Determination of Protein–Ligand Binding Constants of a Cooperatively Regulated Tetrameric Enzyme Using Electrospray Mass Spectrometry

2013 ◽  
Vol 9 (1) ◽  
pp. 218-226 ◽  
Author(s):  
Dragana Cubrilovic ◽  
Wolfgang Haap ◽  
Konstantin Barylyuk ◽  
Armin Ruf ◽  
Martin Badertscher ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Ligand Binding ◽  
Electrospray Mass Spectrometry ◽  
Binding Constants

Determination of the endogenous phosphorylation state of B-50/GAP-43 and neurogranin in different brain regions by electrospray mass spectrometry

FEBS Letters ◽  
1996 ◽  
Vol 389 (3) ◽  
pp. 309-313 ◽  
Author(s):  
Monica Di Luca ◽  
Lucia Pastorino ◽  
Vittorio Raverdino ◽  
Pierre N.E. De Graan ◽  
Antonio Caputi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Mass Spectrometry ◽  
Brain Regions ◽  
Phosphorylation State

Liquid chromatography–electrospray mass spectrometry multi-residue determination of pesticides in apples and pears

1999 ◽  
Vol 830 (1) ◽  
pp. 135-143 ◽  
Author(s):  
E Lacassie ◽  
M.-F Dreyfuss ◽  
J.L Daguet ◽  
M Vignaud ◽  
P Marquet ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Electrospray Mass Spectrometry ◽  
Residue Determination

Simultaneous determination of albendazole metabolites, praziquantel and its metabolite in plasma by high-performance liquid chromatography–electrospray mass spectrometry

2007 ◽  
Vol 44 (2) ◽  
pp. 558-563 ◽  
Author(s):  
Pierina Sueli Bonato ◽  
Anderson Rodrigo Moraes de Oliveira ◽  
Fernando José Malagueño de Santana ◽  
Bruno José Dumet Fernandes ◽  
Vera Lucia Lanchote ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
High Performance ◽  
Electrospray Mass Spectrometry

Determination of Substrate/Ligand Binding Constants from Electromotrive Force Measurements

2008 ◽  
Vol 37 (4) ◽  
pp. 519-526 ◽  
Author(s):  
M. Lopez-Lopez ◽  
P. Lopez-Cornejo ◽  
A. García ◽  
F. Sanchez
Keyword(s):  
Ligand Binding ◽  
Binding Constants ◽  
Force Measurements

The determination of axial ligand binding constants for iron porphyrins by cyclic voltammetry

1991 ◽  
Vol 68 (4) ◽  
pp. 337 ◽  
Author(s):  
David K. Geiger ◽  
Edmund J. Paviak ◽  
Lawrence T. Kass
Keyword(s):  
Cyclic Voltammetry ◽  
Ligand Binding ◽  
Binding Constants ◽  
Axial Ligand ◽  
Iron Porphyrins

Determination of Solution Ions by Electrospray Mass Spectrometry

1994 ◽  
Vol 48 (6) ◽  
pp. 655-661 ◽  
Author(s):  
George R. Agnes ◽  
Gary Horlick
Keyword(s):  
Mass Spectrometry ◽  
Metal Ion ◽  
Gas Flow ◽  
Electrospray Mass Spectrometry ◽  
Negative Ion ◽  
Molecular Forms ◽  
Mass Spectral ◽  
Oxo Anions ◽  
Electrospray Source

Spectral results are presented illustrating the capability of electrospray mass spectrometry (ES-MS) for the determination of the composition of solution samples. The solution components that can be determined include the elemental and molecular forms of anions and cations. Three modes of positive-ion ES-MS are described: the ion cluster mode, the metal-ion mode, and an intermediate mode. The modes are established by operating the electrospray source at specific voltages and curtain gas flow rate values, and each mode provides a unique mass spectral picture of the solution components with primary focus on cationic species (i.e., metal ions). Results are also presented for operation of the electrospray source in negative-ion mode. In this mode both elemental anions (i.e., halides) and molecular anions (i.e., oxo-anions) can be determined.

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