription pattern of the epoxide hydrolase gene shows that the gene is induced during germination. We have used in situ hybridization to identify in which tissues the gene is expressed during germination. We speculate that this epoxide hydrolase enzyme is involved in the catabolism of epoxidated fatty acids during germination of E. lagascae seeds.

Enantioselective hydrolysis of racemic epichlorohydrin using an epoxide hydrolase from Novosphingobium aromaticivorans

2010 ◽  
Vol 110 (3) ◽  
pp. 295-297 ◽  
Author(s):  
Jung-Hee Woo ◽  
Young-Ok Hwang ◽  
Ji-Hyun Kang ◽  
Hyun Sook Lee ◽  
Sang-Jin Kim ◽  
...  
Keyword(s):  
Epoxide Hydrolase ◽  
Enantioselective Hydrolysis ◽  
Hydrolysis Of

Enantioselective hydrolysis of racemic styrene oxide by epoxide hydrolase ofRhodosporidium kratochvilovae SYU-08

2003 ◽  
Vol 8 (5) ◽  
pp. 306-308 ◽  
Author(s):  
Ji Won Lee ◽  
Eun Jung Lee ◽  
Seung Sik Yoo ◽  
Sung Hoon Park ◽  
Hee Sook Kim ◽  
...  
Keyword(s):  
Epoxide Hydrolase ◽  
Styrene Oxide ◽  
Enantioselective Hydrolysis ◽  
Hydrolysis Of
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