Role of Arginine in Protein Refolding, Solubilization, and Purification

2004 ◽  
Vol 20 (5) ◽  
pp. 1301-1308 ◽  
Author(s):  
K. Tsumoto ◽  
M. Umetsu ◽  
I. Kumagai ◽  
D. Ejima ◽  
J.S. Philo ◽  
...  
Keyword(s):  
Protein Refolding

scholarly journals Role of ionic liquids in protein refolding: native/fibrillar versus treated lysozyme

RSC Advances ◽  
2012 ◽  
Vol 2 (32) ◽  
pp. 12329 ◽  
Author(s):  
Sara Mangialardo ◽  
Lorenzo Gontrani ◽  
Francesca Leonelli ◽  
Ruggero Caminiti ◽  
Paolo Postorino
Keyword(s):  
Ionic Liquids ◽  
Protein Refolding

Development of Novel Rhodacyanine-Based Heat Shock Protein 70 Inhibitors

2021 ◽  
Vol 28 ◽  
Author(s):  
Chih-Shiang Chang ◽  
Vathan Kumar ◽  
Der-Yen Lee ◽  
Yeh Chen ◽  
Yu-Chieh Wu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Protein Refolding ◽  
Hsp70 Expression ◽  
Antitumor Activities ◽  
Hsp90 Expression ◽  
Client Proteins ◽  
Hsp70 Inhibitors ◽  
Established Role ◽  
New Strategies

Background: A growing body of evidence suggests that Hsp70, which is overexpressed in human breast tumors, plays a role in tumorigenesis and tumor progression in breast cancer as well as in its aggressive phenotypes. Hsp70 constitutes a potential therapeutic target in the treatment of this disease. Method: We developed a new series of rhodacyanine-based Hsp70 inhibitors, represented by compounds 1 and 6, in which the cationic pyridin-1-ium or thiazol-3-ium ring of existing Hsp70 inhibitors (e.g., JG-40 and JG-98) was replaced by a corresponding benzo-fused N-heterocycle. Results: Several lines of evidence suggest that these benzo-fused derivatives may exert their antitumor activities, in part, by targeting Hsp70. These putative inhibitors displayed differential antiproliferative efficacy against breast cancer cells (IC50 as low as 0.25 µM) versus nontumorigenic MCF-10A breast epithelial cells (IC50 ≥ 5 µM). This was correlated with the corresponding Hsp70 expression levels. Using a protein refolding assay, we confirmed that these agents effectively inhibited the chaperone activity of Hsp70. Moreover, these inhibitors effectively suppressed the expression of well-known oncogenic client proteins of Hsp70’s, including FoxM1, HuR, and Akt, which paralleled their antiproliferative efficacy. Supporting the established role of Hsp70 in regulating protein refolding, these derivatives induced autophagy, as manifested by the conversion of LC3B-I to LC3B-II. Notably, these putative Hsp70 inhibitors did not cause a compensatory elevation in Hsp90 expression, contrasting with the previously reported effects of Hsp90 inhibitors on Hsp70 upregulation. Conclusion: Together with the finding that compounds 1 and 6 showed improved microsomal stability, these results suggest the translational potential of these putative Hsp70 inhibitors to foster new strategies for cancer therapy. However, whether these benzo-fused rhodacyanines act on kinases or other targets remains unclear, which is currently under investigation.

Elucidating the Role of Thermal Flexibility of Hydrogels in Protein Refolding

2020 ◽  
Vol 3 (7) ◽  
pp. 4253-4262 ◽  
Author(s):  
Anayet Kabir ◽  
Marya Ahmed
Keyword(s):  
Protein Refolding

Role of Stimuli-Sensitive Polymers in Protein Refolding:  α-Amylase and CcdB (Controller of Cell Division or Death B) as Model Proteins†

Langmuir ◽  
2007 ◽  
Vol 23 (1) ◽  
pp. 70-75 ◽  
Author(s):  
Kalyani Mondal ◽  
Smita Raghava ◽  
Bipasha Barua ◽  
Raghavan Varadarajan ◽  
Munishwar N. Gupta
Keyword(s):  
Cell Division ◽  
Protein Refolding ◽  
Stimuli Sensitive

Role of dactinomycin in the improved survival of children with Wilms' tumor

JAMA ◽  
1966 ◽  
Vol 195 (12) ◽  
pp. 1005-1009 ◽  
Author(s):  
D. J. Fernbach
Keyword(s):  
Wilms Tumor

Role of the allergist in diagnosis and management of patients with uveitis

JAMA ◽  
1966 ◽  
Vol 195 (3) ◽  
pp. 167-172 ◽  
Author(s):  
T. E. Van Metre
Keyword(s):  
Diagnosis And Management

The power of norms to sway fused group members

2018 ◽  
Vol 41 ◽  
Author(s):  
Winnifred R. Louis ◽  
Craig McGarty ◽  
Emma F. Thomas ◽  
Catherine E. Amiot ◽  
Fathali M. Moghaddam
Keyword(s):  
Evolutionary Anthropology ◽  
Normative Systems ◽  
Violent Extremism ◽  
Identity Fusion ◽  
Group Members

AbstractWhitehouse adapts insights from evolutionary anthropology to interpret extreme self-sacrifice through the concept of identity fusion. The model neglects the role of normative systems in shaping behaviors, especially in relation to violent extremism. In peaceful groups, increasing fusion will actually decrease extremism. Groups collectively appraise threats and opportunities, actively debate action options, and rarely choose violence toward self or others.

Elaborating the role of reflection and individual differences in the study of folk-economic beliefs

2018 ◽  
Vol 41 ◽  
Author(s):  
Kevin Arceneaux
Keyword(s):  
Decision Making ◽  
Individual Differences ◽  
Cognitive Processes ◽  
Evolutionary History ◽  
Behavioral Responses ◽  
History Of ◽  
Economic Beliefs

AbstractIntuitions guide decision-making, and looking to the evolutionary history of humans illuminates why some behavioral responses are more intuitive than others. Yet a place remains for cognitive processes to second-guess intuitive responses – that is, to be reflective – and individual differences abound in automatic, intuitive processing as well.

The role of affect in feelings of obligation

2020 ◽  
Vol 43 ◽  
Author(s):  
Stefen Beeler-Duden ◽  
Meltem Yucel ◽  
Amrisha Vaish
Keyword(s):  
Positive Emotions ◽  
The Creation ◽  
Sense Of Obligation

Abstract Tomasello offers a compelling account of the emergence of humans’ sense of obligation. We suggest that more needs to be said about the role of affect in the creation of obligations. We also argue that positive emotions such as gratitude evolved to encourage individuals to fulfill cooperative obligations without the negative quality that Tomasello proposes is inherent in obligations.

Refining our understanding of the “elephant in the room”

2020 ◽  
Vol 43 ◽  
Author(s):  
Andrew Whiten
Keyword(s):  
Social Cognition ◽  
Cultural Evolution ◽  
Cognitive Abilities ◽  
Critical Factors ◽  
Technological Culture

Abstract The authors do the field of cultural evolution a service by exploring the role of non-social cognition in human cumulative technological culture, truly neglected in comparison with socio-cognitive abilities frequently assumed to be the primary drivers. Some specifics of their delineation of the critical factors are problematic, however. I highlight recent chimpanzee–human comparative findings that should help refine such analyses.

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