Alkaline Protease Production by an Isolated Bacillus circulans under Solid-State Fermentation Using Agroindustrial Waste: Process Parameters Optimization

2005 ◽  
Vol 21 (5) ◽  
pp. 1380-1388 ◽  
Author(s):  
R.S. Prakasham ◽  
Ch.S. Rao ◽  
R.S. Rao ◽  
P.N. Sarma
Keyword(s):  
Solid State ◽  
Process Parameters ◽  
Solid State Fermentation ◽  
Alkaline Protease ◽  
Parameters Optimization ◽  
Bacillus Circulans ◽  
Protease Production ◽  
Process Parameters Optimization ◽  
Agroindustrial Waste

Alkaline Protease Production from Brevibacterium luteolum (MTCC 5982) Under Solid-State Fermentation and Its Application for Sulfide-Free Unhairing of Cowhides

2016 ◽  
Vol 182 (2) ◽  
pp. 511-528 ◽  
Author(s):  
R. Renganath Rao ◽  
M. Vimudha ◽  
N. R. Kamini ◽  
M. K. Gowthaman ◽  
B. Chandrasekran ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Alkaline Protease ◽  
Protease Production

Optimization of Nutritional parameters by One-Factor-At-A-Time method for the Biosynthesis of Alkaline Protease from isolated strain Alternaria alternata TUSGF1

2021 ◽  
Vol 10 ◽  
Author(s):  
Tapasi Polley ◽  
Uma Ghosh
Keyword(s):  
Particle Size ◽  
Solid State ◽  
Solid State Fermentation ◽  
Alternaria Alternata ◽  
Alkaline Protease ◽  
Carbon Sources ◽  
Skim Milk ◽  
Protease Production ◽  
Agricultural Residue ◽  
Nutritional Parameters

Background: Alkaline protease essential enzymes that have several applications in our industry. Objective: The aim was optimization of nutritional parameters by one-factor-at-a-time (OFAT) method in solid-state fermentation. Method: Production of protease employing our laboratory new isolate, Alternaria alternata TUSG1 (strain accession number- MF401426) under solid-state fermentation was optimized. The nutritional factors was investigated and only one agricultural residue (cauliflower leaves) with different particle size was checked. Results: Highest enzyme production was obtained with medium particle size of cauliflower leaves (610 U/gds) followed by coarse waste (603U/gds) and fine waste (596 U/gds) using 106 spores/ml as inoculum at 30° C for 7 days. The organism utilized carbon sources 0.5 % (w/w) dextrose, fructose, maltose, sucrose, lactose and starch. Among them maltose was found to be the best carbon source. A variety of inorganic and organic media components were investigated for nitrogen sources 0.3 % (w/w) and skim milk turned out to the best. Conclusion: The maximum enzyme activity was obtained with 1% maltose, 0.5% skim milk and 0.05% MgSO4. With optimized media 1.53 fold increase in the protease production at agricultural residue cauliflower leaves was obtained.

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