Directed Development of High-Performance Membranes via High-Throughput and Combinatorial Strategies

2006 ◽  
Vol 8 (2) ◽  
pp. 168-173 ◽  
Author(s):  
Metin Bulut ◽  
Lieven E. M. Gevers ◽  
Johan S. Paul ◽  
Ivo F. J. Vankelecom ◽  
Pierre A. Jacobs
Keyword(s):  
High Throughput ◽  
High Performance ◽  
Directed Development

scholarly journals Novel Electrochemiluminescent Assays for Drug Discovery

2000 ◽  
Vol 5 (1) ◽  
pp. 45-48 ◽  
Author(s):  
Maura C. Kibbey ◽  
David MacAllan ◽  
James W. Karaszkiewicz
Keyword(s):  
Drug Discovery ◽  
High Precision ◽  
High Throughput ◽  
High Performance ◽  
Dynamic Range ◽  
Wide Dynamic Range ◽  
Receptor Ligand ◽  
Enzymatic Assays ◽  
Detection Systems ◽  
Protein Assays

IGEN's ORIGEN® technology, which is based on electrochemiluminescence, has been adopted by a number of research and bioanalytical laboratories who have recognized its exquisite sensitivity, high precision, wide dynamic range, and flexibility in formatting a wide variety of applications. IGEN's M-SERIES™ marks the introduction of the second generation of detection systems employing the ORIGEN technology specifically repackaged to address the needs of the high throughput laboratories involved in drug discovery. Assays are formatted without wash steps. Users realize the high performance of a heterogeneous technology with the convenience of a homogeneous format. The M-SERIES platform can address enzymatic assays (kinases, proteases, helicases, etc.), receptor-ligand or protein-protein assays, immunoassays, quantitation of nucleic acids, as well as other applications. Recent assay formats will be explored in detail.

scholarly journals Introduction to Genomic Analysis Workshop: A catalyst for engaging life-science researchers in high throughput analysis

F1000Research ◽  
2019 ◽  
Vol 8 ◽  
pp. 1221 ◽  
Author(s):  
Phillip A. Richmond ◽  
Wyeth W. Wasserman
Keyword(s):  
High Throughput ◽  
High Performance ◽  
Life Science ◽  
Genomic Analysis ◽  
High Throughput Analysis ◽  
Time Period ◽  
Research Organizations ◽  
Efficacy Measures ◽  
Training Resources ◽  
Short Time

Researchers in the life sciences are increasingly faced with the task of obtaining compute resources and training to analyze large, high-throughput technology generated datasets. As demand for compute resources has grown, high performance computing (HPC) systems have been implemented by research organizations and international consortiums to support academic researchers. However, life science researchers lack effective time-of-need training resources for utilization of these systems. Current training options have drawbacks that inhibit the effective training of researchers without experience in computational analysis. We identified the need for flexible, centrally-organized, easily accessible, interactive, and compute resource specific training for academic HPC use.  In our delivery of a modular workshop series, we provided foundational training to a group of researchers in a coordinated manner, allowing them to further pursue additional training and analysis on compute resources available to them. Efficacy measures indicate that the material was effectively delivered to a broad audience in a short time period, including both virtual and on-site students. The practical approach to catalyze academic HPC use is amenable to diverse systems worldwide.

scholarly journals High-Throughput Fragment Screening by Affinity LC-MS

2012 ◽  
Vol 18 (2) ◽  
pp. 160-171 ◽  
Author(s):  
Minh-Dao Duong-Thi ◽  
Maria Bergström ◽  
Tomas Fex ◽  
Roland Isaksson ◽  
Sten Ohlson
Keyword(s):  
High Throughput ◽  
High Performance ◽  
Titration Calorimetry ◽  
Fragment Screening ◽  
Treatment Techniques ◽  
Alternative Approach ◽  
Fragment Library ◽  
Liquid Chromatography Separation ◽  
Pros And Cons ◽  
Resonance Surface

Fragment screening, an emerging approach for hit finding in drug discovery, has recently been proven effective by its first approved drug, vemurafenib, for cancer treatment. Techniques such as nuclear magnetic resonance, surface plasmon resonance, and isothemal titration calorimetry, with their own pros and cons, have been employed for screening fragment libraries. As an alternative approach, screening based on high-performance liquid chromatography separation has been developed. In this work, we present weak affinity LC/MS as a method to screen fragments under high-throughput conditions. Affinity-based capillary columns with immobilized thrombin were used to screen a collection of 590 compounds from a fragment library. The collection was divided into 11 mixtures (each containing 35 to 65 fragments) and screened by MS detection. The primary screening was performed in <4 h (corresponding to >3500 fragments per day). Thirty hits were defined, which subsequently entered a secondary screening using an active site-blocked thrombin column for confirmation of specificity. One hit showed selective binding to thrombin with an estimated dissociation constant ( KD) in the 0.1 mM range. This study shows that affinity LC/MS is characterized by high throughput, ease of operation, and low consumption of target and fragments, and therefore it promises to be a valuable method for fragment screening.

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