Estimation of O2 and CO2 Solubility in Microbial Culture Media

1999 ◽  
Vol 15 (5) ◽  
pp. 923-927 ◽  
Author(s):  
J.B. Gros ◽  
C.G. Dussap ◽  
M. Catte
Keyword(s):  
Culture Media ◽  
Microbial Culture ◽  
Co2 Solubility

A gas phase chromatography method for determination of low dissolved CO2 concentration and/or CO2 solubility in microbial culture media

1995 ◽  
Vol 9 (11) ◽  
pp. 787-792 ◽  
Author(s):  
Anne Marty ◽  
Jean-Fran�ois Cornet ◽  
Gholamreza Djelveh ◽  
Christian Larroche ◽  
Jean-Bernard Gros
Keyword(s):  
Gas Phase ◽  
Culture Media ◽  
Co2 Concentration ◽  
Microbial Culture ◽  
Co2 Solubility ◽  
Chromatography Method

scholarly journals Determination of Dissolved CO2 Concentration in Culture Media: Evaluation of pH Value and Mathematical Data

Processes ◽  
2020 ◽  
Vol 8 (11) ◽  
pp. 1373
Author(s):  
Amir Izzuddin Adnan ◽  
Mei Yin Ong ◽  
Saifuddin Nomanbhay ◽  
Pau Loke Show
Keyword(s):  
Carbon Dioxide ◽  
Ph Value ◽  
Culture Media ◽  
Co2 Concentration ◽  
Co2 Solubility ◽  
Greenhouse Gasses ◽  
Dipotassium Phosphate ◽  
The Difference ◽  
Carbon Dioxide Co2

Carbon dioxide is the most influential gas in greenhouse gasses and its amount in the atmosphere reached 412 µmol/mol in August 2020, which increased rapidly, by 48%, from preindustrial levels. A brand-new chemical industry, namely organic chemistry and catalysis science, must be developed with carbon dioxide (CO2) as the source of carbon. Nowadays, many techniques are available for controlling and removing carbon dioxide in different chemical processes. Since the utilization of CO2 as feedstock for a chemical commodity is of relevance today, this study will focus on how to increase CO2 solubility in culture media used for growing microbes. In this work, the CO2 solubility in a different medium was investigated. Sodium hydroxide (NaOH) and monoethanolamine (MEA) were added to the culture media (3.0 g/L dipotassium phosphate (K2HPO4), 0.2 g/L magnesium chloride (MgCl2), 0.2 g/L calcium chloride (CaCl2), and 1.0 g/L sodium chloride (NaCl)) for growing microbes in order to observe the difference in CO2 solubility. Factors of temperature and pressure were also studied. The determination of CO2 concentration in the solution was measured by gas analyzer. The result obtained from optimization revealed a maximum CO2 concentration of 19.029 mol/L in the culture media with MEA, at a pressure of 136.728 kPa, operating at 20.483 °C.

Guar gum: a cheap substitute for agar in microbial culture media

2005 ◽  
Vol 41 (4) ◽  
pp. 345-349 ◽  
Author(s):  
R. Jain ◽  
V. Anjaiah ◽  
S.B. Babbar
Keyword(s):  
Guar Gum ◽  
Culture Media ◽  
Microbial Culture

Isubgol as an Alternative Gelling Agent for Microbial Culture Media

1997 ◽  
Vol 6 (2) ◽  
pp. 129-131 ◽  
Author(s):  
Neeru Jain ◽  
Shruti Gupta ◽  
Shashi B. Babbar
Keyword(s):  
Culture Media ◽  
Gelling Agent ◽  
Microbial Culture ◽  
Alternative Gelling Agent

Evaluation of guar gum derivatives as gelling agents for microbial culture media

2012 ◽  
Vol 28 (5) ◽  
pp. 2279-2285 ◽  
Author(s):  
Waikhom Gangotri ◽  
Ruchi Jain-Raina ◽  
Shashi B. Babbar
Keyword(s):  
Guar Gum ◽  
Culture Media ◽  
Microbial Culture ◽  
Gelling Agents

scholarly journals Semen bacterial contamination: Antibiotics susceptibility pattern and standard sperm parameters in men of Birjand, 2017

2020 ◽  
pp. 172-181
Keyword(s):  
Urinary Tract ◽  
Bacterial Contamination ◽  
Culture Media ◽  
Sperm Count ◽  
Semen Analysis ◽  
Sperm Parameters ◽  
Diffusion Method ◽  
Microbial Culture ◽  
Microbiological Methods ◽  
Tract Infections

Background and Aim: Urinary tract infections can be one of the most important causes of infertility in men. Identification of semen bacterial contamination and using of appropriate antibiotic treatment can cause to improvement sperm parameters. The aim of this study was to evaluate the frequency of bacterial contamination of semen and its effect on standard sperm parameters. Materials and Methods: In this descriptive-analytical study, the semen analysis of 40 men referring to Birjand laboratories was performed by using the CASA model spermogram device. The Eosin-Nigrosin staining was used to evaluate of sperm viability. After semen samples were cultured on microbial culture media, bacterial contamination was assessed by using conventional microbiological methods. The determination of antibiotic susceptibility of bacterial isolates was performed by the disk diffusion method. Results: In the present study, 30% of semen 12 samples were detected with bacterial contamination, the most frequent of which was related to Escherichia coli (41.7%). Statistical analysis showed that there was a significant relationship between bacterial contamination of semen and history of urinary tract infection (UTI) (P=0.001). There was a significant decrease in the motility, viability and sperm count in subjects with bacterial semen contamination (P<0.05). Nitrofurantoin, Gentamicin and third-generation of Cephalosporins were reported as the most effective treatment options for reducing bacterial contamination in semen. Conclusion: The bacterial contamination of semen can significantly reduce the motility, viability and sperm count. Due to the high prevalence of bacterial contamination in semen and its significant association with sperm factors, the microbial screening of infertile couples without clinical symptoms is essential. Key Words: Antibiotic Resistance; Bacterial Contamination; Infertility; Semen fluid; Sperm

scholarly journals Evaluation OfProsopis Africana Seed Hydrolysates As Source Of Peptone For Microbial Culture Media

2017 ◽  
Vol 16 (05) ◽  
pp. 36-47
Author(s):  
Onah, AI ◽  
Okore, VC ◽  
Tony Onah, OF ◽  
Uronnachi, EM
Keyword(s):  
Culture Media ◽  
Microbial Culture

scholarly journals EKSTRAKSI RUMPUT LAUT Gelidium sp. MENJADI BAKTO AGAR SEBAGAI PEMADAT MEDIA PERTUMBUHAN MIKROBA

2019 ◽  
Vol 9 (2) ◽  
pp. 71
Author(s):  
Zachra Resha Shantika ◽  
Srikandi Srikandi ◽  
RTM Sutamihardja
Keyword(s):  
Trace Elements ◽  
Bacillus Subtilis ◽  
Culture Media ◽  
Economic Value ◽  
Growth Media ◽  
Microbial Culture ◽  
Metal Levels ◽  
Bacterial Morphology ◽  
Microbiological Testing ◽  
Agar Media

Extraction of Seaweed Grass Gelidium Sp. Become A Bakto For A Microbic Growth Media Competition      Red seaweed is seaweed that produces gelatin one example is Gelidium sp. The product that has high economic value is bakto agar which is widely used in the field of microbiology, namely as a microbial culture media compactor. The use of bacto so that it will increase domestically, so as to meet the needs of bakto so that in the country with the expected quality classification with bakto so that the commercial is carried out this research. Agar yield value of seaweed type Gelidium sp. produced an average of 26.69%. The proximate test results for the parameters of water content, levels of ash, insoluble ash and sulfate in bakto so that the research is 6.89%, 1.67%, 0.0235%, and 1.31% respectively. The results of testing the levels of protein, fat, carbohydrate and gel strength at bacto so that respectively are 1.72%, 0.05%, 32.87% and 214.45 (g / cm2). The results of testing the mineral metal levels of calcium, magnesium and potassium were obtained at bakto so that they were 1806.06 mg / kg, 680 mg / kg and 338.22 mg / kg respectively. The results of testing the levels of trace elements of iron and copper were obtained at 8.75 mg / kg and 2.10 mg / kg. The results of microbiological testing using the total plate number method using Bacillus subtilis and Eschericia coli bacteria were found to be 8.6 x 106 colonies / mL and 2.81 x 107 colonies / mL. Based on the observation of bacterial morphology by comparing it with bakto so that the commercial standard in general is not much different. So based on the results of proximate and microbiological testing, bakto so that this research can be used as a medium for bacterial growth.Keywords: Gelidium sp., Agar, media and bacteriaABSTRAK      Rumput laut merah merupakan rumput laut yang menghasilkan agar-agar salah satu contohnya adalah Gelidium sp. Produk agar yang memiliki nilai ekonomis tinggi adalah bakto agar yang banyak digunakan dalam bidang mikrobiologi yaitu sebagai pemadat media kultur mikroorganisme. Pemanfaatan bakto agar di dalam negeri semakin meningkat, sehingga untuk mencukupi kebutuhan bakto agar di dalam negeri dengan klasifikasi mutu yang diharapkan sama dengan bakto agar komersial maka dilakukan penelitian ini. Nilai rendemen agar dari rumput laut jenis Gelidium sp. dihasilkan rata-rata sebesar 26.69%. Hasil pengujian proksimat untuk parameter kadar air, kadar abu, abu tak larut asam dan sulfat pada bakto agar penelitian ini berturut-turut adalah sebesar 6,89%, 1,67%, 0,0235%, dan 1.31%. Hasil pengujian kadar protein, lemak, karbohidrat dan kekuatan gel pada bakto agar berturut-turut adalah sebesar 1,72%, 0,05%, 32,87% dan 214,45 (g/cm2). Hasil pengujian kadar logam mineral kalsium, magnesium dan kalium didapatkan pada bakto agar berturut-turut adalah sebesar 1806,06 mg/kg, 680 mg/kg dan 338,22 mg/kg. Hasil pengujian kadar logam trace elements besi dan tembaga didapatkan sebesar 8.75 mg/kg dan 2,10 mg/kg. Hasil pengujian mikrobiologi dengan metode angka lempeng total dengan menggunakan bakteri Bacillus subtilis dan Eschericia coli didapatakan sebesar 8,6 x 106 koloni/mL dan 2,81 x 107 koloni/mL. Berdasarkan hasil pengamatan morfologi bakteri dengan membandingkannya dengan bakto agar standar komersial secara garis besar tidak jauh berbeda. Sehingga berdasarkan hasil pengujian proksimat dan mikrobiologi, bakto agar penelitian ini dapat digunakan sebagai media pertumbuhan bakteri.Kata kunci: Gelidium sp., Agar, media dan bakteri

scholarly journals Dual bioremediation of phenol and Cr(VI) by mixed microbial cultures in the presence of molasses

2017 ◽  
Vol 75 (12) ◽  
pp. 2883-2890 ◽  
Author(s):  
Nur Koçberber Kiliç ◽  
Gönül Dönmez
Keyword(s):  
Culture Media ◽  
Mineral Salt Medium ◽  
Phenol Removal ◽  
Microbial Culture ◽  
Mixed Microbial Culture ◽  
Ph Values ◽  
Optimum Ph ◽  
Petroleum Contaminated Soil ◽  
Mixed Microbial Cultures ◽  
Positive Effect

Simultaneous phenol and Cr(VI) bioremoval by two different mixed cultures, from petroleum-contaminated soil (PS) and boron-contaminated wastewater (BW), was investigated in regard to different culture media, pH levels (6–8), initial phenol (25–100 mg/L) and Cr(VI) (15–50 mg/L) concentrations. The optimum medium was found to be mineral salt medium tested, which contained 1% (v/v) molasses (MSM). Optimum pH values were 6 for PS and 8 for BW. All of the phenol present in the samples was mineralized regardless of its concentrations tested, Cr(VI) bioremoval was enhanced by the increase in phenol concentrations, and molasses also exerted a positive effect on Cr(VI) removal, and the yields reached 100% for both pollutants, even at 13.1 mg/L Cr(VI) and 91.1 mg/L phenol concentration in PS samples. In MSM containing PS samples approximate efficiency was 100% for phenol removal; but Cr(VI) removal ratios were 64.9% and 41.7% at 25.8 mg/L and 41.3 mg/L concentrations, respectively. Finally it can be concluded that molasses stimulated Cr(VI) bioremoval at elevated phenolic conditions in the mixed microbial culture, and molasses might be of use for the bioremediation of phenol and Cr(VI) polluted wastewaters.

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