Activation Enhancement of Citric Acid Cycle to Promote Bioelectrocatalytic Activity of arcA Knockout Escherichia coli Toward High-Performance Microbial Fuel Cell

ACS Catalysis ◽  
2012 ◽  
Vol 2 (8) ◽  
pp. 1749-1752 ◽  
Author(s):  
Jing Liu ◽  
Yang-Chun Yong ◽  
Hao Song ◽  
Chang Ming Li
Keyword(s):  
Escherichia Coli ◽  
Fuel Cell ◽  
Citric Acid ◽  
Microbial Fuel Cell ◽  
High Performance ◽  
Citric Acid Cycle ◽  
Acid Cycle

scholarly journals The ms2io6A37 Modification of tRNA inSalmonella typhimurium Regulates Growth on Citric Acid Cycle Intermediates

1998 ◽  
Vol 180 (12) ◽  
pp. 3144-3151 ◽  
Author(s):  
Britt C. Persson ◽  
Ólafur Ólafsson ◽  
Hans K. Lundgren ◽  
Lars Hederstedt ◽  
Glenn R. Björk
Keyword(s):  
Escherichia Coli ◽  
Citric Acid ◽  
Salmonella Typhimurium ◽  
Gene Product ◽  
Respiratory Chain ◽  
Citric Acid Cycle ◽  
Dicarboxylic Acids ◽  
Acid Cycle ◽  
E Coli ◽  
Isopentenyl Adenosine

ABSTRACT The modified nucleoside 2-methylthio-N-6-isopentenyl adenosine (ms2i6A) is present in position 37 (adjacent to and 3′ of the anticodon) of tRNAs that read codons beginning with U except tRNA I,V Ser inEscherichia coli. In Salmonella typhimurium, 2-methylthio-N-6-(cis-hydroxy)isopentenyl adenosine (ms2io6A; also referred to as 2-methylthio cis-ribozeatin) is found in tRNA, most likely in the species that have ms2i6A in E. coli. Mutants (miaE) of S. typhimurium in which ms2i6A hydroxylation is blocked are unable to grow aerobically on the dicarboxylic acids of the citric acid cycle. Such mutants have normal uptake of dicarboxylic acids and functional enzymes of the citric acid cycle and the aerobic respiratory chain. The ability of S. typhimurium to grow on succinate, fumarate, and malate is dependent on the state of modification in position 37 of those tRNAs normally having ms2io6A37 and is not due to a second cellular function of tRNA (ms2io6A37)hydroxylase, themiaE gene product. We suggest that S. typhimurium senses the hydroxylation status of the isopentenyl group of the tRNA and will grow on succinate, fumarate, or malate only if the isopentenyl group is hydroxylated.

Sign in / Sign up

Export Citation Format

Share Document