Metabolic Engineering and Characterization of phaC1 and phaC2 Genes from Pseudomonas putida KCTC1639 for Overproduction of Medium-Chain-Length Polyhydroxyalkanoate

2006 ◽  
Vol 22 (6) ◽  
pp. 1541-1546 ◽  
Author(s):  
T.-K. Kim ◽  
Y.-M. Jung ◽  
M.T. Vo ◽  
S. Shioya ◽  
Y.-H. Lee
Keyword(s):  
Metabolic Engineering ◽  
Pseudomonas Putida ◽  
Chain Length ◽  
Medium Chain ◽  
Medium Chain Length

scholarly journals Overexpression and characterization of medium-chain-length polyhydroxyalkanoate granule bound polymerases from Pseudomonas putida GPo1

2009 ◽  
Vol 8 (1) ◽  
pp. 60 ◽  
Author(s):  
Qun Ren ◽  
Guy de Roo ◽  
Bernard Witholt ◽  
Manfred Zinn ◽  
Linda Thöny-Meyer
Keyword(s):  
Pseudomonas Putida ◽  
Chain Length ◽  
Medium Chain ◽  
Medium Chain Length

Kinetics of medium-chain-length polyhydroxyalkanoate production by a novel isolate of Pseudomonas putida LS46

2012 ◽  
Vol 58 (8) ◽  
pp. 982-989 ◽  
Author(s):  
Parveen K. Sharma ◽  
Jilagamazhi Fu ◽  
Nazim Cicek ◽  
Richard Sparling ◽  
David B. Levin
Keyword(s):  
Pseudomonas Putida ◽  
Chain Length ◽  
Saturated Fatty Acids ◽  
Dry Mass ◽  
Nucleotide Sequence Analysis ◽  
Glucose Medium ◽  
Medium Chain ◽  
Medium Chain Length ◽  
Pha Production ◽  
Nitrogen Excess

Six bacteria that synthesize medium-chain-length polyhydroxyalkanoates (mcl-PHAs) were isolated from sewage sludge and hog barn wash and identified as strains of Pseudomonas and Comamonas by 16S rDNA gene sequencing. One isolate, Pseudomonas putida LS46, showed good PHA production (22% of cell dry mass) in glucose medium, and it was selected for further studies. While it is closely related to other P. putida strains (F1, KT2440, BIRD-1, GB-1, S16, and W619), P. putida LS46 was genetically distinct from these other strains on the basis of nucleotide sequence analysis of the cpn60 gene hypervariable region. PHA production was detected as early as 12 h in both nitrogen-limited and nitrogen-excess conditions. The increase in PHA production after 48 h was higher in nitrogen-limited cultures than in nitrogen-excess cultures. Pseudomonas putida LS46 produced mcl-PHAs when cultured with glucose, glycerol, or C6–C14 saturated fatty acids as carbon sources, and mcl-PHAs accounted for 56% of the cell dry mass when cells were batch cultured in medium containing 20 mmol/L octanoate. Although 3-hydroxydecanoate was the major mcl-PHA monomer (58.1–68.8 mol%) in P. putida LS46 cultured in glucose medium, 3-hydroxyoctanoate was the major monomer produced in octanoate medium (88 mol%).

scholarly journals Draft Genome Sequence of Medium-Chain-Length Polyhydroxyalkanoate-Producing Pseudomonas putida Strain LS46

2013 ◽  
Vol 1 (2) ◽  
Author(s):  
P. K. Sharma ◽  
J. Fu ◽  
X. Zhang ◽  
B. W. Fristensky ◽  
K. Davenport ◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Chain Length ◽  
Genome Sequence ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Medium Chain ◽  
Medium Chain Length

scholarly journals Quantitative ‘Omics Analyses of Medium Chain Length Polyhydroxyalkanaote Metabolism in Pseudomonas putida LS46 Cultured with Waste Glycerol and Waste Fatty Acids

PLoS ONE ◽  
2015 ◽  
Vol 10 (11) ◽  
pp. e0142322 ◽  
Author(s):  
Jilagamazhi Fu ◽  
Parveen Sharma ◽  
Vic Spicer ◽  
Oleg V. Krokhin ◽  
Xiangli Zhang ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Pseudomonas Putida ◽  
Chain Length ◽  
Medium Chain ◽  
Waste Glycerol ◽  
Medium Chain Length

Metabolic Engineering and Characterization ofphaC1andphaC2Genes fromPseudomonas putidaKCTC1639 for Overproduction of Medium-Chain-Length Polyhydroxyalkanoate

2006 ◽  
Vol 22 (6) ◽  
pp. 1541-1546 ◽  
Author(s):  
Tae-Kwon Kim ◽  
Young-Mi Jung ◽  
Minh Tri Vo ◽  
Suteaki Shioya ◽  
Yong-Hyun Lee
Keyword(s):  
Metabolic Engineering ◽  
Chain Length ◽  
Medium Chain ◽  
Medium Chain Length

scholarly journals Molecular Basis of Medium-Chain Length-PHA Metabolism of Pseudomonas putida

2020 ◽  
pp. 89-114
Author(s):  
Maria Tsampika Manoli ◽  
Natalia Tarazona ◽  
Aranzazu Mato ◽  
Beatriz Maestro ◽  
Jesús M. Sanz ◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Chain Length ◽  
Molecular Basis ◽  
Medium Chain ◽  
Medium Chain Length
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