Higher Ploidy in Saccharomyces cerevisiae Supports Enhanced Hepatitis B Virus S Cloned Gene Expression at the Pilot Scale

1996 ◽  
Vol 12 (1) ◽  
pp. 145-148 ◽  
Author(s):  
J. Fu ◽  
C.B. Parker ◽  
P. Burke ◽  
L.D. Schultz ◽  
D.L. Montgomery ◽  
...  
Keyword(s):  
Gene Expression ◽  
Saccharomyces Cerevisiae ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Pilot Scale ◽  
B Virus ◽  
Cloned Gene

Induction of hepatitis B virus gene expression at low temperature

2000 ◽  
Vol 1490 (1-2) ◽  
pp. 63-73 ◽  
Author(s):  
Marshall J. Kosovsky ◽  
Vladimir I. Khaoustov ◽  
Mary Rushton ◽  
Boris Yoffe
Keyword(s):  
Gene Expression ◽  
Hepatitis B Virus ◽  
Low Temperature ◽  
Hepatitis B ◽  
Virus Gene ◽  
Virus Gene Expression ◽  
B Virus

scholarly journals Development of PCR-ELISA for the detection of hepatitis B virus x gene expression and clinical application

2005 ◽  
Vol 19 (4) ◽  
pp. 139-145 ◽  
Author(s):  
Jong-Wan Kim ◽  
Jung-Hyun Shim ◽  
Joo-Won Park ◽  
Won-Cheol Jang ◽  
H.K. Chang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Clinical Application ◽  
B Virus ◽  
X Gene

scholarly journals Altered Proteolysis and Global Gene Expression in Hepatitis B Virus X Transgenic Mouse Liver

2006 ◽  
Vol 80 (3) ◽  
pp. 1405-1413 ◽  
Author(s):  
Zongyi Hu ◽  
Zhensheng Zhang ◽  
Jin Woo Kim ◽  
Ying Huang ◽  
T. Jake Liang
Keyword(s):  
Gene Expression ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Transgenic Mouse ◽  
Mouse Liver ◽  
Global Gene Expression ◽  
Pleiotropic Effect ◽  
B Virus ◽  
Number Of Genes

ABSTRACT Hepatitis B virus X (HBX) is essential for the productive infection of hepatitis B virus (HBV) in vivo and has a pleiotropic effect on host cells. We have previously demonstrated that the proteasome complex is a cellular target of HBX, that HBX alters the proteolytic activity of proteasome in vitro, and that inhibition of proteasome leads to enhanced viral replication, suggesting that HBX and proteasome interaction plays a crucial role in the life cycle and pathogenesis of HBV. In the present study, we tested the effect of HBX on the proteasome activities in vivo in a transgenic mouse model in which HBX expression is developmentally regulated by the mouse major urinary promoter in the liver. In addition, microarray analysis was performed to examine the effect of HBX expression on the global gene expression profile of the liver. The results showed that the peptidase activities of the proteasome were reduced in the HBX transgenic mouse liver, whereas the activity of another cellular protease was elevated, suggesting a compensatory mechanism in protein degradation. In the microarray analysis, diverse genes were altered in the HBX mouse livers and the number of genes with significant changes increased progressively with age. Functional clustering showed that a number of genes involved in transcription and cell growth were significantly affected in the HBX mice, possibly accounting for the observed pleiotropic effect of HBX. In particular, insulin-like growth factor-binding protein 1 was down-regulated in the HBX mouse liver. The down-regulation was similarly observed during acute woodchuck hepatitis virus infection. Other changes including up-regulation of proteolysis-related genes may also contribute to the profound alterations of liver functions in HBV infection.

scholarly journals Engineered External Guide Sequences Are Highly Effective in Inhibiting Gene Expression and Replication of Hepatitis B Virus in Cultured Cells

PLoS ONE ◽  
2013 ◽  
Vol 8 (6) ◽  
pp. e65268 ◽  
Author(s):  
Zhigang Zhang ◽  
Gia-Phong Vu ◽  
Hao Gong ◽  
Chuan Xia ◽  
Yuan-Chuan Chen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Cultured Cells ◽  
B Virus ◽  
Highly Effective
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