Rapid Identification of Immunostimulatory α-Galactosylceramides Using Synthetic Combinatorial Libraries

2007 ◽  
Vol 9 (6) ◽  
pp. 1084-1093 ◽  
Author(s):  
Qian Li ◽  
Rachel M. Ndonye ◽  
Petr A. Illarionov ◽  
Karl O. A. Yu ◽  
Elliot S. Jerud ◽  
...  
Keyword(s):  
Combinatorial Libraries ◽  
Rapid Identification ◽  
Synthetic Combinatorial Libraries

Library of libraries: A novel approach in synthetic combinatorial libraries

Peptides ◽  
1995 ◽  
pp. 271-272
Author(s):  
Nikolai F. Sepetov ◽  
Viktor Krchňák ◽  
Magda Staňková ◽  
Kit S. Lam ◽  
Shelly Wade ◽  
...  
Keyword(s):  
Combinatorial Libraries ◽  
Novel Approach ◽  
Synthetic Combinatorial Libraries

scholarly journals Enhanced immune activity of cytotoxic T-lymphocyte epitope analogs derived from positional scanning synthetic combinatorial libraries

Blood ◽  
2001 ◽  
Vol 97 (6) ◽  
pp. 1776-1786 ◽  
Author(s):  
Corinna La Rosa ◽  
Radhika Krishnan ◽  
Susan Markel ◽  
Jonathan P. Schneck ◽  
Richard Houghten ◽  
...  
Keyword(s):  
T Cell ◽  
T Lymphocyte ◽  
Cytotoxic T Lymphocyte ◽  
Combinatorial Libraries ◽  
Peptide Sequence ◽  
Ctl Epitopes ◽  
Ctl Epitope ◽  
Positional Scanning ◽  
Synthetic Combinatorial Libraries

The pp65495-503 cytotoxic T-lymphocyte (CTL) epitope from cytomegalovirus (CMV) is universally recognized among CMV+ individuals who express an allele of the human leukocyte antigen A (HLA-A*0201). The relative binding affinity of the epitope to HLA-A*0201 is moderate, and its increased activity might prove beneficial in its use as a CTL epitope vaccine. A new approach to enhance the activity of T-cell epitopes is the use of positional scanning synthetic combinatorial libraries (PS-SCLs). Using a nonamer PS-SCL, the pp65495-503 epitope was modified after screening a CMV-specific T-cell clone (TCC) (3-3F4) from which the native peptide sequence was derived. Two peptides with amino acid substitutions at P1, P3, P7, and P8 are between 103 and 104 more active than the native epitope. Although the native CTL epitope terminates as a free acid, both tetrasubstituted peptides only function as CTL epitopes when the carboxyl terminus is amidated. Selective substitution of the native sequence based on PS-SCL screening results identified 3 amidated monosubstituted and disubstituted peptides that are better recognized than the native epitope by TCCs from a cohort expressing HLA-A*0201. In vitro stimulation of peripheral blood mononuclear cells with each of the peptide epitope analogs stimulated memory CTLs, which recognized CMV-infected targets among a high percentage of CMV+ individuals. Binding studies of peptide analogs with HLA-Ig (immunoglobulin) dimers and 2 different TCCs correlated with in vitro lysis results. These data suggest that increasing the activity of CTL epitopes while maintaining broad recognition is possible, which holds promise for vaccine development in infectious disease and cancer.

Findings on T cell specificity revealed by synthetic combinatorial libraries

2002 ◽  
Vol 267 (1) ◽  
pp. 79-97 ◽  
Author(s):  
Eva Borràs ◽  
Roland Martin ◽  
Valeria Judkowski ◽  
Jacqueline Shukaliak ◽  
Yingdong Zhao ◽  
...  
Keyword(s):  
T Cell ◽  
Combinatorial Libraries ◽  
Cell Specificity ◽  
Synthetic Combinatorial Libraries

ChemInform Abstract: Mixture-Based Synthetic Combinatorial Libraries

ChemInform ◽  
2010 ◽  
Vol 30 (49) ◽  
pp. no-no
Author(s):  
Richard A. Houghten ◽  
Clemencia Pinilla ◽  
Jon R. Appel ◽  
Sylvie E. Blondelle ◽  
Colette T. Dooley ◽  
...  
Keyword(s):  
Combinatorial Libraries ◽  
Synthetic Combinatorial Libraries

Strategies for the Use of Mixture-Based Synthetic Combinatorial Libraries: Scaffold Ranking, Direct Testing In Vivo, and Enhanced Deconvolution by Computational Methods

2008 ◽  
Vol 10 (1) ◽  
pp. 3-19 ◽  
Author(s):  
Richard A. Houghten ◽  
Clemencia Pinilla ◽  
Marc A. Giulianotti ◽  
Jon R. Appel ◽  
Colette T. Dooley ◽  
...  
Keyword(s):  
Computational Methods ◽  
Combinatorial Libraries ◽  
Direct Testing ◽  
Synthetic Combinatorial Libraries

scholarly journals Synthetic Peptide Combinatorial Libraries: A Method for the Identification of Bioactive Peptides Against Phytopathogenic Fungi

1997 ◽  
Vol 10 (5) ◽  
pp. 537-549 ◽  
Author(s):  
Jerry D. Reed ◽  
David L. Edwards ◽  
Carlos F. Gonzalez
Keyword(s):  
Mutagenic Activity ◽  
Fluorescent Microscopy ◽  
Combinatorial Libraries ◽  
Pythium Ultimum ◽  
Anastomosis Group ◽  
Phytopathogenic Fungi ◽  
Cytoplasmic Membranes ◽  
Synthetic Combinatorial Libraries ◽  
Spectrum Activity ◽  
Broad Spectrum Activity

Synthetic combinatorial libraries were evaluated with an iterative process to identify a hexapeptide with broad-spectrum activity against selected phytopathogenic fungi. A D-amino acid hexapeptide (FRLKFH) and pentapeptide (FRLHF) exhibited activity against Fusarium oxysporum f. sp. lycopersici, Rhizoctonia solani (anastomosis group 1), Ceratocystis fagacearum, and Pythium ultimum. The peptides showed no hemolytic or mutagenic activity. Fluorescent microscopy studies with a membrane impermeant dye indicated that fungal cytoplasmic membranes were compromised rapidly and that the nuclear membrane was also affected.

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