scholarly journals Genetic Incorporation of Twelve meta-Substituted Phenylalanine Derivatives Using a Single Pyrrolysyl-tRNA Synthetase Mutant

2012 ◽  
Vol 8 (2) ◽  
pp. 405-415 ◽  
Author(s):  
Yane-Shih Wang ◽  
Xinqiang Fang ◽  
Hsueh-Ying Chen ◽  
Bo Wu ◽  
Zhiyong U. Wang ◽  
...  
Keyword(s):  
Trna Synthetase ◽  
Genetic Incorporation

scholarly journals Genetic Incorporation of Histidine Derivatives Using an Engineered Pyrrolysyl-tRNA Synthetase

2014 ◽  
Vol 9 (5) ◽  
pp. 1092-1096 ◽  
Author(s):  
Han Xiao ◽  
Francis B. Peters ◽  
Peng-Yu Yang ◽  
Sean Reed ◽  
Johnathan R. Chittuluru ◽  
...  
Keyword(s):  
Trna Synthetase ◽  
Histidine Derivatives ◽  
Genetic Incorporation

The de novo engineering of pyrrolysyl-tRNA synthetase for genetic incorporation of l-phenylalanine and its derivatives

2011 ◽  
Vol 7 (3) ◽  
pp. 714 ◽  
Author(s):  
Yane-Shih Wang ◽  
William K. Russell ◽  
Zhiyong Wang ◽  
Wei Wan ◽  
Lindsey E. Dodd ◽  
...  
Keyword(s):  
De Novo ◽  
Trna Synthetase ◽  
Genetic Incorporation

scholarly journals Genetic encoding of a highly photostable, long lifetime fluorescent amino acid for imaging in mammalian cells

2021 ◽  
Author(s):  
Chloe M. Jones ◽  
D. Miklos Robkis ◽  
Robert J. Blizzard ◽  
Mika Munari ◽  
Yarra Venkatesh ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Mammalian Cells ◽  
Trna Synthetase ◽  
Fluorescence Lifetime Imaging ◽  
High Quantum Yield ◽  
Lifetime Imaging ◽  
Genetic Encoding ◽  
Long Lifetime ◽  
Fluorescent Amino Acid ◽  
Genetic Incorporation

Acridonylalanine (Acd) is photostable, with a high quantum yield and long fluorescence lifetime in water. An evolved tRNA synthetase (RS) enables genetic incorporation of Acd in mammalian cells and its use in fluorescence lifetime imaging microscopy.

scholarly journals The genetic incorporation of thirteen novel non-canonical amino acids

2014 ◽  
Vol 50 (20) ◽  
pp. 2673-2675 ◽  
Author(s):  
Alfred Tuley ◽  
Yane-Shih Wang ◽  
Xinqiang Fang ◽  
Yadagiri Kurra ◽  
Yohannes H. Rezenom ◽  
...  
Keyword(s):  
Amino Acids ◽  
Trna Synthetase ◽  
Amber Codon ◽  
Genetic Incorporation

Thirteen novel non-canonical amino acids were synthesized and tested for suppression of an amber codon using a mutant pyrrolysyl-tRNA synthetase–tRNAPylCUA pair.

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