Extractive Cultivation of Recombinant Escherichia coli using Aqueous Two-Phase Systems for Production and Separation of Intracellular Heat Shock Proteins

1996 ◽  
Vol 12 (1) ◽  
pp. 51-56 ◽  
Author(s):  
H. Umakoshi ◽  
K. Yano ◽  
R. Kuboi ◽  
I. Komasawa
Keyword(s):  
Escherichia Coli ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Recombinant Escherichia Coli ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Shock Proteins

scholarly journals Characterization of Surface Properties of Heat Shock Proteins for the Separation Using Aqueous Two-Phase Systems.

1994 ◽  
Vol 27 (6) ◽  
pp. 808-814 ◽  
Author(s):  
Koji Yano ◽  
Tetsuhiro Hasegawa ◽  
Ryoichi Kuboi ◽  
Isao Komasawa ◽  
Tetsuaki Tsuchido
Keyword(s):  
Heat Shock ◽  
Heat Shock Proteins ◽  
Surface Properties ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Shock Proteins

Use of aqueous two-phase systems in the purification of human interferon-α1 from recombinant Escherichia coli

1996 ◽  
Vol 19 (6) ◽  
pp. 446-455 ◽  
Author(s):  
Yue Guan ◽  
Terence H. Lilley ◽  
Timothy E. Treffry ◽  
Chang-Ling Zhou ◽  
Peter B. Wilkinson
Keyword(s):  
Escherichia Coli ◽  
Recombinant Escherichia Coli ◽  
Human Interferon ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

scholarly journals Ionic Liquid Aqueous Two-Phase Systems for the Enhanced Paper-Based Detection of Transferrin and Escherichia coli

2018 ◽  
Vol 6 ◽  
Author(s):  
Matthew F. Yee ◽  
Grace N. Emmel ◽  
Eric J. Yang ◽  
Eumene Lee ◽  
Justin H. Paek ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Ionic Liquid ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

scholarly journals Functional Analysis of Genes Comprising the Locus of Heat Resistance in Escherichia coli

2017 ◽  
Vol 83 (20) ◽  
Author(s):  
Ryan Mercer ◽  
Oanh Nguyen ◽  
Qixing Ou ◽  
Lynn McMullen ◽  
Michael G. Gänzle
Keyword(s):  
Escherichia Coli ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Heat Resistance ◽  
Growth Phase ◽  
Genomic Island ◽  
Enteric Bacteria ◽  
Content Type ◽  
E Coli ◽  
Shock Proteins

ABSTRACT The locus of heat resistance (LHR) is a 15- to 19-kb genomic island conferring exceptional heat resistance to organisms in the family Enterobacteriaceae, including pathogenic strains of Salmonella enterica and Escherichia coli. The complement of LHR-comprising genes that is necessary for heat resistance and the stress-induced or growth-phase-induced expression of LHR-comprising genes are unknown. This study determined the contribution of the seven LHR-comprising genes yfdX1 GI, yfdX2, hdeD GI, orf11, trx GI, kefB, and psiE GI by comparing the heat resistances of E. coli strains harboring plasmid-encoded derivatives of the different LHRs in these genes. (Genes carry a subscript “GI” [genomic island] if an ortholog of the same gene is present in genomes of E. coli.) LHR-encoded heat shock proteins sHSP20, ClpKGI, and sHSPGI are not sufficient for the heat resistance phenotype; YfdX1, YfdX2, and HdeD are necessary to complement the LHR heat shock proteins and to impart a high level of resistance. Deletion of trx GI, kefB, and psiE GI from plasmid-encoded copies of the LHR did not significantly affect heat resistance. The effect of the growth phase and the NaCl concentration on expression from the putative LHR promoter p2 was determined by quantitative reverse transcription-PCR and by a plasmid-encoded p2:GFP promoter fusion. The expression levels of exponential- and stationary-phase E. coli cells were not significantly different, but the addition of 1% NaCl significantly increased LHR expression. Remarkably, LHR expression in E. coli was dependent on a chromosomal copy of evgA. In conclusion, this study improved our understanding of the genes required for exceptional heat resistance in E. coli and factors that increase their expression in food. IMPORTANCE The locus of heat resistance (LHR) is a genomic island conferring exceptional heat resistance to several foodborne pathogens. The exceptional level of heat resistance provided by the LHR questions the control of pathogens by current food processing and preparation techniques. The function of LHR-comprising genes and their regulation, however, remain largely unknown. This study defines a core complement of LHR-encoded proteins that are necessary for heat resistance and demonstrates that regulation of the LHR in E. coli requires a chromosomal copy of the gene encoding EvgA. This study provides insight into the function of a transmissible genomic island that allows otherwise heat-sensitive enteric bacteria, including pathogens, to lead a thermoduric lifestyle and thus contributes to the detection and control of heat-resistant enteric bacteria in food.

Partitioning behavior of recombinant lipase in Escherichia coli by ionic liquid-based aqueous two-phase systems

RSC Advances ◽  
2016 ◽  
Vol 6 (86) ◽  
pp. 82571-82580 ◽  
Author(s):  
Muhammad Hakimi Hadzir ◽  
Sahar Abbasiliasi ◽  
Arbakariya B. Ariff ◽  
Siti Baidurah Yusoff ◽  
Hui Suan Ng ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Ionic Liquid ◽  
Ionic Liquids ◽  
Fermentation Broth ◽  
Two Phase ◽  
E Coli ◽  
Recombinant Lipase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Partitioning Behavior

Evaluations of ILATPSs were performed with a variety of ionic liquids and salts as phase components to figure out their competencies in the recovery of lipase from a fermentation broth of E. coli using banana waste as a substrate.

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