Probabilistic Kinetic Model of Slow Oxidation of Low-Density Lipoprotein. 3. Hydroperoxide-Free Initiation

2005 ◽  
Vol 45 (6) ◽  
pp. 1616-1620 ◽  
Author(s):  
Dubravka Krilov ◽  
Janko N. Herak
Keyword(s):  
Kinetic Model ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density

Low density lipoprotein turnover in swine

1978 ◽  
Vol 56 (10) ◽  
pp. 963-967 ◽  
Author(s):  
Y. L. Marcel ◽  
A. C. Nestruck ◽  
M. Bergseth ◽  
M. Bidallier ◽  
W. T. Robinson ◽  
...  
Keyword(s):  
Kinetic Model ◽  
Decay Curve ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Exponential Model ◽  
Low Density ◽  
Miniature Swine ◽  
Synthetic Rate ◽  
Catabolic Rate ◽  
The Mean

The catabolism of intravenously injected 125I-labelled low density lipoproteins (LDL) was followed in normal miniature swine for 2 weeks. When compared with the two-exponential model, the decay curve of the plasma radioactivity associated with the LDL fraction was best described by a three-exponential model. In this system, the half-lives were 4.5 ± 3.7, 19.7 ± 6.6, and 127 ± 70 h (mean of four studies).Assuming a kinetic model with metabolism of LDL in the rapidly equilibrating compartment and two slower equilibrating compartments (a model requiring three exponentials), the mean fractional catabolic rate for apo-LDL was calculated to be 0.015 h−1. Therefore, if at steady state, the synthetic rate for apo-LDL in the same pigs would be 5.6 ± 4.1 mg/h. Different kinetic models using two or three exponentials would provide different value s for the synthetic rate of apo-LDL.However, in view of the known existence of at least three major equilibrating pools for LDL in plasma, liver, and lymph, and in view of the present results, the kinetic model for LDL metabolism should be better represented by a three-exponential system.

Probabilistic kinetic model of slow oxidation of low-density lipoprotein: I. Theory

2004 ◽  
Vol 129 (1) ◽  
pp. 63-74 ◽  
Author(s):  
Janko N Herak ◽  
Nataša Stojanović ◽  
Dubravka Krilov
Keyword(s):  
Kinetic Model ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density

Intralysosomal Accumulation of Colloidal Gold-Low Density Lipoprotein Conjugates in Chloroquine-Treated Fibroblasts

1985 ◽  
Vol 43 ◽  
pp. 546-547 ◽  
Author(s):  
Dean A. Handley ◽  
Cynthia M. Arbeeny ◽  
Larry D. Witte
Keyword(s):  
Colloidal Gold ◽  
Cultured Cells ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Coated Vesicle ◽  
Low Density ◽  
Cholesterol Esters ◽  
Cultured Fibroblasts ◽  
Surface Receptors ◽  
Ldl Particles

Low density lipoproteins (LDL) are the major cholesterol carrying particles in the blood. Using cultured cells, it has been shown that LDL particles interact with specific surface receptors and are internalized via a coated pit-coated vesicle pathway for lysosomal catabolism. This (Pathway has been visualized using LDL labeled to ferritin or colloidal gold. It is now recognized that certain lysomotropic agents, such as chloroquine, inhibit lysosomal enzymes that degrade protein and cholesterol esters. By interrupting cholesterol ester hydrolysis, chloroquine treatment results in lysosomal accumulation of cholesterol esters from internalized LDL. Using LDL conjugated to colloidal gold, we have examined the ultrastructural effects of chloroquine on lipoprotein uptake by normal cultured fibroblasts.

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