Enhanced Isoamyl Acetate Production upon Manipulation of the Acetyl-CoA Node in Escherichia coli

2004 ◽  
Vol 20 (3) ◽  
pp. 692-697 ◽  
Author(s):  
R.V. Vadali ◽  
G.N. Bennett ◽  
K.-Y. San
Keyword(s):  
Escherichia Coli ◽  
Isoamyl Acetate ◽  
Acetate Production ◽  
Acetyl Coa

Intensification of isoamyl acetate production: transport properties of silica membranes

2013 ◽  
Vol 51 (10-12) ◽  
pp. 2377-2386 ◽  
Author(s):  
Wilmar Osorio-Viana ◽  
Jesús-David Quintero-Arias ◽  
Izabela Dobrosz-Gómez ◽  
Javier Fontalvo ◽  
Miguel Ángel Gómez-García
Keyword(s):  
Transport Properties ◽  
Isoamyl Acetate ◽  
Acetate Production ◽  
Silica Membranes

scholarly journals Acetate and Formate Stress: Opposite Responses in the Proteome of Escherichia coli

2001 ◽  
Vol 183 (21) ◽  
pp. 6466-6477 ◽  
Author(s):  
Christopher Kirkpatrick ◽  
Lisa M. Maurer ◽  
Nikki E. Oyelakin ◽  
Yuliya N. Yoncheva ◽  
Russell Maurer ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Opposite Effect ◽  
Stress Proteins ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Acid Resistance ◽  
Luria Broth ◽  
Acetyl Coa ◽  
Fermentation Products ◽  
E Coli

ABSTRACT Acetate and formate are major fermentation products ofEscherichia coli. Below pH 7, the balance shifts to lactate; an oversupply of acetate or formate retards growth. E. coli W3110 was grown with aeration in potassium-modified Luria broth buffered at pH 6.7 in the presence or absence of added acetate or formate, and the protein profiles were compared by two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Acetate increased the steady-state expression levels of 37 proteins, including periplasmic transporters for amino acids and peptides (ArtI, FliY, OppA, and ProX), metabolic enzymes (YfiD and GatY), the RpoS growth phase regulon, and the autoinducer synthesis protein LuxS. Acetate repressed 17 proteins, among them phosphotransferase (Pta). An ackA-pta deletion, which nearly eliminates interconversion between acetate and acetyl-coenzyme A (acetyl-CoA), led to elevated basal levels of 16 of the acetate-inducible proteins, including the RpoS regulon. Consistent with RpoS activation, the ackA-pta strain also showed constitutive extreme-acid resistance. Formate, however, repressed 10 of the acetate-inducible proteins, including the RpoS regulon. Ten of the proteins with elevated basal levels in the ackA-ptastrain were repressed by growth of the mutant with formate; thus, the formate response took precedence over the loss of theackA-pta pathway. The similar effects of exogenous acetate and the ackA-pta deletion, and the opposite effect of formate, could have several causes; one possibility is that the excess buildup of acetyl-CoA upregulates stress proteins but excess formate depletes acetyl-CoA and downregulates these proteins.

scholarly journals From Eat to trEat: engineering the mitochondrial Eat1 enzyme for enhanced ethyl acetate production in Escherichia coli

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Aleksander J. Kruis ◽  
Anna C. Bohnenkamp ◽  
Bram Nap ◽  
Jochem Nielsen ◽  
Astrid E. Mars ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Ethyl Acetate ◽  
Acetate Production

scholarly journals Multilevel optimisation of anaerobic ethyl acetate production in engineered Escherichia coli

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Anna C. Bohnenkamp ◽  
Aleksander J. Kruis ◽  
Astrid E. Mars ◽  
Rene H. Wijffels ◽  
John van der Oost ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Ethyl Acetate ◽  
Acetate Production ◽  
Engineered Escherichia Coli ◽  
Multilevel Optimisation

Acetyl-CoA-Dependent Chain Elongation of Fatty Acids in Escherichia coli K-121

1986 ◽  
Vol 99 (2) ◽  
pp. 365-374
Author(s):  
Tomoko NISHIMAKI ◽  
Hiroshi YAMANAKA ◽  
Michinao MIZUGAKI
Keyword(s):  
Escherichia Coli ◽  
Fatty Acids ◽  
Chain Elongation ◽  
Acetyl Coa
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