Fluorescence Lifetime of Fluorescent Proteins as an Intracellular Environment Probe Sensing the Cell Cycle Progression

2012 ◽  
Vol 7 (8) ◽  
pp. 1385-1392 ◽  
Author(s):  
Artem Pliss ◽  
Lingling Zhao ◽  
Tymish Y. Ohulchanskyy ◽  
Junle Qu ◽  
Paras N. Prasad
Keyword(s):  
Cell Cycle ◽  
Fluorescence Lifetime ◽  
Cell Cycle Progression ◽  
Fluorescent Proteins ◽  
Cycle Progression ◽  
Intracellular Environment

scholarly journals Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression

2020 ◽  
Author(s):  
Ryoko Ando ◽  
Asako Sakaue-Sawano ◽  
Keiko Shoda ◽  
Atsushi Miyawaki
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Progression ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Dimeric Complex ◽  
Cycle Progression ◽  
Multimeric Complex ◽  
Color Variant ◽  
Cellular Labeling ◽  
Cycle Indicator

We cloned and characterized two new coral fluorescent proteins: h2-3 and 1-41. h2-3 formed an obligate dimeric complex and exhibited bright fluorescence. On the other hand, 1-41 formed a highly multimeric complex and exhibited dim red fluorescence. We engineered 1-41 into AzaleaB5, a practically useful red-emitting fluorescent protein for cellular labeling applications. We fused h2-3 and AzaleaB5 to the ubiquitination domains of human Geminin and Cdt1, respectively, to generate a new color variant of Fucci (Fluorescent Ubiquitination-based Cell-Cycle Indicator): Fucci5. We found Fucci5 provided brighter nuclear labeling for monitoring cell cycle progression than the 1st and 2nd generations that used mAG/mKO2 and mVenus/mCherry, respectively.

1955-P: GLP-1R Activation Attenuates Prostate Cancer Growth via Inhibiting Cell Cycle Progression

Diabetes ◽  
2019 ◽  
Vol 68 (Supplement 1) ◽  
pp. 1955-P
Author(s):  
TORU SHIGEOKA ◽  
TAKASHI NOMIYAMA ◽  
TAKAKO KAWANAMI ◽  
YURIKO HAMAGUCHI ◽  
TOMOKO TANAKA ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cell Cycle ◽  
Cell Cycle Progression ◽  
Cancer Growth ◽  
Cycle Progression
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