Synthesis and Screening of a Molecularly Imprinted Polymer Library Targeted for Penicillin G

2003 ◽  
Vol 5 (1) ◽  
pp. 67-72 ◽  
Author(s):  
Josefine Cederfur ◽  
Yuxin Pei ◽  
Meng Zihui ◽  
Maria Kempe
Keyword(s):  
Molecularly Imprinted Polymer ◽  
Penicillin G ◽  
Imprinted Polymer ◽  
Molecularly Imprinted

Development of Molecularly Imprinted Polymer‐Based Optical Sensor for the Sensitive Penicillin G Detection in Milk

2021 ◽  
Vol 6 (43) ◽  
pp. 11865-11875
Author(s):  
Volkan Safran ◽  
Ilgım Göktürk ◽  
Monireh Bakhshpour ◽  
Fatma Yılmaz ◽  
Adil Denizli
Keyword(s):  
Molecularly Imprinted Polymer ◽  
Optical Sensor ◽  
Penicillin G ◽  
Imprinted Polymer ◽  
Molecularly Imprinted

scholarly journals Selective Detection of Penicillin G Antibiotic in Milk by Molecularly Imprinted Polymer-Based Plasmonic SPR Sensor

Biomimetics ◽  
2021 ◽  
Vol 6 (4) ◽  
pp. 72
Author(s):  
Monireh Bakhshpour ◽  
Ilgım Göktürk ◽  
Nilay Bereli ◽  
Fatma Yılmaz ◽  
Adil Denizli
Keyword(s):  
Silver Nanoparticles ◽  
Surface Plasmon Resonance ◽  
Methyl Ester ◽  
Surface Plasmon ◽  
Molecularly Imprinted Polymer ◽  
Plasmon Resonance ◽  
Penicillin G ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Spr Sensor

Molecularly imprinted polymer-based surface plasmon resonance sensor prepared using silver nanoparticles was designed for the selective recognition of Penicillin G (PEN-G) antibiotic from both aqueous solution and milk sample. PEN-G imprinted sensors (NpMIPs) SPR sensor was fabricated using poly (2-hydroxyethyl methacrylate-N-methacroyl-(L)-cysteine methyl ester)-silver nanoparticles-N-methacryloyl-L-phenylalanine methyl ester polymer by embedding silver nanoparticles (AgNPs) into the polymeric film structure. In addition, a non-imprinted (NpNIPs) SPR sensor was prepared by utilizing the same polymerization recipe without addition of the PEN-G template molecule to evaluate the imprinting effect. FTIR-ATR spectrophotometer, ellipsometer, contact angle measurements were used for the characterization of NpMIPs SPR sensors. The linear concentration range of 0.01–10 ng/mL PEN-G was studied for kinetic analyses. The augmenting effect of AgNPs used to increase the surface plasmon resonance signal response was examined using polymer-based PEN-G imprinted (MIPs) sensor without the addition of AgNPs. The antibiotic amount present in milk chosen as a real sample was measured by spiking PEN-G into the milk. According to the Scatchard, Langmuir, Freundlich and Langmuir–Freundlich adsorption models, the interaction mechanism was estimated to be compatible with the Langmuir model.

Liquid Chromatography Analysis of Clozapine in Rat Brain Tissue, Using its Molecularly Imprinted Polymer after Administration of Toxic Dose of Drug and Lipid Emulsion Therapy

2019 ◽  
Vol 15 (3) ◽  
pp. 251-257
Author(s):  
Bahareh Sadat Yousefsani ◽  
Seyed Ahmad Mohajeri ◽  
Mohammad Moshiri ◽  
Hossein Hosseinzadeh
Keyword(s):  
Rat Brain ◽  
Brain Tissue ◽  
Molecularly Imprinted Polymer ◽  
Lipid Emulsion ◽  
Limit Of Detection ◽  
Imprinted Polymer ◽  
Toxic Dose ◽  
Molecularly Imprinted ◽  
The Brain ◽  
Rat Brain Tissue

Background:Molecularly imprinted polymers (MIPs) are synthetic polymers that have a selective site for a given analyte, or a group of structurally related compounds, that make them ideal polymers to be used in separation processes.Objective:An optimized molecularly imprinted polymer was selected and applied for selective extraction and analysis of clozapine in rat brain tissue.Methods:A molecularly imprinted solid-phase extraction (MISPE) method was developed for preconcentration and cleanup of clozapine in rat brain samples before HPLC-UV analysis. The extraction and analytical process was calibrated in the range of 0.025-100 ppm. Clozapine recovery in this MISPE process was calculated between 99.40 and 102.96%. The limit of detection (LOD) and the limit of quantification (LOQ) of the assay were 0.003 and 0.025 ppm, respectively. Intra-day precision values for clozapine concentrations of 0.125 and 0.025 ppm were 5.30 and 3.55%, whereas inter-day precision values of these concentrations were 9.23 and 6.15%, respectively. In this study, the effect of lipid emulsion infusion in reducing the brain concentration of drug was also evaluated.Results:The data indicated that calibrated method was successfully applied for the analysis of clozapine in the real rat brain samples after administration of a toxic dose to animal. Finally, the efficacy of lipid emulsion therapy in reducing the brain tissue concentration of clozapine after toxic administration of drug was determined.Conclusion:The proposed MISPE method could be applied in the extraction and preconcentration before HPLC-UV analysis of clozapine in rat brain tissue.

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