Design and Kinetic Analysis of a Hybrid Promoter–Regulator System for Malonyl-CoA Sensing in Escherichia coli

2013 ◽  
Vol 9 (2) ◽  
pp. 451-458 ◽  
Author(s):  
Peng Xu ◽  
Wenya Wang ◽  
Lingyun Li ◽  
Namita Bhan ◽  
Fuming Zhang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Kinetic Analysis ◽  
Malonyl Coa ◽  
Hybrid Promoter

scholarly journals Characterization of the New AmpC β-Lactamase FOX-8 Reveals a Single Mutation, Phe313Leu, Located in the R2 Loop That Affects Ceftazidime Hydrolysis

2013 ◽  
Vol 57 (10) ◽  
pp. 5158-5161 ◽  
Author(s):  
Francisco José Pérez-Llarena ◽  
Frédéric Kerff ◽  
Laura Zamorano ◽  
María Carmen Fernández ◽  
Maria Luz Nuñez ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Kinetic Analysis ◽  
Clinical Strain ◽  
Single Mutation ◽  
Content Type ◽  
E Coli ◽  
Fold Reduction ◽  
Class C

ABSTRACTA novel class C β-lactamase (FOX-8) was isolated from a clinical strain ofEscherichia coli. The FOX-8 enzyme possessed a unique substitution (Phe313Leu) compared to FOX-3. IsogenicE. colistrains carrying FOX-8 showed an 8-fold reduction in resistance to ceftazidime relative to FOX-3. In a kinetic analysis, FOX-8 displayed a 33-fold reduction inkcat/Kmfor ceftazidime compared to FOX-3. In the FOX family of β-lactamases, the Phe313 residue located in the R2 loop affects ceftazidime hydrolysis and alters the phenotype ofE. colistrains carrying this variant.

Rapid Kinetic Analysis of Transcription Elongation by Escherichia coli RNA Polymerase

2008 ◽  
Vol 381 (5) ◽  
pp. 1106-1113 ◽  
Author(s):  
Ronald S. Johnson ◽  
Mark Strausbauch ◽  
Ryan Cooper ◽  
J. Kristen Register
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Kinetic Analysis ◽  
Transcription Elongation
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