scholarly journals Uptake and Transport of Superparamagnetic Iron Oxide Nanoparticles through Human Brain Capillary Endothelial Cells

2013 ◽  
Vol 4 (10) ◽  
pp. 1352-1360 ◽  
Author(s):  
L. B. Thomsen ◽  
T. Linemann ◽  
K. M. Pondman ◽  
J. Lichota ◽  
K. S. Kim ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Iron Oxide ◽  
Human Brain ◽  
Iron Oxide Nanoparticles ◽  
Superparamagnetic Iron Oxide ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Brain Capillary ◽  
Capillary Endothelial Cells ◽  
Uptake And Transport

scholarly journals Activatable Superparamagnetic Iron Oxide Nanoparticles Scavenge Reactive Oxygen Species in Macrophages and Endothelial Cells

2020 ◽  
Author(s):  
Chukwuazam Nwasike ◽  
Eunsoo Yoo ◽  
Erin Purr ◽  
Amber L. Doiron
Keyword(s):  
Reactive Oxygen Species ◽  
Endothelial Cells ◽  
Iron Oxide ◽  
Contrast Agent ◽  
Iron Oxide Nanoparticles ◽  
Reactive Oxygen ◽  
Superparamagnetic Iron Oxide ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Oxygen Species

<p>This study centers around diagnostic medicine, and severity staging of inflammatory diseases. Previously, we showed that complexation of PEG and Poly(gallol) on superparamagnetic iron oxide nanoparticles turn OFF the MRI contrasting ability of the nanoparticle. However, in the presence of reactive oxygen species, the contrast agent will turn ON. In this article, for the first time, we provide evidence that our MRI contrast agent is sensitive to physiologically relevant ROS and induces antioxidant activity on immune and endothelial cells. This study provides initial evidence of IPC-SPIOs cellular ROS sensitivity and potential activatable properties in biological conditions.</p>

scholarly journals Activatable Superparamagnetic Iron Oxide Nanoparticles Scavenge Reactive Oxygen Species in Macrophages and Endothelial Cells

2020 ◽  
Author(s):  
Chukwuazam Nwasike ◽  
Eunsoo Yoo ◽  
Erin Purr ◽  
Amber L. Doiron
Keyword(s):  
Reactive Oxygen Species ◽  
Endothelial Cells ◽  
Iron Oxide ◽  
Contrast Agent ◽  
Iron Oxide Nanoparticles ◽  
Reactive Oxygen ◽  
Superparamagnetic Iron Oxide ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Oxygen Species

<p>This study centers around diagnostic medicine, and severity staging of inflammatory diseases. Previously, we showed that complexation of PEG and Poly(gallol) on superparamagnetic iron oxide nanoparticles turn OFF the MRI contrasting ability of the nanoparticle. However, in the presence of reactive oxygen species, the contrast agent will turn ON. In this article, for the first time, we provide evidence that our MRI contrast agent is sensitive to physiologically relevant ROS and induces antioxidant activity on immune and endothelial cells. This study provides initial evidence of IPC-SPIOs cellular ROS sensitivity and potential activatable properties in biological conditions.</p>

scholarly journals Activatable superparamagnetic iron oxide nanoparticles scavenge reactive oxygen species in macrophages and endothelial cells

RSC Advances ◽  
2020 ◽  
Vol 10 (68) ◽  
pp. 41305-41314
Author(s):  
Chukwuazam Nwasike ◽  
Eunsoo Yoo ◽  
Erin Purr ◽  
Amber L. Doiron
Keyword(s):  
Reactive Oxygen Species ◽  
Endothelial Cells ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Reactive Oxygen ◽  
Superparamagnetic Iron Oxide ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Oxygen Species ◽  
Intracellular Ros

Complexed IPC-SPIOs scavenge intracellular ROS after internalization.

Biocompatible, Superparamagnetic, Flame Synthesized Iron Oxide Nanoparticles: Cellular Uptake and Toxicity Studies

2008 ◽  
Author(s):  
Kivilcim Buyukhatipoglu ◽  
Tiffany A. Miller ◽  
Alisa Morss Clyne
Keyword(s):  
Particle Size ◽  
Endothelial Cells ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Superparamagnetic Iron Oxide ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Synthesis Process ◽  
Flame Synthesis ◽  
Oxide Nanoparticles ◽  
Biological Applications

Superparamagnetic iron oxide nanoparticles, including magnetite (Fe3O4), are widely used in applications such as targeted drug delivery, magnetic resonance imaging, tissue engineering, gene therapy, hyperthermic malignant cell treatment, and cell membrane manipulation. These nanoparticles are particularly interesting for in vivo and in vitro applications since they do not exhibit magnetic behavior once the magnetic field has been removed. In the current work, superparamagnetic iron oxide nanoparticles were produced using a flame synthesis method, which provides significant advantages over other material synthesis processes such as solgel processing, chemical vapor deposition, and laser ablation. Flame synthesis allows control of particle size, size distribution, phase and composition by altering flame operating conditions. Flame synthesis is further capable of commercial production rates with minimal post-processing of the final product materials. This study focuses on the interaction of flame synthesized iron oxide nanoparticles with porcine aortic endothelial cells and compares the results to those obtained using commercially available iron oxide nanoparticles. The materials characteristics of the flame synthesized iron oxide nanoparticles, including morphology, elemental composition, particle size, were analyzed by electron microscopy (TEM, ESEM, EDS), and Raman Spectroscopy. The data verified production of a heterogenous mixture of hematite and magnetite nanoparticles, which exhibit superparamagnetic properties. Monodisperse iron oxide particles of 6–12 nm diameter and aggregated clusters of these 6–12nm nanoparticles have been synthesized. Nanoparticle biocompatibility was assessed by incubating flame synthesized and commercially available iron oxide nanoparticles with endothelial cells for 24 hours. Both alamar blue and Live/Dead cell assays showed no significant toxicity difference between flame synthesized and commercially available nanoparticles. Cells exposed to both types of nanoparticles maintained membrane integrity, as indicated by minimal lactase dehydrogenase release. Endothelial cells imaged by ESEM and confirmed by EDS demonstrated that uncoated flame synthesized nanoparticles are ingested into cells in a similar manner to commercially available nanoparticles. These data suggest that flame synthesized iron oxide nanoparticles are comparable to commercially available nanoparticles for biological applications. Flame synthesis has the advantage of a relatively simple synthesis process with higher purity products and lower time and energy manufacturing costs. Future work will include functionalizing the nanoparticle surfaces for specific biological applications, including specific cell targeting and bioactive factor delivery.

Comparison between Dextran-Coated Superparamagnetic Iron Oxide Nanoparticles and Magnetoliposome on Rabbit Corneal Endothelial Cells Labeling

2013 ◽  
Vol 652-654 ◽  
pp. 234-240
Author(s):  
Ming Feng Wu ◽  
Li Xia Lu ◽  
Xiao Ting Sun ◽  
Fei Du ◽  
Yan Long Bi
Keyword(s):  
Magnetic Field ◽  
Endothelial Cells ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Superparamagnetic Iron Oxide ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Blue Staining ◽  
Iron Oxide Nanoparticle ◽  
Oxide Nanoparticles ◽  
Corneal Endothelial Cells

Purpose: To evaluate and compare the promising applications of dextran-coated superparamagnetic iron oxide nanoparticle (dSPION) and magnetoliposome on corneal endothelial cells transplant therapy. Methods: Two kinds of novel superparamagnetic iron oxide nanoparticles (SPIONs), dSPION and magnetoliposome were synthesized, characterized and incorporated to rabbit corneal endothelial cells (RCECs). Uptakes of SPIONs were identified by Prussian blue staining. Cells migrating rates in magnetic field were calculated. Cytotoxicities of the two SPIONs were detected using MTT assay. Result: Magrating rates of dSPION (4 ~ 64 μg/mL) labled RCECs were 24.1±2.4% ~ 78.3±2.2%, and 5.0±1.7% of unlabeled RCECs. (p0.05) Conclusion: RCECs labeled with each SPION migrated significantly in external magnetic field, while cytotoxicities did not revealed. Our study demonstrated the potential applications of dSPION and magnetoliposome on labeling RCECs for cell transplantation.

TARGETING CYCLIN B1 WITH ANTISENSE OLIGONUCLETOTIDES- COATED SUPERPARAMAGNETIC IRON OXIDE NANOPARTICLES

2018 ◽  
Vol 6 (10) ◽  
Author(s):  
Hosam Zaghloul ◽  
Doaa A. Shahin ◽  
Ibrahim El- Dosoky ◽  
Mahmoud E. El-awady ◽  
Fardous F. El-Senduny ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Cellular Uptake ◽  
Superparamagnetic Iron Oxide ◽  
Cyclin B1 ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Mcf7 Cells ◽  
M Phase ◽  
The Impact

Antisense oligonucleotides (ASO) represent an attractive trend as specific targeting molecules but sustain poor cellular uptake meanwhile superparamagnetic iron oxide nanoparticles (SPIONs) offer stability of ASO and improved cellular uptake. In the present work we aimed to functionalize SPIONs with ASO targeting the mRNA of Cyclin B1 which represents a potential cancer target and to explore its anticancer activity. For that purpose, four different SPIONs-ASO conjugates, S-M (1–4), were designated depending on the sequence of ASO and constructed by crosslinking carboxylated SPIONs to amino labeled ASO. The impact of S-M (1–4) on the level of Cyclin B1, cell cycle, ROS and viability of the cells were assessed by flowcytometry. The results showed that S-M3 and S-M4 reduced the level of Cyclin B1 by 35 and 36%, respectively. As a consequence to downregulation of Cyclin B1, MCF7 cells were shown to be arrested at G2/M phase (60.7%). S-M (1–4) led to the induction of ROS formation in comparison to the untreated control cells. Furthermore, S-M (1–4) resulted in an increase in dead cells compared to the untreated cells and SPIONs-treated cells. In conclusion, targeting Cyclin B1 with ASO-coated SPIONs may represent a specific biocompatible anticancer strategy.

Sign in / Sign up

Export Citation Format

Share Document