Broth Recycling To Reduce Process Noise Resulting from Concentrated Substrate Addition in Fed-Batch Cultivation of Escherichia coli

2003 ◽  
Vol 19 (2) ◽  
pp. 659-661 ◽  
Author(s):  
W.A. Johnston ◽  
M.J. Cooney
Keyword(s):  
Escherichia Coli ◽  
Batch Cultivation ◽  
Fed Batch ◽  
Process Noise ◽  
Fed Batch Cultivation ◽  
Substrate Addition

scholarly journals De novo Biosynthesis of Biodiesel by Escherichia coli in Optimized Fed-Batch Cultivation

PLoS ONE ◽  
2011 ◽  
Vol 6 (5) ◽  
pp. e20265 ◽  
Author(s):  
Yangkai Duan ◽  
Zhi Zhu ◽  
Ke Cai ◽  
Xiaoming Tan ◽  
Xuefeng Lu
Keyword(s):  
Escherichia Coli ◽  
De Novo ◽  
Batch Cultivation ◽  
Fed Batch ◽  
De Novo Biosynthesis ◽  
Fed Batch Cultivation

scholarly journals Fed-Batch Cultivation and Adding Supplements to Increase Yield of β-1,3-1,4-Glucanase by Genetically Engineered Escherichia coli

Catalysts ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 269
Author(s):  
Lijuan Zhong ◽  
Zheng Liu ◽  
Yinghua Lu
Keyword(s):  
Escherichia Coli ◽  
Feeding Strategy ◽  
Batch Cultivation ◽  
Genetically Engineered ◽  
Fed Batch ◽  
Glucanase Activity ◽  
E Coli ◽  
Constant Rate ◽  
Engineered Escherichia Coli ◽  
Fed Batch Cultivation

The aim of this study was to analyze the major influence factors of culture medium on the expression level of β-1,3-1,4-glucanase, and to further develop an optimized process for the extracellular production of β-glucanase at a bioreactor scale (7 L) with a genetically engineered Escherichia coli (E. coli) JM109-pLF3. In this study, batch cultivation and fed-batch cultivation including the constant rate feeding strategy and the DO-stat (DO: Dissolved Oxygen) feeding strategy were conducted. At a 7 L bioreactor scale for batch cultivation, biomass reached 3.14 g/L and the maximum β-glucanase activity was 506.94 U/mL. Compared with batch cultivation, the addition of glycerol, complex nitrogen and complete medium during fed-batch cultivation increased the production of biomass and β-1,3-1,4-glucanase. The maximum biomass and β-glucanase activity, which were 7.67 g/L and 1680 U/mL, respectively, that is, 2.45 and 3.31 times higher than those obtained with batch cultivation, were obtained by feeding a complex nitrogen source at a constant rate of 1.11 mL/min. Therefore, these nutritional supplements and strategies can be used as a reference to enhance the production of other bioproducts from E. coli.

Sign in / Sign up

Export Citation Format

Share Document