scholarly journals In Vivo Calibration of Microdialysis Using Infusion of Stable-Isotope Labeled Neurotransmitters

2013 ◽  
Vol 4 (5) ◽  
pp. 729-736 ◽  
Author(s):  
Neil D. Hershey ◽  
Robert T. Kennedy
Keyword(s):  
Stable Isotope ◽  
In Vivo Calibration

scholarly journals Physiological impact of in vivo stable isotope tracing on cancer metabolism

2021 ◽  
pp. 101294
Author(s):  
Manuel Grima-Reyes ◽  
Adriana Martinez-Turtos ◽  
Ifat Abramovich ◽  
Eyal Gottlieb ◽  
Johanna Chiche ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Cancer Metabolism ◽  
Isotope Tracing ◽  
Physiological Impact

scholarly journals In vivo calibration of the T2* cardiovascular magnetic resonance method at 1.5 T for estimation of cardiac iron in a minipig model of transfusional iron overload

2021 ◽  
Vol 23 (1) ◽  
Author(s):  
Peter Diedrich Jensen ◽  
Asbjørn Haaning Nielsen ◽  
Carsten Wiberg Simonsen ◽  
Ulrik Thorngren Baandrup ◽  
Svend Eggert Jensen ◽  
...  
Keyword(s):  
Rank Correlation ◽  
Calibration Data ◽  
Iron Loading ◽  
Spearman Rank Correlation ◽  
Calibration Equation ◽  
Spearman Rank ◽  
Cardiac Iron ◽  
In Vivo Calibration ◽  
Spearman Rank Correlation Coefficient

Abstract Background Non-invasive estimation of the cardiac iron concentration (CIC) by T2* cardiovascular magnetic resonance (CMR) has been validated repeatedly and is in widespread clinical use. However, calibration data are limited, and mostly from post-mortem studies. In the present study, we performed an in vivo calibration in a dextran-iron loaded minipig model. Methods R2* (= 1/T2*) was assessed in vivo by 1.5 T CMR in the cardiac septum. Chemical CIC was assessed by inductively coupled plasma-optical emission spectroscopy in endomyocardial catheter biopsies (EMBs) from cardiac septum taken during follow up of 11 minipigs on dextran-iron loading, and also in full-wall biopsies from cardiac septum, taken post-mortem in another 16  minipigs, after completed iron loading. Results A strong correlation could be demonstrated between chemical CIC in 55 EMBs and parallel cardiac T2* (Spearman rank correlation coefficient 0.72, P < 0.001). Regression analysis led to [CIC] = (R2* − 17.16)/41.12 for the calibration equation with CIC in mg/g dry weight and R2* in Hz. An even stronger correlation was found, when chemical CIC was measured by full-wall biopsies from cardiac septum, taken immediately after euthanasia, in connection with the last CMR session after finished iron loading (Spearman rank correlation coefficient 0.95 (P < 0.001). Regression analysis led to the calibration equation [CIC] = (R2* − 17.2)/31.8. Conclusions Calibration of cardiac T2* by EMBs is possible in the minipig model but is less accurate than by full-wall biopsies. Likely explanations are sampling error, variable content of non-iron containing tissue and smaller biopsies, when using catheter biopsies. The results further validate the CMR T2* technique for estimation of cardiac iron in conditions with iron overload and add to the limited calibration data published earlier.

scholarly journals Comprehensive assessment of post-prandial protein handling by the application of intrinsically labelled protein in vivo in human subjects

2021 ◽  
pp. 1-9
Author(s):  
Jorn Trommelen ◽  
Andrew M. Holwerda ◽  
Philippe J. M. Pinckaers ◽  
Luc J. C. van Loon
Keyword(s):  
Protein Synthesis ◽  
Amino Acid ◽  
Stable Isotope ◽  
Dietary Protein ◽  
Protein Metabolism ◽  
Muscle Protein ◽  
Human Subjects ◽  
Whole Body ◽  
Body Protein

All human tissues are in a constant state of remodelling, regulated by the balance between tissue protein synthesis and breakdown rates. It has been well-established that protein ingestion stimulates skeletal muscle and whole-body protein synthesis. Stable isotope-labelled amino acid methodologies are commonly applied to assess the various aspects of protein metabolism in vivo in human subjects. However, to achieve a more comprehensive assessment of post-prandial protein handling in vivo in human subjects, intravenous stable isotope-labelled amino acid infusions can be combined with the ingestion of intrinsically labelled protein and the collection of blood and muscle tissue samples. The combined application of ingesting intrinsically labelled protein with continuous intravenous stable isotope-labelled amino acid infusion allows the simultaneous assessment of protein digestion and amino acid absorption kinetics (e.g. release of dietary protein-derived amino acids into the circulation), whole-body protein metabolism (whole-body protein synthesis, breakdown and oxidation rates and net protein balance) and skeletal muscle metabolism (muscle protein fractional synthesis rates and dietary protein-derived amino acid incorporation into muscle protein). The purpose of this review is to provide an overview of the various aspects of post-prandial protein handling and metabolism with a focus on insights obtained from studies that have applied intrinsically labelled protein under a variety of conditions in different populations.

scholarly journals Stable isotope labelling in vivo as an aid to protein identification in peptide mass fingerprinting

PROTEOMICS ◽  
2002 ◽  
Vol 2 (2) ◽  
pp. 157-163 ◽  
Author(s):  
Julie M. Pratt ◽  
Duncan H. L. Robertson ◽  
Simon J. Gaskell ◽  
Isabel Riba-Garcia ◽  
Simon J. Hubbard ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Protein Identification ◽  
Peptide Mass Fingerprinting ◽  
Stable Isotope Labelling ◽  
Isotope Labelling ◽  
Peptide Mass

Stable isotope tracing to assess tumor metabolism in vivo

2021 ◽  
Author(s):  
Brandon Faubert ◽  
Alpaslan Tasdogan ◽  
Sean J. Morrison ◽  
Thomas P. Mathews ◽  
Ralph J. DeBerardinis
Keyword(s):  
Stable Isotope ◽  
Tumor Metabolism ◽  
Isotope Tracing
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