Right-First-Time Isocratic Preparative Liquid Chromatography-Mass Spectrometry Purification

2012 ◽  
Vol 14 (4) ◽  
pp. 273-279 ◽  
Author(s):  
Patrice Koza ◽  
Vanessa Gonnot ◽  
Jacques Pelleter
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Liquid Chromatography Mass Spectrometry ◽  
Preparative Liquid Chromatography ◽  
Chromatography Mass Spectrometry ◽  
First Time

scholarly journals Identification of Pyrrolizidine Alkaloids in Senecio Plants by Liquid Chromatography-Mass Spectrometry

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
An-Jing Lu ◽  
Yan-Liu Lu ◽  
Dao-Peng Tan ◽  
Lin Qin ◽  
Hua Ling ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Pyrrolizidine Alkaloids ◽  
Liquid Chromatography Mass Spectrometry ◽  
Research Information ◽  
Chemical Research ◽  
Chromatography Mass Spectrometry ◽  
Product Ions ◽  
First Time ◽  
Weight Number

Pyrrolizidine alkaloids (PAs) are considered as the major constituents that cause hepatoxicity in Senecio plants. PAs can be found in about 3%–5% of the world’s flowering plants. Nowadays, the identification method of PAs by separation and preparation was too slow and lacked effective power. A rapid method to identify PAs in plants must be developed. Based on the fragmentation regularity, the hepatoxic PAs and nonhepatoxic PAs were characterized by liquid chromatography-mass spectrometry (LC-MS). The detailed structures of PAs in five Senecio plants were identified based on tandem mass spectrometry (MS/MS) spectrum and chemical research information. In the present study, some new fragmentation regularities of PAs have been found, such as product ions at m/z 122, m/z 140 and m/z 124, m/z 142, which have been discovered as the characteristic fragments of lactone and mono-esterase type of saturated PAs, respectively. Moreover, two product ions at m/z 120 and m/z 138 have been reported as the characteristic fragments of unsaturated PAs. Some of them were found in Senecio species for the first time, and some of them may be new nature product or even new compound. Finally, we classified these plants into five categories based on PAs which were identified in the present study; the result corresponded with the classification by morphology. In addition, we have found some constituents that have odd molecular weight number only in Senecio species but not in Ligularia species; the detailed structures of these non-PAs constituents need penetrating study. LC-MS was rapid and sensitive method for detecting and identifying PAs in plants. Pyrrolizidine alkaloids were the toxiferous constituent of Senecio plants. In this study, we found that PAs can be used as the characteristic constituent of Senecio species.

Gas-Liquid Chromatography-Mass Spectrometry Investigation of Tropane Alkaloids in Hyoscyamus albus L. from Morocco

2012 ◽  
Vol 67 (9-10) ◽  
pp. 461-465 ◽  
Author(s):  
Ahmed El Bazaoui ◽  
My Ahmed Bellimam ◽  
Ibn Toumert Lançar ◽  
Abdelmajid Soulaymani
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tropane Alkaloids ◽  
Gas Liquid Chromatography ◽  
Liquid Chromatography Mass Spectrometry ◽  
Plant Organs ◽  
Chromatography Mass Spectrometry ◽  
New Compounds ◽  
First Time ◽  
Hyoscyamus Albus

Thirty-four alkaloids were identified in the organs of Hyoscyamus albus L. by gas-liquid chromatography-mass spectrometry (GLC-MS). Eight new compounds for the roots, eleven for the stems, twelve for the leaves, nineteen for the flowers, and seven for the seeds were detected. The alkaloids 5-(2-oxopropyl)-hygrine (8) and phygrine (20) are new for this species and 3-(hydroxyacetoxy)tropane (9), 6,7-dehydro-3-phenylacetoxytropane (15), 3-(2’-phenylpropionyloxy) tropane (17), 6,7-dehydro-3-apotropoyloxytropane (18), 3-(3’-methoxytropoyloxy)tropane (23), and aponorscopolamine (25) are described for the first time for the genus Hyoscyamus. Hyoscyamine was the main alkaloid in the plant organs

Analysis of herbicide atrazine and its degradation products in cereals by ultra-performance liquid chromatography-mass spectrometry

2016 ◽  
Vol 8 (17) ◽  
pp. 3599-3604 ◽  
Author(s):  
Shuxuan Liang ◽  
Xinfeng Dong ◽  
Ming Su ◽  
Hanwen Sun
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Degradation Products ◽  
Ultra Performance Liquid Chromatography ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Herbicide Atrazine ◽  
First Time

A novel ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) method was developed for the first time for the determination of herbicide atrazine (ATR) and its principal metabolites namely desisopropylatrazine (DIA), desethylatrazine (DEA) and hydroxyatrazine (HA) in cereals.

Identification of the impurities in 2,5-dimethoxy-4-ethylphenethylamine tablets by high performance liquid chromatography mass spectrometry-ion trap-time of flight

2016 ◽  
Vol 8 (46) ◽  
pp. 8179-8187 ◽  
Author(s):  
Yaning Li ◽  
Minchao Wang ◽  
Aoxin Li ◽  
Hui Zheng ◽  
Yun Wei
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Ion Trap ◽  
Time Of Flight ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
First Time

An efficient LC/MS-IT-TOF method to identify ten impurities and fragment pathways in 2C-E tablets was established for the first time.

Chiral liquid chromatography-mass spectrometry (LC-MS/MS) method development with β-cyclodextrin (β-CD) derivatized chiral stationary phase for the enhanced separation and determination of flurbiprofen enantiomers: application to a stereoselective pharmacokinetic study

2020 ◽  
Vol 44 (25) ◽  
pp. 10334-10342 ◽  
Author(s):  
Jiayi Sun ◽  
Beibei Liu ◽  
Liangzhao Cai ◽  
Jia Yu ◽  
Xingjie Guo
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Stationary Phase ◽  
Chiral Stationary Phase ◽  
Method Development ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
First Time ◽  
Flurbiprofen Enantiomers

Home-made chiral stationary phase for enhanced enantioselective separation and determination of flurbiprofen by liquid chromatography-mass spectrometry (LC-MS/MS) for the first time.

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