scholarly journals Structural Basis for Binding Specificity between Subclasses of Modular Polyketide Synthase Docking Domains

2009 ◽  
Vol 4 (1) ◽  
pp. 41-52 ◽  
Author(s):  
Tonia J. Buchholz ◽  
Todd W. Geders ◽  
Frank E. Bartley ◽  
Kevin A. Reynolds ◽  
Janet L. Smith ◽  
...  
Keyword(s):  
Polyketide Synthase ◽  
Binding Specificity ◽  
Structural Basis ◽  
Modular Polyketide Synthase

Promiscuity of a modular polyketide synthase towards natural and non-natural extender units

2013 ◽  
Vol 11 (27) ◽  
pp. 4449 ◽  
Author(s):  
Irina Koryakina ◽  
John B. McArthur ◽  
Matthew M. Draelos ◽  
Gavin J. Williams
Keyword(s):  
Polyketide Synthase ◽  
Modular Polyketide Synthase

scholarly journals Type II thioesterase ScoT is required for coelimycin production by the modular polyketide synthase Cpk of Streptomyces coelicolor A3(2).

2014 ◽  
Vol 61 (1) ◽  
Author(s):  
Magdalena Kotowska ◽  
Jarosław Ciekot ◽  
Krzysztof Pawlik
Keyword(s):  
Culture Medium ◽  
Polyketide Synthase ◽  
Streptomyces Coelicolor ◽  
Acyl Chain ◽  
No Production ◽  
Type I ◽  
Type Ii ◽  
Peptide Synthetases ◽  
Modular Polyketide Synthase ◽  
Editing Enzyme

Type II thioesterases were shown to maintain efficiency of modular type I polyketide synthases and nonribosomal peptide synthetases by removing acyl residues blocking extension modules. We found that thioesterase ScoT from Streptomyces coelicolor A3(2) is required for the production of the yellow-pigmented coelimycin by the modular polyketide synthase Cpk. No production of coelimycin was observed in cultures of scoT disruption mutant. Polyketide production was restored upon complementation with an intact copy of the scoT gene. An enzymatic assay showed that ScoT thioesterase can hydrolyse a 12-carbon acyl chain but the activity is too low to play a role in product release from the polyketide synthase. We conclude that ScoT is an editing enzyme necessary to maintain the activity of polyketide synthase Cpk. We provide a HPLC based method to measure the amount of coelimycin P2 in a culture medium.

Probing the Flexibility of an Iterative Modular Polyketide Synthase with Non-Native Substrates in Vitro

2018 ◽  
Vol 13 (8) ◽  
pp. 2261-2268 ◽  
Author(s):  
Samuel C. Curran ◽  
Andrew Hagen ◽  
Sean Poust ◽  
Leanne Jade G. Chan ◽  
Brett M. Garabedian ◽  
...  
Keyword(s):  
Polyketide Synthase ◽  
Modular Polyketide Synthase

Insights into the stereospecificity of ketoreduction in a modular polyketide synthase

2011 ◽  
Vol 9 (7) ◽  
pp. 2053 ◽  
Author(s):  
David H. Kwan ◽  
Manuela Tosin ◽  
Nadin Schläger ◽  
Frank Schulz ◽  
Peter F. Leadlay
Keyword(s):  
Polyketide Synthase ◽  
Modular Polyketide Synthase

scholarly journals Structural basis for the dual U4 and U4atac snRNA-binding specificity of spliceosomal protein hPrp31

RNA ◽  
2011 ◽  
Vol 17 (9) ◽  
pp. 1655-1663 ◽  
Author(s):  
S. Liu ◽  
H. Ghalei ◽  
R. Luhrmann ◽  
M. C. Wahl
Keyword(s):  
Binding Specificity ◽  
Structural Basis ◽  
Spliceosomal Protein

scholarly journals Molecular basis of celmer's rules: the role of two ketoreductase domains in the control of chirality by the erythromycin modular polyketide synthase

1999 ◽  
Vol 6 (4) ◽  
pp. 189-195 ◽  
Author(s):  
Inès E Holzbaur ◽  
Rebecca C Harris ◽  
Matthew Bycroft ◽  
Jesus Cortes ◽  
Christian Bisang ◽  
...  
Keyword(s):  
Molecular Basis ◽  
Polyketide Synthase ◽  
Modular Polyketide Synthase
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