scholarly journals Small Molecules for Cell Reprogramming and Heart Repair: Progress and Perspective

2014 ◽  
Vol 9 (1) ◽  
pp. 34-44 ◽  
Author(s):  
Min Xie ◽  
Nan Cao ◽  
Sheng Ding
Keyword(s):  
Small Molecules ◽  
Cell Reprogramming ◽  
Heart Repair

Combining small molecules for cell reprogramming through an interatomic analysis

2013 ◽  
Vol 9 (11) ◽  
pp. 2741 ◽  
Author(s):  
Bruno César Feltes ◽  
Diego Bonatto
Keyword(s):  
Small Molecules ◽  
Cell Reprogramming

scholarly journals Novel weapon to conquer human glioblastoma: G-quadruplexes and neuro-inducers

2021 ◽  
Author(s):  
Galina Pavlova ◽  
Varvara Kolesnikova ◽  
Nadezhda Samoylenkova ◽  
Sergey Drozd ◽  
Alexander Revishchin ◽  
...  
Keyword(s):  
Small Molecules ◽  
Cell Cultures ◽  
Tumor Tissue ◽  
Therapy Resistance ◽  
Cell Reprogramming ◽  
Tumor Resistance ◽  
New Approach ◽  
Promising Strategy ◽  
Glioblastoma Recurrence ◽  
Different Response

Abstract Cancer cell reprogramming based on aptamers with antiproliferative properties in combination with small molecules that are used for conversion iPSCs into neurons represents a new approach to reduce the probability of glioblastoma recurrence and tumor resistance to therapy. In this research we tested several combinations of factors on whole cell cultures, derived from tumor tissue after surgical resection, and on cell cultures divided in CD133 enriched and depleted populations, as CD133 marker is believed to be characteristic for glioblastoma stem cells. We showed that CD133+ and CD133- cells have a different response to tested combinations of factors and CD133-positive cells are more stable and possess stemness properties. Thus, affecting these cells will lead to decrease of therapy resistance. Moreover, we found a combination of factors that is able to inhibit proliferation of both CD133+ and CD133- cells. Our results reveal a promising strategy to improve treatment of patients with glioblastoma.

A Revolution in Reprogramming: Small Molecules

2019 ◽  
Vol 19 (2) ◽  
pp. 77-90 ◽  
Author(s):  
Jin Zhou ◽  
Jie Sun
Keyword(s):  
Small Molecules ◽  
High Throughput Screening ◽  
Molecular Mechanisms ◽  
Genetic Factors ◽  
Cell Types ◽  
Cell Reprogramming ◽  
Cell Fates ◽  
Modern Biology ◽  
Reprogramming Efficiency

Transplantation of reprogrammed cells from accessible sources and in vivo reprogramming are potential therapies for regenerative medicine. During the last decade, genetic approaches, which mostly involved transcription factors and microRNAs, have been shown to affect cell fates. However, their potential carcinogenicity and other unexpected effects limit their translation into clinical applications. Recently, with the power of modern biology-oriented design and synthetic chemistry, as well as high-throughput screening technology, small molecules have been shown to enhance reprogramming efficiency, replace genetic factors, and help elucidate the molecular mechanisms underlying cellular plasticity and degenerative diseases. As a non-viral and non-integrating approach, small molecules not only show revolutionary capacities in generating desired exogenous cell types but also have potential as drugs that can restore tissues through repairing or reprogramming endogenous cells. Here, we focus on the recent progress made to use small molecules in cell reprogramming along with some related mechanisms to elucidate these issues.

scholarly journals Novel weapon to conquer human glioblastoma: G-quadruplexes and neuro-inducers

2021 ◽  
Author(s):  
Galina Pavlova ◽  
Varvara Kolesnikova ◽  
Nadezhda Samoylenkova ◽  
Sergey Drozd ◽  
Alexander Revishchin ◽  
...  
Keyword(s):  
Small Molecules ◽  
Cell Cultures ◽  
Tumor Tissue ◽  
Therapy Resistance ◽  
Cell Reprogramming ◽  
Tumor Resistance ◽  
New Approach ◽  
Promising Strategy ◽  
Glioblastoma Recurrence ◽  
Different Response

Abstract Cancer cell reprogramming based on aptamers with antiproliferative properties in combination with small molecules that are used for conversion iPSCs into neurons represents a new approach to reduce the probability of glioblastoma recurrence and tumor resistance to therapy. In this research we tested several combinations of factors on whole cell cultures, derived from tumor tissue after surgical resection, and on cell cultures divided in CD133 enriched and depleted populations, as CD133 marker is believed to be characteristic for glioblastoma stem cells. We showed that CD133+ and CD133- cells have a different response to tested combinations of factors and CD133-positive cells are more stable and possess stemness properties. Thus, affecting these cells will lead to decrease of therapy resistance. Moreover, we found a combination of factors that is able to inhibit proliferation of both CD133+ and CD133- cells. Our results reveal a promising strategy to improve treatment of patients with glioblastoma.

scholarly journals The use of small molecules in somatic-cell reprogramming

2014 ◽  
Vol 24 (3) ◽  
pp. 179-187 ◽  
Author(s):  
Alexander J. Federation ◽  
James E. Bradner ◽  
Alexander Meissner
Keyword(s):  
Small Molecules ◽  
Somatic Cell ◽  
Cell Reprogramming ◽  
Somatic Cell Reprogramming

scholarly journals Small molecules for cell reprogramming: a systems biology analysis

Aging ◽  
2021 ◽  
Author(s):  
Anna Knyazer ◽  
Gabriela Bunu ◽  
Dmitri Toren ◽  
Teodora Bucaciuc Mracica ◽  
Yael Segev ◽  
...  
Keyword(s):  
Systems Biology ◽  
Small Molecules ◽  
Cell Reprogramming

scholarly journals An Efficient Chemical-Defined Condition for Generation of Human Induced Pluripotent Stem Cells

2021 ◽  
Author(s):  
Xu Jinhong ◽  
Fang Shi ◽  
Wang Naweng ◽  
Li Bo ◽  
Huang Yongheng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factor ◽  
Small Molecules ◽  
Somatic Cell ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Cell Reprogramming ◽  
Human Somatic Cell ◽  
Somatic Cell Reprogramming ◽  
Induced Pluripotent

Abstract Background: Human induced pluripotent stem cells (hiPSCs) hold great potential in disease modeling, drug screening and cell therapy. However, efficiency and costs of hiPSCs preparation still need to be improved.Methods: We screened the compounds that target signaling pathways, epigenetic modifications or metabolic-process regulation to replace the growth factors. After small molecules treatment, TRA-1-60 staining was performed to quantify the efficiency of somatic cell reprogramming. Next, small molecule cocktail induced ESCs or iPSCs were examined with pluripotent markers expression. Finally, Genome-wide gene expression profile was then analyzed by RNA-seq to illustrate the mechanism of human somatic cell reprogramming. Result: Here, we found that a dual-specificity tyrosine phosphorylation-regulated kinase inhibitor ID-8 robustly enhanced human somatic cell reprogramming by upregulation of PDK4 and activation of glycolysis. Furthermore, we identified a novel growth-factor-free hiPSC generation system using small molecules ID-8/Kartogenin (IK). Finally, we developed IK medium combined with Low-dose bFGF to support the long-term expansion of human pluripotent stem cells. IK-iPSCs showed pluripotency and normal karyotype. Conclusions: Our studies may provide a novel growth-factor-free culture system to facilitate the generation of hiPSCs for multiple application in regenerative medicine.

Sign in / Sign up

Export Citation Format

Share Document