Amino Acidic Control of Calcium Phosphate Precipitation by Using the Vapor Diffusion Method in Microdroplets

2011 ◽  
Vol 11 (11) ◽  
pp. 4802-4809 ◽  
Author(s):  
Jaime Gómez-Morales ◽  
José Manuel Delgado-López ◽  
Michele Iafisco ◽  
Angeles Hernández-Hernández ◽  
María Prat
Keyword(s):  
Calcium Phosphate ◽  
Diffusion Method ◽  
Vapor Diffusion ◽  
Calcium Phosphate Precipitation

Evolution of calcium phosphate precipitation in hanging drop vapor diffusion by in situ Raman microspectroscopy

CrystEngComm ◽  
2013 ◽  
Vol 15 (12) ◽  
pp. 2206 ◽  
Author(s):  
Gloria Belén Ramírez-Rodríguez ◽  
José Manuel Delgado-López ◽  
Jaime Gómez-Morales
Keyword(s):  
Calcium Phosphate ◽  
Raman Microspectroscopy ◽  
Hanging Drop ◽  
Vapor Diffusion ◽  
In Situ Raman ◽  
Calcium Phosphate Precipitation

scholarly journals Transfection of Capsaspora owczarzaki  using calcium-phosphate precipitation v1 (protocols.io.p4adqse)

protocols.io ◽  
2018 ◽  
Author(s):  
Helena Parra ◽  
N ria ◽  
Alberto Perez ◽  
Aleksandra Kozyczkowska ◽  
Sebasti n
Keyword(s):  
Calcium Phosphate ◽  
Calcium Phosphate Precipitation

A gradual desiccation method for improving the efficiency of protein crystallization screening

2012 ◽  
Vol 45 (4) ◽  
pp. 758-765 ◽  
Author(s):  
Qin-Qin Lu ◽  
Xu-Zhuo Xie ◽  
Rui-Qing Chen ◽  
Zi-Qing Wu ◽  
Qing-Di Cheng ◽  
...  
Keyword(s):  
Success Rate ◽  
Protein Crystallization ◽  
Diffusion Method ◽  
Powerful Method ◽  
Vapor Diffusion ◽  
Optimal Amount ◽  
Reservoir Solution ◽  
The One

In vapor diffusion protein crystallization screening, it has been reported that replacing the reservoir solution with desiccant can increase the crystallization success rate. Therefore, the desiccation method is a potentially powerful method in practical protein crystallization screening. However, this method is difficult to apply broadly because the optimal amount of desiccant for a specific screening task is unknown. Utilizing an unsuitable amount of desiccant can result in even worse screening results than would be obtained from the traditional vapor diffusion method. Here, it is shown that by employing a modified strategy, named the gradual desiccation method, the problem can be solved without knowing the optimal amount of desiccant, and the crystallization success rate can be further increased compared with the one-time desiccation method.

Molecular Basis of Salivary Proline-rich Protein and Peptide Synthesis: Cell-free Translations and Processing of Human and Macaque Statherin mRNAs and Partial Amino Acid Sequence of their Signal Peptides

1987 ◽  
Vol 66 (2) ◽  
pp. 462-466 ◽  
Author(s):  
F.G. Oppenheim ◽  
D.I. Hay ◽  
D.J. Smith ◽  
G.D. Offner ◽  
R.F. Troxler
Keyword(s):  
Calcium Phosphate ◽  
Macaca Fascicularis ◽  
Electrophoretic Analysis ◽  
Proteolytic Processing ◽  
Oral Environment ◽  
Reticulocyte Lysate ◽  
Polyprotein Precursor ◽  
Phosphate Salts ◽  
Calcium Phosphate Precipitation

Acidic proline-rich phosphoproteins and phosphopeptides are abundant components of parotid and submandibular salivary secretions in man and in the subhuman primate, Macaca fascicularis. The major acidic proline-rich proteins and the proline-rich phosphopeptide, statherin, of man and macaques have been shown to be potent inhibitors of calcium phosphate precipitation and are thought to function in the oral environment by maintaining saliva supersaturated with respect to calcium phosphate salts. Little is known about the biosynthesis of these proline-rich phosphoproteins and peptides, and the aim of the present work was to determine the structural relationship between statherin precursors and native human and macaque statherin. RNA was isolated from human submandibular gland, and poly(A+) mRNA was selected by affinity chromatography on oligo(dT) cellulose and translated in a reticulocyte lysate. Electrophoretic analysis of the translation products revealed that this mRNA directed the synthesis of a large number of polypeptides with M,s ranging from 5000 to 70,000. Immunoprecipitates, prepared with an antiserum directed against human statherin, contained a single component with a Mr of 7800, approximately 2000 daltons larger than native statherin. Radiosequencing of the in vitro precursor of statherin in immunoprecipitates demonstrated the presence of a 19-residue signal peptide. These results suggest that statherin is derived from a unique structural gene, and does not result from proteolytic processing of a large polyprotein precursor.

Effect of Electrical Polarization on the Behavior of Bioactive Glass Containing MgO and B2O3 in SBF

2006 ◽  
Vol 309-311 ◽  
pp. 333-336
Author(s):  
Emiko Amaoka ◽  
Erik Vedel ◽  
Satoshi Nakamura ◽  
Yusuke Moriyoshi ◽  
Jukka I. Salonen ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Simulated Body Fluid ◽  
Bioactive Glass ◽  
Body Fluid ◽  
Charged Surface ◽  
Depolarization Current ◽  
Electrical Polarization ◽  
Thermally Stimulated Depolarization Current ◽  
Thermally Stimulated Depolarization ◽  
Calcium Phosphate Precipitation

We investigated the electrical polarizability of MgO and B2O3 containing bioactive glass (MBG). The MBG material with good manufacturing properties but low bioactivity was electrically polarized at a high dc field. The electrical polarizability of MBG was evaluated by thermally stimulated depolarization current (TSDC) measurements and immersion in simulated body fluid (SBF). The early precipitation of calcium phosphate on the negatively charged surface of the treated MBG demonstrated the increased bioactivity of the material and confirmed its polarizability. It is suggested that the electrical interactions between the polarized MBG and ions in SBF promoted the formation of the calcium phosphate precipitation. Accordingly, the increased bioactivity of the MBG in SBF is suggested to demonstrate the conversion of MBG into electrovector ceramics by the polarization treatment.

Control of Calcium Phosphate Precipitation in Hydrogel

2007 ◽  
pp. 79-82
Author(s):  
Masanobu Kamitakahara ◽  
Shinichi Ogata ◽  
Masao Tanihara ◽  
Chikara Ohtsuki
Keyword(s):  
Calcium Phosphate ◽  
Calcium Phosphate Precipitation

Effect of temperature and reactant concentration on calcium phosphate precipitation

2020 ◽  
Vol 552 ◽  
pp. 125909
Author(s):  
Luisa R. Brasil ◽  
Marcela C. Rodrigues ◽  
Thiago L.R. Hewer ◽  
Bruna M. Fronza ◽  
Victor E. Arana-Chavez ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Effect Of Temperature ◽  
Reactant Concentration ◽  
Calcium Phosphate Precipitation
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