scholarly journals Production and Characterization of ZFP36L1 Antiserum against Recombinant Protein from Escherichia coli

2008 ◽  
Vol 24 (2) ◽  
pp. 326-333 ◽  
Author(s):  
H. Cao ◽  
R. Lin ◽  
S. Ghosh ◽  
R.A. Anderson ◽  
J.F. Urban
Keyword(s):  
Escherichia Coli ◽  
Recombinant Protein

Expression and characterization of the 42 kDa chitinase of the biocontrol fungusMetarhizium anisopliaeinEscherichia coli

2003 ◽  
Vol 49 (11) ◽  
pp. 723-726 ◽  
Author(s):  
César Milton Baratto ◽  
Marcia Vanusa da Silva ◽  
Lucélia Santi ◽  
Luciane Passaglia ◽  
Irene Silveira Schrank ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Recombinant Protein ◽  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Expression Vector ◽  
Hydrolytic Enzymes ◽  
Active Protein ◽  
Chitinase Genes

Albeit Metarhizium anisopliae is the best-characterized entomopathogenic fungus, the role of some hydrolytic enzymes during host cuticle penetration has not yet been established. Three chitinase genes (chit1, chi2, chi3) from Metarhizium have already been isolated. To characterize the chitinase coded by the chit1 gene, we expressed the active protein (CHIT42) in Escherichia coli using a T7-based promoter expression vector. The recombinant protein, CHIT42, is active against glycol chitin and synthetic N-acetylglucosamine (GlcNAc) dimer and tetramer substrates. These activities suggest that the recombinant CHIT42 acts as an endochitinase.Key words: Metarhizium anisopliae, chitinases, chit genes, recombinant protein, enthomopathogenic fungi.

scholarly journals Cloning and expression of Saccharomyces cerevisiae copper-metallothionein gene in Escherichia coli and characterization of the recombinant protein

1993 ◽  
Vol 212 (2) ◽  
pp. 521-528 ◽  
Author(s):  
Zehra SAYERS ◽  
Patricia BROUILLON ◽  
Constantin E. VORGIAS ◽  
Hans F. NOLTING ◽  
Christoph HERMES ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Saccharomyces Cerevisiae ◽  
Recombinant Protein ◽  
Cloning And Expression ◽  
Metallothionein Gene

Expression of a Brassic napus glutamate 1-semialdehyde aminotransferase in Escherichia coli and characterization of the recombinant protein

2003 ◽  
Vol 29 (2) ◽  
pp. 193-201 ◽  
Author(s):  
Edward W.T Tsang ◽  
Zhiyuan Hu ◽  
Qing Chang ◽  
D.Ian McGregor ◽  
Wilfred A Keller
Keyword(s):  
Escherichia Coli ◽  
Recombinant Protein

Production and characterization of ZFP36L1 antiserum against recombinant protein from Escherichia coli

2008 ◽  
Vol 22 (S1) ◽  
Author(s):  
Heping Cao ◽  
Rui Lin ◽  
Sanjukta Ghosh ◽  
Richard A. Anderson ◽  
Joseph F. Urban
Keyword(s):  
Escherichia Coli ◽  
Recombinant Protein
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