Nucleation Kinetics of Calcium Phosphates on Polyelectrolyte Multilayers Displaying Internal Secondary Structure

2006 ◽  
Vol 6 (1) ◽  
pp. 327-334 ◽  
Author(s):  
Vincent Ball ◽  
Marc Michel ◽  
Fouzia Boulmedais ◽  
Joseph Hemmerle ◽  
Youssef Haikel ◽  
...  
Foods ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 998
Author(s):  
Laetitia Théron ◽  
Aline Bonifacie ◽  
Jérémy Delabre ◽  
Thierry Sayd ◽  
Laurent Aubry ◽  
...  

Food processing affects the structure and chemical state of proteins. In particular, protein oxidation occurs and may impair protein properties. These chemical reactions initiated during processing can develop during digestion. Indeed, the physicochemical conditions of the stomach (oxygen pressure, low pH) favor oxidation. In that respect, digestive proteases may be affected as well. Yet, very little is known about the link between endogenous oxidation of digestive enzymes, their potential denaturation, and, therefore, food protein digestibility. Thus, the objective of this study is to understand how oxidative chemical processes will impact the pepsin secondary structure and its hydrolytic activity. The folding and unfolding kinetics of pepsin under oxidative conditions was determined using Synchrotron Radiation Circular Dichroism. SRCD gave us the possibility to monitor the rapid kinetics of protein folding and unfolding in real-time, giving highly resolved spectral data. The proteolytic activity of control and oxidized pepsin was investigated by MALDI-TOF mass spectrometry on a meat protein model, the creatine kinase. MALDI-TOF MS allowed a rapid evaluation of the proteolytic activity through peptide fingerprint. This study opens up new perspectives by shifting the digestion paradigm taking into account the gastric digestive enzyme and its substrate.


Author(s):  
G Nicotra ◽  
S Lombardo ◽  
R Puglisi ◽  
C Spinella ◽  
G Ammendola ◽  
...  

2016 ◽  
Vol 441 ◽  
pp. 117-123 ◽  
Author(s):  
Guangchao Du ◽  
Zhaohui Sun ◽  
Yong Xian ◽  
Han Jing ◽  
Haijun Chen ◽  
...  

The model considered in part I is generalized to include growth mechanisms in which the chemical reaction which proceeds at the particle-atm osphere interface is reversible, so that molecules may evaporate from a particle as well as condense upon it. The Becker-Döring-Zeldovich-Frenkel theory of homogeneous nucleation kinetics is then reviewed in the light of the known statistical problem of the birth -and -death process, and an improved approximation is introduced which significantly alters the calculated results. Both steady-state nucleation kinetics and the time lag problem are discussed.


2021 ◽  
Vol 73 ◽  
pp. 165-170
Author(s):  
Zhipeng Long ◽  
Qiuyue Jiang ◽  
Jiantao Wang ◽  
Long Hou ◽  
Xing Yu ◽  
...  

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