Rapid Transfer of Transmembrane Proteins for Single Molecule Dimerization Assays in Polymer-Supported Membranes

2014 ◽  
Vol 9 (11) ◽  
pp. 2479-2484 ◽  
Author(s):  
Friedrich Roder ◽  
Stephan Wilmes ◽  
Christian P. Richter ◽  
Jacob Piehler
Keyword(s):  
Single Molecule ◽  
Transmembrane Proteins ◽  
Supported Membranes ◽  
Polymer Supported ◽  
Rapid Transfer

Reconstitution of Membrane Proteins into Polymer-Supported Membranes for Probing Diffusion and Interactions by Single Molecule Techniques

2011 ◽  
Vol 83 (17) ◽  
pp. 6792-6799 ◽  
Author(s):  
Friedrich Roder ◽  
Sharon Waichman ◽  
Dirk Paterok ◽  
Robin Schubert ◽  
Christian Richter ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Single Molecule ◽  
Supported Membranes ◽  
Polymer Supported

scholarly journals Polymer-Supported Membranes for Probing Transmembrane Protein Diffusion and Interaction by Single-Molecule Techniques

2011 ◽  
Vol 100 (3) ◽  
pp. 257a
Author(s):  
Friedrich Roder ◽  
Dirk Paterok ◽  
Sharon Waichman ◽  
Oliver Beutel ◽  
Jacob Piehler
Keyword(s):  
Single Molecule ◽  
Transmembrane Protein ◽  
Protein Diffusion ◽  
Supported Membranes ◽  
Polymer Supported

scholarly journals TRPV channel OCR-2 is distributed along C. elegans chemosensory cilia by diffusion in a local interplay with intraflagellar transport

2020 ◽  
Author(s):  
Jaap van Krugten ◽  
Noémie Danné ◽  
Erwin J.G. Peterman
Keyword(s):  
Single Molecule ◽  
Transmembrane Proteins ◽  
Intraflagellar Transport ◽  
Single Molecule Tracking ◽  
C Elegans ◽  
Normal Diffusion ◽  
Cellular Signal ◽  
Underlying Mechanisms ◽  
And Function

AbstractSensing and reacting to the environment is essential for survival and procreation of most organisms. Caenorhabditis elegans senses soluble chemicals with transmembrane proteins (TPs) in the cilia of its chemosensory neurons. Development, maintenance and function of these cilia relies on intraflagellar transport (IFT), in which motor proteins transport cargo, including sensory TPs, back and forth along the ciliary axoneme. Here we use live fluorescence imaging to show that IFT machinery and the sensory TP OCR-2 reversibly redistribute along the cilium after exposure to repellant chemicals. To elucidate the underlying mechanisms, we performed single-molecule tracking experiments and found that OCR-2 distribution depends on an intricate interplay between IFT-driven transport, normal diffusion and subdiffusion that depends on the specific location in the cilium. These insights in the role of IFT on the dynamics of cellular signal transduction contribute to a deeper understanding of the regulation of sensory TPs and chemosensing.

Spatial Organization of Lipid Phases in Micropatterned Polymer-Supported Membranes

2013 ◽  
Vol 135 (4) ◽  
pp. 1189-1192 ◽  
Author(s):  
Friedrich Roder ◽  
Oliver Birkholz ◽  
Oliver Beutel ◽  
Dirk Paterok ◽  
Jacob Piehler
Keyword(s):  
Spatial Organization ◽  
Supported Membranes ◽  
Polymer Supported

scholarly journals 1H0900 Regulation mechanism for the movement of transmembrane proteins and phospholipids : An approach by single molecule techniques

2002 ◽  
Vol 42 (supplement2) ◽  
pp. S41
Author(s):  
K. Murase ◽  
Y. Hirako ◽  
T. Fujiwara ◽  
R. Iino ◽  
K. Owaribe ◽  
...  
Keyword(s):  
Single Molecule ◽  
Transmembrane Proteins ◽  
Regulation Mechanism

PDNA as building blocks for membrane-guided self-assemblies

2007 ◽  
Vol 35 (3) ◽  
pp. 495-497 ◽  
Author(s):  
D. Pompon ◽  
A. Laisné
Keyword(s):  
Single Molecule ◽  
Self Assembly ◽  
Solid Phase ◽  
Metal Chelate ◽  
Cytochrome B5 ◽  
Iminodiacetic Acid ◽  
Building Blocks ◽  
Supported Membranes ◽  
Phase Synthesis ◽  
Synthesis Strategy

Different semi-synthetic PDNAs (protein–DNA complexes), which encompass a protein core engineered from the cytochrome b5 scaffold, an embedded tuneable redox cofactor, a synthetic linker and a large oligonucleotide, were designed, synthesized and purified to homogeneity. These building blocks can be reversibly attached to Ni-DOGS {1,2-dioleoyl-sn-glycero-3-[N(5-amino-1-carboxypentyl)iminodiacetic acid]succinyl}-doped supported membranes through a metal chelate bridge with the protein part and be polymerized in a fully controllable manner using a solid-phase synthesis strategy and a stepwise addition of suitable complementary oligonucleotides. The resulting structures could recreate a large range of regular distribution of patterned redox and absorbing centres separated by fully tuneable distances and geometry. Kinetic parameters for the self-assembly of building blocks were determined using SPRI (surface plasmon resonance imagery). Structures of resulting nano-objects were characterized using gel electrophoresis and single molecule approaches following decoration of assemblies with quantum dots.

Polymer-supported membranes

1984 ◽  
Vol 17 (6) ◽  
pp. 1292-1293 ◽  
Author(s):  
O. Albrecht ◽  
A. Laschewsky
Keyword(s):  
Supported Membranes ◽  
Polymer Supported

Polymer-Supported Membranes: Physical Models of Cell Surfaces

MRS Bulletin ◽  
2006 ◽  
Vol 31 (7) ◽  
pp. 513-520 ◽  
Author(s):  
Motomu Tanaka
Keyword(s):  
Materials Science ◽  
Inorganic Materials ◽  
Physical Models ◽  
Supported Membranes ◽  
Supported Membrane ◽  
Polymer Supports ◽  
Thin Polymer ◽  
Solid Substrates ◽  
Polymer Supported ◽  
Soft Biological Materials

The functional modification of solid surfaces with plasma membrane models has been drawing increasing attention as a straightforward strategy to bridge soft biological materials and hard inorganic materials. Planar model membranes can be deposited either directly on solid substrates (solid-supported membranes), or on ultrathin polymer supports (polymer-supported membranes) that mimic the generic role of the extracellular matrix and the cell surface. The first part of this review provides an overview of advances in the fabrication of polymer-supported membranes. The middle section describes how such thin polymer interlayers can physically modulate the membrane–substrate contact. The last section introduces several methods to localize membranes and membrane proteins. Finally, some ideas are presented on combining supported membrane concepts with semiconductor technology toward applications in materials science.

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