Selective Synchronization of Tetrahymena pyriformis Cell Populations and Cell Growth Kinetics during the Cell Cycle

1998 ◽  
Vol 14 (3) ◽  
pp. 450-456 ◽  
Author(s):  
J.-J. Liou ◽  
A.G. Fredrickson ◽  
F. Srienc
Keyword(s):  
Cell Cycle ◽  
Cell Growth ◽  
Growth Kinetics ◽  
Tetrahymena Pyriformis ◽  
Cell Populations ◽  
Cell Growth Kinetics

scholarly journals Effects of Fas-Ligand Overexpression on Alveolar Type II Cell Growth Kinetics in Perinatal Murine Lungs

2010 ◽  
Vol 68 (1) ◽  
pp. 57-62 ◽  
Author(s):  
Monique E de Paepe ◽  
Sheila A Haley ◽  
Zacharie Lacourse ◽  
Quanfu Mao
Keyword(s):  
Cell Growth ◽  
Growth Kinetics ◽  
Fas Ligand ◽  
Alveolar Type ◽  
Type Ii ◽  
Alveolar Type Ii Cell ◽  
Type Ii Cell ◽  
Cell Growth Kinetics

scholarly journals Analysis of BHK cell growth kinetics after microinjection of catalytic subunit of cyclic AMP-dependent protein kinase.

1984 ◽  
Vol 4 (6) ◽  
pp. 1079-1085 ◽  
Author(s):  
J K McClung ◽  
R F Kletzien
Keyword(s):  
Protein Kinase ◽  
Cell Growth ◽  
Cyclic Amp ◽  
Growth Kinetics ◽  
S Phase ◽  
G1 Phase ◽  
Dependent Protein Kinase ◽  
Bhk Cells ◽  
Dependent Protein ◽  
Cell Growth Kinetics

The effect of catalytic subunit (C) of cyclic AMP-dependent protein kinase on cell growth kinetics of BHK cells was assessed by microinjection with chicken erythrocyte ghosts as vehicles for introduction of the protein into the cytosol of large populations of cells. The advantage in using chicken erythrocytes for microinjection is that the inactive erythrocyte nuclei serve as a probe for identifying and analyzing microinjection events. By utilizing this procedure, BHK cells were microinjected with an amount of C that was 5- to 10-fold greater than their endogenous levels. Growth kinetics were analyzed by [3H]thymidine incorporation and autoradiography. Cells were stained after autoradiography to more clearly reveal the chicken nuclei, and at each time point, cells were categorized into four groups: (i) not microinjected, not in S phase, (ii) not microinjected, in S phase, (iii) microinjected, not in S phase, (iv) microinjected, in S phase. Those cells not microinjected served as internal controls. Two experimental protocols were used to test the notion that C is involved in blocking cell progression through G1 phase of the cell cycle. First, cells were arrested in G0 phase by serum deprivation, microinjected with C or control proteins, and stimulated to proceed to S phase by the addition of serum or purified growth factors. Second, cells were collected in mitosis, microinjected with C or control proteins, and stimulated to proceed to S phase by the addition of serum. The results of these studies indicate that a 5- to 10-fold increase in the intracellular concentration of C is not a sufficient signal to arrest cell growth in G1 phase. Thus, growth-inhibitory effects of cyclic AMP on BHK cells are unlikely to be the result of activation of cyclic AMP-dependent protein kinase.

scholarly journals Effect of low dose rate radiation on cell growth kinetics

1979 ◽  
Vol 28 (1) ◽  
pp. 81-91 ◽  
Author(s):  
E.C. Gregg ◽  
T.M. Yau ◽  
S.C. Kim
Keyword(s):  
Cell Growth ◽  
Dose Rate ◽  
Growth Kinetics ◽  
Low Dose ◽  
Low Dose Rate ◽  
Cell Growth Kinetics

scholarly journals Modulation of vascular cell growth kinetics by local cytokine delivery from fibrin glue suspensions

1999 ◽  
Vol 29 (5) ◽  
pp. 852-862 ◽  
Author(s):  
Paula K. Shireman ◽  
Brian Hampton ◽  
Wilson H. Burgess ◽  
Howard P. Greisler
Keyword(s):  
Cell Growth ◽  
Fibrin Glue ◽  
Growth Kinetics ◽  
Vascular Cell ◽  
Local Cytokine ◽  
Cell Growth Kinetics

Mild hyperthermia as a potential mechanism to locally enhance cell growth kinetics

2009 ◽  
Vol 17 (9) ◽  
pp. 719-723 ◽  
Author(s):  
Joseph J. Morrissey ◽  
Ryuji Higashikubo ◽  
Prabhat C. Goswami ◽  
Philip Dixon
Keyword(s):  
Cell Growth ◽  
Growth Kinetics ◽  
Potential Mechanism ◽  
Mild Hyperthermia ◽  
Enhance Cell Growth ◽  
Cell Growth Kinetics

scholarly journals Evaluation of an Ovine Testis Cell Line (OA3.Ts) for Propagation of Capripoxvirus Isolates and Development of an Immunostaining Technique for Viral Plaque Visualization

2007 ◽  
Vol 19 (5) ◽  
pp. 486-491 ◽  
Author(s):  
Shawn Babiuk ◽  
Geoff Parkyn ◽  
John Copps ◽  
June E. Larence ◽  
Marta I. Sabara ◽  
...  
Keyword(s):  
Cell Growth ◽  
Cell Line ◽  
Growth Kinetics ◽  
Cytopathic Effect ◽  
Specific Antiserum ◽  
High Passage ◽  
Viral Plaque ◽  
Cell Growth Kinetics ◽  
Viral Titers

An ovine testis cell line (OA3.Ts) was evaluated and compared with primary lamb kidney (LK) cells for its utility in capripoxvirus propagation and titration. A comparison of OA3.Ts cell growth kinetics and morphology at low (<33) and high (34−36) passage levels indicated a difference in both characteristics. However, viral titers determined in low and high passage OA3.Ts cells were comparable with those obtained using LK cells. Capripoxvirus infection of OA3.Ts and LK cells resulted in a similar cytopathic effect, which allowed for the detection of discrete viral plaques following immunostaining with capripoxvirus-specific antiserum.

Sign in / Sign up

Export Citation Format

Share Document