scholarly journals Adsorption of α-Synuclein to Supported Lipid Bilayers: Positioning and Role of Electrostatics

2013 ◽  
Vol 4 (10) ◽  
pp. 1339-1351 ◽  
Author(s):  
Erik Hellstrand ◽  
Marie Grey ◽  
Marie-Louise Ainalem ◽  
John Ankner ◽  
V. Trevor Forsyth ◽  
...  
Keyword(s):  
Lipid Bilayers ◽  
Supported Lipid Bilayers

scholarly journals The role of surface charge in the interaction of nanoparticles with model pulmonary surfactants

Soft Matter ◽  
2018 ◽  
Vol 14 (28) ◽  
pp. 5764-5774 ◽  
Author(s):  
F. Mousseau ◽  
J.-F. Berret
Keyword(s):  
Surface Charge ◽  
Lipid Bilayers ◽  
Pulmonary Surfactant ◽  
Lipid Vesicles ◽  
Electrostatic Charge ◽  
Supported Lipid Bilayers ◽  
Pulmonary Surfactants ◽  
Inhaled Nanoparticles

Inhaled nanoparticles reaching the respiratory zone in the lungs enter first in contact with the pulmonary surfactant. It is shown here that nanoparticles and lipid vesicles formulated from different surfactant mimetics interact predominantlyviaelectrostatic charge mediated attraction and do not form supported lipid bilayers spontaneously.

Microenvironment alterations enhance photocurrents from photosystem I confined in supported lipid bilayers

2018 ◽  
Vol 6 (26) ◽  
pp. 12281-12290 ◽  
Author(s):  
Hanieh Niroomand ◽  
Ravi Pamu ◽  
Dibyendu Mukherjee ◽  
Bamin Khomami
Keyword(s):  
Lipid Bilayers ◽  
Photosystem I ◽  
Charge Separation ◽  
Critical Role ◽  
Supported Lipid Bilayers ◽  
Induced Charge

This work elucidates the role of natural membrane confinements of photosystem I complexes (PSI) in light-induced charge separation by comparing the photocurrents of isolated PSI with PSI encapsulated within solid-supported lipid bilayers on electrodes. Our results indicate the critical role of the microenvironment alterations in achieving enhanced photocurrent and stability.

scholarly journals Perturbation of Hydrogen Bonding Networks over Supported Lipid Bilayers by Poly (Allylamine Hydrochloride)

2019 ◽  
Author(s):  
Naomi Dalchand ◽  
Merve Dogangun ◽  
Paul E. Ohno ◽  
Emily Ma ◽  
Alex Martinson ◽  
...  
Keyword(s):  
Lipid Bilayers ◽  
Electrostatic Interactions ◽  
Supported Lipid Bilayers ◽  
Water Molecules ◽  
Interfacial Water ◽  
Sum Frequency ◽  
Biochemical Processes ◽  
Zwitterionic Lipids ◽  
Allylamine Hydrochloride

<div><div><div><p>Water is vital to many biochemical processes and is necessary for driving many fundamental interactions of cell membranes with their external environments, yet it is difficult to probe the membrane/water interface directly and without the use of external labels. Here, we employ vibrational sum frequency generation (SFG) spectroscopy to understand the role of interfacial water molecules above bilayers formed from zwitterionic (phosphatidylcholine, PC) and anionic (phosphatidylglycerol, PG, and phosphatidylserine, PS) lipids as they are exposed to the common polycation poly (allylamine hydrochloride) (PAH) in 100 mM NaCl. We show that as the concentration of PAH is increased, the interfacial water molecules are irreversibly displaced and find that it requires 10 times more PAH to displace interfacial water molecules from membranes formed from purely zwitterionic lipids when compared to membranes that contain the anionic PG and PS lipids. This outcome is likely due to difference in (1) the energy with which water molecules are bound to the lipid headgroups, (2) the number of water molecules bound to the headgroups, which is related to the headgroup area, and (3) the electrostatic interactions between the PAH molecules and the negatively charged lipids that are favored when compared to the zwitterionic lipid headgroups. The findings presented here contribute to establishing causal relationships in nanotoxicology and to understanding, controlling, and predicting the initial steps that lead to the lysis of cells exposed to membrane disrupting polycations, or to transfection.</p></div></div></div>

scholarly journals Clustering of α-Synuclein on Supported Lipid Bilayers: Role of Anionic Lipid, Protein, and Divalent Ion Concentration

2009 ◽  
Vol 96 (2) ◽  
pp. 540-551 ◽  
Author(s):  
Anjan P. Pandey ◽  
Farzin Haque ◽  
Jean-Christophe Rochet ◽  
Jennifer S. Hovis
Keyword(s):  
Lipid Bilayers ◽  
Ion Concentration ◽  
Supported Lipid Bilayers ◽  
Anionic Lipid

scholarly journals Perturbation of Hydrogen Bonding Networks over Supported Lipid Bilayers by Poly (Allylamine Hydrochloride)

2019 ◽  
Author(s):  
Naomi Dalchand ◽  
Merve Dogangun ◽  
Paul E. Ohno ◽  
Emily Ma ◽  
Alex Martinson ◽  
...  
Keyword(s):  
Lipid Bilayers ◽  
Electrostatic Interactions ◽  
Supported Lipid Bilayers ◽  
Water Molecules ◽  
Interfacial Water ◽  
Sum Frequency ◽  
Biochemical Processes ◽  
Zwitterionic Lipids ◽  
Allylamine Hydrochloride

<div><div><div><p>Water is vital to many biochemical processes and is necessary for driving many fundamental interactions of cell membranes with their external environments, yet it is difficult to probe the membrane/water interface directly and without the use of external labels. Here, we employ vibrational sum frequency generation (SFG) spectroscopy to understand the role of interfacial water molecules above bilayers formed from zwitterionic (phosphatidylcholine, PC) and anionic (phosphatidylglycerol, PG, and phosphatidylserine, PS) lipids as they are exposed to the common polycation poly (allylamine hydrochloride) (PAH) in 100 mM NaCl. We show that as the concentration of PAH is increased, the interfacial water molecules are irreversibly displaced and find that it requires 10 times more PAH to displace interfacial water molecules from membranes formed from purely zwitterionic lipids when compared to membranes that contain the anionic PG and PS lipids. This outcome is likely due to difference in (1) the energy with which water molecules are bound to the lipid headgroups, (2) the number of water molecules bound to the headgroups, which is related to the headgroup area, and (3) the electrostatic interactions between the PAH molecules and the negatively charged lipids that are favored when compared to the zwitterionic lipid headgroups. The findings presented here contribute to establishing causal relationships in nanotoxicology and to understanding, controlling, and predicting the initial steps that lead to the lysis of cells exposed to membrane disrupting polycations, or to transfection.</p></div></div></div>

Influence of Brain Gangliosides on the Formation and Properties of Supported Lipid Bilayers for Binding Kinetics Studies

2018 ◽  
Author(s):  
Luke Jordan ◽  
Nathan Wittenberg
Keyword(s):  
Lipid Bilayers ◽  
Binding Kinetics ◽  
Supported Lipid Bilayers ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Supported Lipid Bilayer ◽  
Head Groups ◽  
Lipid Diffusion ◽  
Kinetics Of ◽  
Brain Gangliosides

This is a comprehensive study of the effects of the four major brain gangliosides (GM1, GD1b, GD1a, and GT1b) on the adsorption and rupture of phospholipid vesicles on SiO2 surfaces for the formation of supported lipid bilayer (SLB) membranes. Using quartz crystal microbalance with dissipation monitoring (QCM-D) we show that gangliosides GD1a and GT1b significantly slow the SLB formation process, whereas GM1 and GD1b have smaller effects. This is likely due to the net ganglioside charge as well as the positions of acidic sugar groups on ganglioside glycan head groups. Data is included that shows calcium can accelerate the formation of ganglioside-rich SLBs. Using fluorescence recovery after photobleaching (FRAP) we also show that the presence of gangliosides significantly reduces lipid diffusion coefficients in SLBs in a concentration-dependent manner. Finally, using QCM-D and GD1a-rich SLB membranes we measure the binding kinetics of an anti-GD1a antibody that has similarities to a monoclonal antibody that is a hallmark of a variant of Guillain-Barre syndrome.

scholarly journals Silica nanoparticle supported lipid bilayers for gene delivery

2009 ◽  
pp. 5100 ◽  
Author(s):  
Juewen Liu ◽  
Alison Stace-Naughton ◽  
C. Jeffrey Brinker
Keyword(s):  
Gene Delivery ◽  
Lipid Bilayers ◽  
Silica Nanoparticle ◽  
Supported Lipid Bilayers

scholarly journals Lipid Transporters Beam Signals from Cell Membranes

Membranes ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 562
Author(s):  
Miliça Ristovski ◽  
Danny Farhat ◽  
Shelly Ellaine M. Bancud ◽  
Jyh-Yeuan Lee
Keyword(s):  
Membrane Proteins ◽  
Lipid Bilayers ◽  
Lipid Composition ◽  
Structural Integrity ◽  
Current Knowledge ◽  
Integral Membrane Proteins ◽  
Cellular Membranes ◽  
Cytoplasmic Proteins ◽  
Lipid Transporters

Lipid composition in cellular membranes plays an important role in maintaining the structural integrity of cells and in regulating cellular signaling that controls functions of both membrane-anchored and cytoplasmic proteins. ATP-dependent ABC and P4-ATPase lipid transporters, two integral membrane proteins, are known to contribute to lipid translocation across the lipid bilayers on the cellular membranes. In this review, we will highlight current knowledge about the role of cholesterol and phospholipids of cellular membranes in regulating cell signaling and how lipid transporters participate this process.

scholarly journals Formation of Supported Lipid Bilayers Derived from Vesicles of Various Compositional Complexity on Conducting Polymer/Silica Substrates

Langmuir ◽  
2021 ◽  
Author(s):  
Hanna Ulmefors ◽  
Josefin Nissa ◽  
Hudson Pace ◽  
Olov Wahlsten ◽  
Anders Gunnarsson ◽  
...  
Keyword(s):  
Lipid Bilayers ◽  
Conducting Polymer ◽  
Supported Lipid Bilayers ◽  
Silica Substrates
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