scholarly journals Characterization of Molecular Interactions between ACP and Halogenase Domains in the Curacin A Polyketide Synthase

2011 ◽  
Vol 7 (2) ◽  
pp. 378-386 ◽  
Author(s):  
Alena Busche ◽  
Daniel Gottstein ◽  
Christopher Hein ◽  
Nina Ripin ◽  
Irina Pader ◽  
...  
Keyword(s):  
Molecular Interactions ◽  
Polyketide Synthase ◽  
Curacin A

Characterization of Molecular Interactions of Mytilus edulis Foot Proteins on Model Hydrated Surfaces

2001 ◽  
Author(s):  
Gill G. Geesey ◽  
Peter A. Suci ◽  
Peter R. Griffiths ◽  
Georges Belfort
Keyword(s):  
Mytilus Edulis ◽  
Molecular Interactions

scholarly journals Identification and characterization of molecular interactions between mortalin/mtHsp70 and HSP60

2005 ◽  
Vol 391 (2) ◽  
pp. 185-190 ◽  
Author(s):  
Renu Wadhwa ◽  
Syuichi Takano ◽  
Kamaljit Kaur ◽  
Satoshi Aida ◽  
Tomoko Yaguchi ◽  
...  
Keyword(s):  
Heat Shock ◽  
Molecular Interactions ◽  
Heat Shock Protein 60 ◽  
Hsp60 Expression ◽  
Mitochondrial Hsp70 ◽  
Shock Proteins ◽  
First Time

Mortalin/mtHsp70 (mitochondrial Hsp70) and HSP60 (heat-shock protein 60) are heat-shock proteins that reside in multiple subcellular compartments, with mitochondria being the predominant one. In the present study, we demonstrate that the two proteins interact both in vivo and in vitro, and that the N-terminal region of mortalin is involved in these interactions. Suppression of HSP60 expression by shRNA (short hairpin RNA) plasmids caused the growth arrest of cancer cells similar to that obtained by suppression of mortalin expression by ribozymes. An overexpression of mortalin, but not of HSP60, extended the in vitro lifespan of normal fibroblasts (TIG-1). Taken together, this study for the first time delineates: (i) molecular interactions of HSP60 with mortalin; (ii) their co- and exclusive localizations in vivo; (iii) their involvement in tumorigenesis; and (iv) their functional distinction in pathways involved in senescence.

Identification and characterization of the polyketide synthase involved in ochratoxin A biosynthesis in Aspergillus carbonarius

2014 ◽  
Vol 179 ◽  
pp. 10-17 ◽  
Author(s):  
Antonia Gallo ◽  
Benjamin P. Knox ◽  
Kenneth S. Bruno ◽  
Michele Solfrizzo ◽  
Scott E. Baker ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Polyketide Synthase ◽  
Aspergillus Carbonarius ◽  
Identification And Characterization

scholarly journals Interfacial characterization of the molecular interactions in mixed monolayers of coumarin and phospholipids

2019 ◽  
Vol 31 (4) ◽  
pp. 452-459 ◽  
Author(s):  
Felipe S. Rocha ◽  
Gustavo S. Codeceira ◽  
Maria D.L. Oliveira ◽  
Cesar A.S. Andrade
Keyword(s):  
Molecular Interactions ◽  
Mixed Monolayers

scholarly journals Characterization of distinct molecular interactions responsible for IRF3 and IRF7 phosphorylation and subsequent dimerization

2020 ◽  
Vol 48 (20) ◽  
pp. 11421-11433
Author(s):  
Louise Dalskov ◽  
Ryo Narita ◽  
Line L Andersen ◽  
Nanna Jensen ◽  
Sonia Assil ◽  
...  
Keyword(s):  
Immune Response ◽  
Transcription Factors ◽  
Molecular Interactions ◽  
Adaptor Proteins ◽  
Innate Immune ◽  
Charged Surface ◽  
Regulatory Domain ◽  
Phosphorylation Event ◽  
Positively Charged

Abstract IRF3 and IRF7 are critical transcription factors in the innate immune response. Their activation is controlled by phosphorylation events, leading to the formation of homodimers that are transcriptionally active. Phosphorylation occurs when IRF3 is recruited to adaptor proteins via a positively charged surface within the regulatory domain of IRF3. This positively charged surface also plays a crucial role in forming the active homodimer by interacting with the phosphorylated sites stabilizing the homodimer. Here, we describe a distinct molecular interaction that is responsible for adaptor docking and hence phosphorylation as well as a separate interaction responsible for the formation of active homodimer. We then demonstrate that IRF7 can be activated by both MAVS and STING in a manner highly similar to that of IRF3 but with one key difference. Regulation of IRF7 appears more tightly controlled; while a single phosphorylation event is sufficient to activate IRF3, at least two phosphorylation events are required for IRF7 activation.

scholarly journals Biochemical characterization of the minimal domains of an iterative eukaryotic polyketide synthase

FEBS Journal ◽  
2018 ◽  
Vol 285 (23) ◽  
pp. 4494-4511 ◽  
Author(s):  
Martin Sabatini ◽  
Santiago Comba ◽  
Silvia Altabe ◽  
Alejandro I. Recio‐Balsells ◽  
Guillermo R. Labadie ◽  
...  
Keyword(s):  
Polyketide Synthase ◽  
Biochemical Characterization

Characterization of Streptomyces argillaceus genes encoding a polyketide synthase involved in the biosynthesis of the antitumor mithramycin

Gene ◽  
1996 ◽  
Vol 172 (1) ◽  
pp. 87-91 ◽  
Author(s):  
Felipe Lombó ◽  
Gloria Blanco ◽  
Ernestina Fernández ◽  
Carmen Méndez ◽  
JoséA. Salas
Keyword(s):  
Polyketide Synthase ◽  
Genes Encoding

Functional characterization of a veA-dependent polyketide synthase gene in Aspergillus flavus necessary for the synthesis of asparasone, a sclerotium-specific pigment

2014 ◽  
Vol 64 ◽  
pp. 25-35 ◽  
Author(s):  
Jeffrey W. Cary ◽  
Pamela Y. Harris-Coward ◽  
Kenneth C. Ehrlich ◽  
José Diana Di Mavungu ◽  
Svetlana V. Malysheva ◽  
...  
Keyword(s):  
Aspergillus Flavus ◽  
Polyketide Synthase ◽  
Functional Characterization ◽  
Polyketide Synthase Gene

scholarly journals Genetic Localization and Molecular Characterization of the nonS Gene Required for Macrotetrolide Biosynthesis inStreptomyces griseus DSM40695

2000 ◽  
Vol 44 (7) ◽  
pp. 1809-1817 ◽  
Author(s):  
Wyatt C. Smith ◽  
Longkuan Xiang ◽  
Ben Shen
Keyword(s):  
Polyketide Synthase ◽  
Isotope Labeling ◽  
Streptomyces Griseus ◽  
Open Reading Frames ◽  
A Cell ◽  
Cyclic Polyethers ◽  
The Difference ◽  
Reading Frames

ABSTRACT The macrotetrolides are a family of cyclic polyethers derived from tetramerization, in a stereospecific fashion, of the enantiomeric nonactic acid (NA) and its homologs. Isotope labeling experiments established that NA is of polyketide origin, and biochemical investigations demonstrated that 2-methyl-6,8-dihydroxynon-2E-enoic acid can be converted into NA by a cell-free preparation from Streptomyces lividans that expresses nonS. These results lead to the hypothesis that macrotetrolide biosynthesis involves a pair of enantiospecific polyketide pathways. In this work, a 55-kb contiguous DNA region was cloned from Streptomyces griseus DSM40695, a 6.3-kb fragment of which was sequenced to reveal five open reading frames, including the previously reported nonR andnonS genes. Inactivation of nonS in vivo completely abolished macrotetrolide production. Complementation of thenonS mutant by the expression of nonS intrans fully restored its macrotetrolide production ability, with a distribution of individual macrotetrolides similar to that for the wild-type producer. In contrast, fermentation of thenonS mutant in the presence of exogenous (±)-NA resulted in the production of nonactin, monactin, and dinactin but not in the production of trinactin and tetranactin. These results prove the direct involvement of nonS in macrotetrolide biosynthesis. The difference in macrotetrolide production between in vivo complementation of the nonS mutant by the plasmid-borne nonSgene and fermentation of the nonS mutant in the presence of exogenously added (±)-NA suggests that NonS catalyzes the formation of (−)-NA and its homologs, supporting the existence of a pair of enantiospecific polyketide pathways for macrotetrolide biosynthesis inS. griseus. The latter should provide a model that can be used to study the mechanism by which polyketide synthase controls stereochemistry during polyketide biosynthesis.

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