Genetically Encoded Calcium Indicators

2008 ◽  
Vol 108 (5) ◽  
pp. 1550-1564 ◽  
Author(s):  
Marco Mank ◽  
Oliver Griesbeck
Keyword(s):  
Calcium Indicators ◽  
Genetically Encoded Calcium Indicators

scholarly journals CamelliA-based simultaneous imaging of Ca2+ dynamics in subcellular compartments

2021 ◽  
Author(s):  
Jingzhe Guo ◽  
Jiangman He ◽  
Katayoon Dehesh ◽  
Zhenbiao Yang
Keyword(s):  
High Resolution ◽  
Second Messenger ◽  
Wide Applicability ◽  
Simultaneous Imaging ◽  
Subcellular Compartments ◽  
Calcium Indicators ◽  
External Cues ◽  
Genetically Encoded Calcium Indicators ◽  
Arabidopsis Cells

As a universal second messenger, calcium (Ca2+) transmits specific cellular signals via a spatiotemporal signature generated from its extracellular source and internal stores. Our knowledge of the mechanisms underlying generation of a Ca2+ signature is hampered by limited tools enabling simultaneous monitoring of the dynamics of Ca2+ levels in multiple subcellular compartments. To overcome the limitation and to further improve spatiotemporal resolutions, here we have assembled a molecular toolset (the CamelliA lines) in Arabidopsis that enables simultaneous and high-resolution monitoring of Ca2+ dynamics in multiple subcellular compartments through imaging analyses of different single-colored GECIs (Genetically Encoded Calcium Indicators). Indeed, the uncovering of the previously unrecognized Ca2+ signatures in three types of Arabidopsis cells in response to internal and external cues is a testimony to the wide applicability of the newly generated toolset for elucidating the subcellular sources contributing to the Ca2+signatures in plants.

Evaluating the performance of genetically encoded calcium indicators expressed in mammalian neurons

2010 ◽  
Vol 68 ◽  
pp. e334
Author(s):  
Yoshiyuki Yamada ◽  
Takayuki Michikawa ◽  
Mitsuhiro Hashimoto ◽  
Atsushi Miyawaki ◽  
Katsuhiko Mikoshiba
Keyword(s):  
Calcium Indicators ◽  
Genetically Encoded Calcium Indicators

scholarly journals Imaging the response of the retina to electrical stimulation with genetically encoded calcium indicators

2013 ◽  
Vol 109 (7) ◽  
pp. 1979-1988 ◽  
Author(s):  
Andrew C. Weitz ◽  
Matthew R. Behrend ◽  
Nan Sook Lee ◽  
Ronald L. Klein ◽  
Vince A. Chiodo ◽  
...  
Keyword(s):  
Electrical Stimulation ◽  
Viral Vector ◽  
Ganglion Cells ◽  
High Specificity ◽  
Multielectrode Arrays ◽  
Limited Information ◽  
Stimulus Pulse ◽  
Photoreceptor Layer ◽  
Calcium Indicators ◽  
Genetically Encoded Calcium Indicators

Epiretinal implants for the blind are designed to stimulate surviving retinal neurons, thus bypassing the diseased photoreceptor layer. Single-unit or multielectrode recordings from isolated animal retina are commonly used to inform the design of these implants. However, such electrical recordings provide limited information about the spatial patterns of retinal activation. Calcium imaging overcomes this limitation, as imaging enables high spatial resolution mapping of retinal ganglion cell (RGC) activity as well as simultaneous recording from hundreds of RGCs. Prior experiments in amphibian retina have demonstrated proof of principle, yet experiments in mammalian retina have been hindered by the inability to load calcium indicators into mature mammalian RGCs. Here, we report a method for labeling the majority of ganglion cells in adult rat retina with genetically encoded calcium indicators, specifically GCaMP3 and GCaMP5G. Intravitreal injection of an adeno-associated viral vector targets ∼85% of ganglion cells with high specificity. Because of the large fluorescence signals provided by the GCaMP sensors, we can now for the first time visualize the response of the retina to electrical stimulation in real-time. Imaging transduced retinas mounted on multielectrode arrays reveals how stimulus pulse shape can dramatically affect the spatial extent of RGC activation, which has clear implications in prosthetic applications. Our method can be easily adapted to work with other fluorescent indicator proteins in both wild-type and transgenic mammals.

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