Simultaneous Saccharification and Co-Fermentation of Crystalline Cellulose and Sugar Cane Bagasse Hemicellulose Hydrolysate to Lactate by a Thermotolerant Acidophilic Bacillus sp.

2005 ◽  
Vol 21 (5) ◽  
pp. 1453-1460 ◽  
Author(s):  
M.A. Patel ◽  
M.S. Ou ◽  
L.O. Ingram ◽  
K.T. Shanmugam
Keyword(s):  
Sugar Cane ◽  
Sugar Cane Bagasse ◽  
Crystalline Cellulose ◽  
Bacillus Sp ◽  
Hemicellulose Hydrolysate ◽  
Simultaneous Saccharification

Optimation xilitol production with variation of sugar cane bagasse hemicellulose hydrolysate concentration by Candida tropicalis

2004 ◽  
Vol 2 (1) ◽  
pp. 29-34
Author(s):  
WAHYUNI WAHYUNI ◽  
ARI SUSILOWATI ◽  
RATNA SETYANINGSIH
Keyword(s):  
Sugar Cane ◽  
High Performance ◽  
Liquid Fraction ◽  
High Performance Liquid Chromatographic ◽  
Sugar Cane Bagasse ◽  
Production Medium ◽  
Hemicellulose Hydrolysate ◽  
The Third ◽  
Frame Work ◽  
Liquid Chromatographic

The aims of this research were to study the growth of C. tropicalis, the optimation of xilitol production and the efficiency of xilitol production by varying the concentration of sugar cane bagasse hemicellulose hydrolysate. The frame work of this research was bioconversion xilosa into xilitol by C. tropicalis influenced substrate concentration in production medium. By using different sugar cane bagasse hemicellulose hydrolysate concentration could be known the optimum sugar cane bagasse hemicellulose hydrolysate concentration on xilitol production by introducing C. tropicalis. The methods used in this experiment were as follow: sugar cane bagasse was hydrolyzed by H2SO4 0.035 M in autoclave at 121ï‚°C, 2 atm for 20 minutes. The liquid fraction was concentrated at 50ï‚°C using rotary evaporator. The hydrolysate was neutralized with Ca(OH)2 to a pH of 10 and then removed by centrifugation at 2000 rpm for 20 minutes. The hydrolysate was added with H2SO4 to a pH 6.5 and then removed by centrifugation at 2000 rpm for 20 minutes and sterilized by autoclaving at 121ï‚°C for 15 minutes. Sugar cane bagasse hemicellulose hydrolysate concentration was variated from 10%, 20%, and 30% and used as substrate in production medium. Bioconversion process by C. tropicalis lasted for 4 days. Parameters used in this experiment were biomass of C. tropicalis measured by hemacytometer, xilosa and xilitol concentration in the sugar cane bagasse hemicellulose hydrolysate and production medium was analyzed by High Performance Liquid Chromatographic (HPLC). The results showed that: the growth of C. tropicalis lasted for 4 days of cultivation increased in every variation of sugar cane hemicellulose hydrolysate concentration. The optimum xilitol production founded in production medium contained 20% sugar cane bagasse hemicellulose hydrolysate in the third days cultivation with xilitol production 10.258 g/l, yield 0.22 g/g and biomass of C. tropicalis 2.9 x 108 cell/ml; The optimum efficiency of xilitol production from sugar cane bagasse hemicellulose hydrolysate by C. tropicalis was 24.21% in 20% sugar cane bagasse hemicellulose hydrolysate in the third days cultivation.

scholarly journals Experimental determination of the effects of pretreatment on selected Nigerian lignocellulosic biomass in bioethanol production

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Adeolu A. Awoyale ◽  
David Lokhat
Keyword(s):  
Lignocellulosic Biomass ◽  
Sugar Cane ◽  
Sugar Cane Bagasse ◽  
Crystalline Cellulose ◽  
Cellulose Content ◽  
Bioethanol Production ◽  
Rice Husks ◽  
Pore Characteristics ◽  
Corn Cobs ◽  
Cassava Peels

AbstractIn the present study, five lignocellulosic biomass namely, corn cobs (Zea mays), rice husks (Oryza sativa), cassava peels (Manihot esculenta), sugar cane bagasse (Saccharum officinarum), and white yam peels (Dioscorea rotundata) of two mesh sizes of 300 and 425 microns and a combination of some and all of the biomass were pretreated using combined hydrothermal and acid-based, combined hydrothermal and alkali-based and hydrothermal only processes. The raw and pretreated biomass were also characterized by Fourier transform infrared spectroscopy (FT-IR), Brunauer–Emmett–Teller (BET), X-Ray diffraction (XRD), and Scanning electron microscopy (SEM) to determine the effects of the various pretreatments on the biomass being studied. The cellulose values of the raw biomass range from 25.8 wt% for cassava peels biomass to 40.0 wt% for sugar cane bagasse. The values of the cellulose content increased slightly with the pretreatment, ranging from 33.2 to 43.8 wt%. The results of the analysis indicate that the hydrothermal and alkaline-based pretreatment shows more severity on the different biomass being studied as seen from the pore characteristics results of corn cobs + rice husks biomass, which also shows that the combination of feedstocks can effectively improve the properties of the biomass in the bioethanol production process. The FTIR analysis also showed that the crystalline cellulose present in all the biomass was converted to the amorphous form after the pretreatment processes. The pore characteristics for mixed corn cobs and rice husks biomass have the highest specific surface area and pore volume of 1837 m2/g and 0.5570 cc/g respectively.

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