High-Throughput Modeling of Human G-Protein Coupled Receptors:  Amino Acid Sequence Alignment, Three-Dimensional Model Building, and Receptor Library Screening

2004 ◽  
Vol 44 (3) ◽  
pp. 1162-1176 ◽  
Author(s):  
Caterina Bissantz ◽  
Antoine Logean ◽  
Didier Rognan
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Model Building ◽  
Three Dimensional ◽  
G Protein Coupled Receptors ◽  
Library Screening ◽  
Dimensional Model ◽  
Three Dimensional Model ◽  
Amino Acid Sequence Alignment ◽  
G Protein Coupled

Computer Aided Protein Design: Three Dimensional Model Building of the Saruplase Structure

1991 ◽  
pp. 159-166
Author(s):  
W. Straßburger ◽  
W. Winter ◽  
G. J. Steffens ◽  
W. A. Günzler ◽  
L. Flohé
Keyword(s):  
Protein Design ◽  
Model Building ◽  
Three Dimensional ◽  
Dimensional Model ◽  
Three Dimensional Model ◽  
Computer Aided

scholarly journals Amino-acid sequence and predicted three-dimensional structure of pea seed (Pisum sativum) ferritin

1992 ◽  
Vol 288 (3) ◽  
pp. 931-939 ◽  
Author(s):  
S Lobreaux ◽  
S J Yewdall ◽  
J F Briat ◽  
P M Harrison
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Three Dimensional ◽  
Transit Peptide ◽  
Amino Acid Identity ◽  
Dimensional Structure ◽  
Three Dimensional Model ◽  
Iron Storage ◽  
Liver Ferritin ◽  
Pea Seed

The iron storage protein, ferritin, is widely distributed in the living kingdom. Here the complete cDNA and derived amino-acid sequence of pea seed ferritin are described, together with its predicted secondary structure, namely a four-helix-bundle fold similar to those of mammalian ferritins, with a fifth short helix at the C-terminus. An N-terminal extension of 71 residues contains a transit peptide (first 47 residues) responsible for plastid targetting as in other plant ferritins, and this is cleaved before assembly. The second part of the extension (24 residues) belongs to the mature subunit; it is cleaved during germination. The amino-acid sequence of pea seed ferritin is aligned with those of other ferritins (49% amino-acid identity with H-chains and 40% with L-chains of human liver ferritin in the aligned region). A three-dimensional model has been constructed by fitting the aligned sequence to the coordinates of human H-chains, with appropriate modifications. A folded conformation with an 11-residue helix is predicted for the N-terminal extension. As in mammalian ferritins, 24 subunits assemble into a hollow shell. In pea seed ferritin, its N-terminal extension is exposed on the outside surface of the shell. Within each pea subunit is a ferroxidase centre resembling those of human ferritin H-chains except for a replacement of Glu-62 by His. The channel at the 4-fold-symmetry axes defined by E-helices, is predicted to be hydrophilic in plant ferritins, whereas it is hydrophobic in mammalian ferritins.

Architecture and Mathematics

1996 ◽  
Vol 89 (6) ◽  
pp. 456-458
Author(s):  
Dan Reif
Keyword(s):  
Model Building ◽  
Three Dimensional ◽  
Dimensional Model ◽  
Three Dimensional Model ◽  
Architecture And Mathematics ◽  
Mathematics Skills ◽  
Scale Models ◽  
And Mathematics

It's got to have a center staircase!” “I've always wanted a kitchen with an island!” “Where is the best place for a skylight?” Enthusiastic comments such as these were typical of those I overheard while conducting a two-week workshop in which students used three-dimensional-model-building kits to plan and construct detailed scale models of their own home designs and to develop and sharpen mathematics skills related to architecture.

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