Role of SbmA in the Uptake of Peptide Nucleic Acid (PNA)-Peptide Conjugates in E. coli

2012 ◽  
Vol 8 (2) ◽  
pp. 360-367 ◽  
Author(s):  
Anubrata Ghosal ◽  
Ally Vitali ◽  
James E.M. Stach ◽  
Peter E. Nielsen
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Peptide Conjugates ◽  
E Coli

Correction to Role of SbmA in the Uptake of Peptide Nucleic Acid (PNA)-Peptide Conjugates in E. coli

2014 ◽  
Vol 10 (2) ◽  
pp. 631-631 ◽  
Author(s):  
Anubrata Ghosal ◽  
Ally Vitali ◽  
James E. M. Stach ◽  
Peter E. Nielsen
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Peptide Conjugates ◽  
E Coli

Enhanced Strand Invasion by Peptide Nucleic Acid−Peptide Conjugates†

Biochemistry ◽  
2002 ◽  
Vol 41 (37) ◽  
pp. 11118-11125 ◽  
Author(s):  
Kunihiro Kaihatsu ◽  
Dwaine A. Braasch ◽  
Ahmet Cansizoglu ◽  
David R. Corey
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Peptide Conjugates ◽  
Strand Invasion

scholarly journals Detection of Escherichia coli O157 by Peptide Nucleic Acid FluorescenceIn SituHybridization (PNA-FISH) and Comparison to a Standard Culture Method

2013 ◽  
Vol 79 (20) ◽  
pp. 6293-6300 ◽  
Author(s):  
C. Almeida ◽  
J. M. Sousa ◽  
R. Rocha ◽  
L. Cerqueira ◽  
S. Fanning ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Food Samples ◽  
High Morbidity ◽  
Content Type ◽  
E Coli ◽  
Fish Method ◽  
The World ◽  
Pna Fish

ABSTRACTDespite the emergence of non-O157 Shiga toxin-producingEscherichia coli(STEC) infections,E. coliserotype O157 is still the most commonly identified STEC in the world. It causes high morbidity and mortality and has been responsible for a number of outbreaks in many parts of the world. Various methods have been developed to detect this particular serotype, but standard bacteriological methods remain the gold standard. Here, we propose a new peptide nucleic acid fluorescencein situhybridization (PNA-FISH) method for the rapid detection ofE. coliO157. Testing on 54 representative strains showed that the PNA probe is highly sensitive and specific toE. coliO157. The method then was optimized for detection in food samples. Ground beef and unpasteurized milk samples were artificially contaminated withE. coliO157 concentrations ranging from 1 × 10−2to 1 × 102CFU per 25 g or ml of food. Samples were then preenriched and analyzed by both the traditional bacteriological method (ISO 16654:2001) and PNA-FISH. The PNA-FISH method performed well in both types of food matrices with a detection limit of 1 CFU/25 g or ml of food samples. Tests on 60 food samples have shown a specificity value of 100% (95% confidence interval [CI], 82.83 to 100), a sensitivity of 97.22% (95% CI, 83.79 to 99.85%), and an accuracy of 98.33% (CI 95%, 83.41 to 99.91%). Results indicate that PNA-FISH performed as well as the traditional culture methods and can reduce the diagnosis time to 1 day.

Triplex-forming PNA modified with unnatural nucleobases: the role of protonation entropy in RNA binding

2016 ◽  
Vol 18 (47) ◽  
pp. 32002-32006 ◽  
Author(s):  
Tamaki Endoh ◽  
Chiara Annoni ◽  
Dziyana Hnedzko ◽  
Eriks Rozners ◽  
Naoki Sugimoto
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Rna Binding ◽  
Association Rate

Protonation entropy of 2-aminopyridine is responsible for decreased association rate and stability of triplex between peptide nucleic acid and RNA at higher pH.

Role of Different Receptor-Surface Binding Modes in the Morphological and Electrochemical Properties of Peptide-Nucleic-Acid-Based Sensing Platforms

Langmuir ◽  
2019 ◽  
Vol 35 (9) ◽  
pp. 3272-3283 ◽  
Author(s):  
Johannes D. Bartl ◽  
Paolo Scarbolo ◽  
Denis Brandalise ◽  
Martin Stutzmann ◽  
Marc Tornow ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Electrochemical Properties ◽  
Peptide Nucleic Acid ◽  
Binding Modes ◽  
Surface Binding ◽  
Sensing Platforms

Role of Nucleobase Energetics and Nucleobase Interactions in Single-Stranded Peptide Nucleic Acid Charge Transfer

2009 ◽  
Vol 131 (18) ◽  
pp. 6498-6507 ◽  
Author(s):  
Amit Paul ◽  
Silvia Bezer ◽  
Ravindra Venkatramani ◽  
Laura Kocsis ◽  
Emil Wierzbinski ◽  
...  
Keyword(s):  
Charge Transfer ◽  
Nucleic Acid ◽  
Peptide Nucleic Acid
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