Dilute Acid Pretreatment, Enzymatic Saccharification, and Fermentation of Rice Hulls to Ethanol

2008 ◽  
Vol 21 (3) ◽  
pp. 816-822 ◽  
Author(s):  
Badal C. Saha ◽  
Loren B. Iten ◽  
Michael A. Cotta ◽  
Y. Victor Wu
Keyword(s):  
Enzymatic Saccharification ◽  
Dilute Acid Pretreatment ◽  
Dilute Acid ◽  
Acid Pretreatment ◽  
Rice Hulls

Dilute acid pretreatment of starch-containing rice hulls for ethanol production

Holzforschung ◽  
2011 ◽  
Vol 65 (4) ◽  
Author(s):  
Yoney López ◽  
Beatriz Gullón ◽  
Jürgen Puls ◽  
Juan C. Parajó ◽  
Carlos Martín
Keyword(s):  
Ethanol Production ◽  
Sulfuric Acid Concentration ◽  
Enzymatic Saccharification ◽  
Raw Material ◽  
Dilute Acid Pretreatment ◽  
Small Scale ◽  
Dilute Acid ◽  
Acid Pretreatment ◽  
Rice Hulls ◽  
Pretreatment Conditions

Abstract Rice hulls are potential low-cost feedstocks for fuel ethanol production in many countries. Most of the relevant publications are devoted to homogenous (high quality) hulls generated in industrial mills. On the other hand, small-scale mills give rise to hulls containing grain fragments and bran. In the present work, the dilute-acid pretreatment of such heterogeneous rice hulls was investigated based on a central composite rotatable experimental design. The variables were: temperature (140–210°C), biomass load (5–20%), and sulfuric acid concentration (0.5–1.5% based on reaction mixture). A total of 16 experimental runs were carried out, including a 23-plan, two replicates at the central point and six star points. Low temperatures were found to be favorable for the hydrolysis of xylan and of the easily hydrolyzable glucan fraction. High glucose formation (up to 15.3% of the raw material dry weight), attributable to starch hydrolysis, was detected in the hydrolysates obtained under the least severe pretreatment conditions. Several models were developed for predicting the effect of the operational conditions on the yield of pretreated solids, xylan and glucan conversion upon pretreatment, and on enzymatic convertibility of cellulose. The pretreatment temperature exerted the most significant effect on the conversion of the polysaccharides. Optimum results were predicted for the conversion of easily-hydrolyzable glucan in the material pretreated at 140.7°C, and for the enzymatic saccharification of cellulose in the material pretreated at 169°C. These results are interpreted that a two-step acid hydrolysis may be the best pretreatment strategy for heterogeneous rice hulls produced in small mills.

scholarly journals Enhanced Enzymatic Saccharification of Mixed Sawdust Wastes: Comparison of SPORL, Dilute Acid, Formic Acid, and Ethanol Organosolv Pretreatments

2021 ◽  
Vol 65 (3) ◽  
Author(s):  
Yuan Liang ◽  
Yanbo Yin ◽  
Haifeng Zhou ◽  
Yuanyu Tian
Keyword(s):  
Formic Acid ◽  
Enzymatic Saccharification ◽  
Fiber Surface ◽  
Dilute Acid Pretreatment ◽  
Dilute Acid ◽  
Acid Pretreatment ◽  
Glucose Yield ◽  
Hemicellulose Removal ◽  
Ethanol Pretreatment ◽  
Hydrolysis Of

Abstract. Utilization of the huge quantity of sawdust wastes is urgent. In this study, SPORL, dilute acid pretreatment (DA), formic acid pretreatment (FA), ethanol pretreatment (EtOH/H2O), and sulfuric acid catalyzed ethanol pretreatment (EtOH/H2O/H2SO4), on improving enzymatic hydrolysis of mixed sawdust wastes were comprehensively compared. EtOH/H2O/H2SO4 was the most effective pretreatment in lignin removal from sawdust fiber cell wall, while FA was much more effective in hemicellulose removal. After the pretreatments, the crystallinity of cellulose increased because of the removal of amorphous hemicellulose and lignin. Moreover, the fiber surface became coarse and porous, especially after EtOH/H2O/H2SO4, the structure was destroyed into fragments, which enhanced the cellulase accessibility of cellulose. Therefore, the glucose yield of EtOH/H2O/H2SO4 pretreated substrate was highest among these five pretreatments, achieved at 91.4% with a cellulase loading of only 10 FPU/g glucan.   Resumen. Es urgente aprovechar la gran cantidad de residuos de aserrín. En este estudio, SPORL, pretratamiento con ácido diluido (DA), pretratamiento con ácido fórmico (FA), pretratamiento con etanol (EtOH/H2O) y pretratamiento con etanol catalizado con ácido sulfúrico (EtOH/H2O/H2SO4), sobre la mejora de la hidrólisis enzimática de residuos de aserrín mezclado fueron comparados de manera integral. EtOH/H2O/H2SO4 fue el pretratamiento más eficaz para eliminar la lignina de la pared celular de la fibra de aserrín, mientras que el FA fue mucho más eficaz para eliminar la hemicelulosa. Después de los pretratamientos, la cristalinidad de la celulosa aumentó debido a la eliminación de hemicelulosa amorfa y lignina. Además, la superficie de la fibra se volvió gruesa y porosa, especialmente después de EtOH/H2O/H2SO4, la estructura se destruyó en fragmentos, lo que mejoró la accesibilidad de celulasa de la celulosa. Por lo tanto, el rendimiento de glucosa del sustrato pretratado con EtOH/H2O/H2SO4 fue el más alto entre estos cinco pretratamientos, alcanzado al 91,4% con una carga de celulasa de solo 10 FPU / g de glucano.

Dilute acid pretreatment, enzymatic saccharification and fermentation of wheat straw to ethanol

2005 ◽  
Vol 40 (12) ◽  
pp. 3693-3700 ◽  
Author(s):  
Badal C. Saha ◽  
Loren B. Iten ◽  
Michael A. Cotta ◽  
Y. Victor Wu
Keyword(s):  
Wheat Straw ◽  
Enzymatic Saccharification ◽  
Dilute Acid Pretreatment ◽  
Dilute Acid ◽  
Acid Pretreatment

Reducing acid in dilute acid pretreatment and the impact on enzymatic saccharification

2011 ◽  
Vol 39 (5) ◽  
pp. 691-700 ◽  
Author(s):  
Ye Chen ◽  
Mark A. Stevens ◽  
Yongming Zhu ◽  
Jason Holmes ◽  
Geoffrey Moxley ◽  
...  
Keyword(s):  
Enzymatic Saccharification ◽  
Dilute Acid Pretreatment ◽  
Dilute Acid ◽  
Acid Pretreatment ◽  
The Impact

Intensification of bioethanol production by using Tween 80 to enhance dilute acid pretreatment and enzymatic saccharification of corncob

2018 ◽  
Vol 124 ◽  
pp. 166-176 ◽  
Author(s):  
Eduardo K. Kleingesinds ◽  
Álvaro H.M. José ◽  
Larissa P. Brumano ◽  
Talita Silva-Fernandes ◽  
Durval Rodrigues ◽  
...  
Keyword(s):  
Enzymatic Saccharification ◽  
Tween 80 ◽  
Dilute Acid Pretreatment ◽  
Bioethanol Production ◽  
Dilute Acid ◽  
Acid Pretreatment
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