Improved Cell Growth in Tobacco Suspension Cultures Expressing Vitreoscilla Hemoglobin†

2002 ◽  
Vol 18 (2) ◽  
pp. 229-233 ◽  
Author(s):  
J. Farres ◽  
P.T. Kallio
Keyword(s):  
Cell Growth ◽  
Suspension Cultures ◽  
Vitreoscilla Hemoglobin ◽  
Tobacco Suspension Cultures

scholarly journals Flavonoid Accumulation in Cell Suspension Cultures of Glycyrrhiza inflata Batal under Optimizing Conditions

2009 ◽  
Vol 64 (1-2) ◽  
pp. 68-72 ◽  
Author(s):  
Ying Yang ◽  
Feng He ◽  
Longjiang Yu ◽  
Jiaxing Ji ◽  
Yezhen Wang
Keyword(s):  
Cell Growth ◽  
Cell Suspension ◽  
Cell Cultures ◽  
Large Scale ◽  
Cell Suspension Cultures ◽  
Optimization Method ◽  
Suspension Cultures ◽  
Initial Inoculum ◽  
Nitrogen Concentrations ◽  
Glycyrrhiza Inflata

Cell growth and flavonoid production in cell suspension cultures of Glycyrrhiza inflata Batal were investigated under various initial inoculum densities, and sucrose and nitrogen concentrations to develop an optimization method for an improved flavonoid production. Both biomass accumulation and flavonoid production exhibited an “S” curve in one culture cycle, with the greatest value obtained on day 21, which showed that cell growth and fl avonoid biosynthesis went along isochronously. Moreover, according to the biomass and flavonoid production, the appreciate inoculum density, and the sucrose and nitrogen concentrations were 50 g FW L-1, 50 g L-1 and 120 mmol L-1, respectively. In addition, cell growth and flavonoid production showed a peak of 16.4 g DW L-1 and 95.7 mg L-1 on day 21 under the optimizing conditions, respectively. The flavonoid productivity of the cells which were cultured for 3 years is higher than that of the 3-year-old plant, which suggested that flavonoid production by cell cultures of G. inflata is a potentially profitable method. Therefore, this work is considered to be helpful for efficient large-scale bioprocessing of cell cultures in bioreactors.

Improvement of Amorpha-4,11-diene Production by a Yeast-Conform Variant of Vitreoscilla Hemoglobin

2012 ◽  
Vol 67 (3-4) ◽  
pp. 195-207 ◽  
Author(s):  
Junhao Shen ◽  
Haina Zheng ◽  
Xiaohui Zhi ◽  
Yanwei Shi ◽  
Yong Huang ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Growth ◽  
Western Blot ◽  
Difference Spectrum ◽  
Vitreoscilla Hemoglobin ◽  
Expression Vectors ◽  
Chain Reactions ◽  
Polymerase Chain Reactions ◽  
Polymerase Chain ◽  
Engineered Saccharomyces Cerevisiae

Amorpha-4,11-diene is the precursor of the antimalarial compound artemisinin. The effect of Vitreoscilla hemoglobin (VHb) and its yeast-conform variant (VHbm) on amorpha-4,11- diene production in engineered Saccharomyces cerevisiae was investigated. First, the VHb gene was mutated to the yeast-conform variant VHbm based on step-by-step extension of a short region of the gene through a series of polymerase chain reactions (PCR). The artificial VHbm gene contained codons preferred by the yeast translation machinery. Two yeast expression vectors containing VHb or VHbm gene were constructed and introduced into the amorpha-4,11-diene-producing strain S. cerevisiae WK1 to form WK1[VHb] and WK1[VHbm], respectively. Western blot and CO-difference spectrum absorbance assay showed that VHb and VHbm were successfully expressed. In shake fl asks, VHbm expression conferred higher cell growth than VHb expression. GC-MS results indicated the amorpha- 4,11-diene production in WK1[VHbm] and WK1[VHb] was 3- and 2-fold higher than that in WK1, respectively. This suggests that VHb might improve the amorpha-4,11-diene production in engineered S. cerevisiae

Effect of carbon dioxide on cell growth and saponin production in suspension cultures of Panax ginseng

2006 ◽  
Vol 50 (4) ◽  
pp. 752-754 ◽  
Author(s):  
N. T. Thanh ◽  
H. N. Murthy ◽  
D. M. Pandey ◽  
K. W. Yu ◽  
E. J. Hahn ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Cell Growth ◽  
Panax Ginseng ◽  
Suspension Cultures

Evidence for the Participation of a 5-Oxo-prolinase in Degradation of Glutathione in Nicotiana tabacum

1980 ◽  
Vol 35 (9-10) ◽  
pp. 708-711 ◽  
Author(s):  
Heinz Rennenberg ◽  
Reinhard Steinkamp ◽  
Andrea Polle
Keyword(s):  
Nicotiana Tabacum ◽  
Glutamic Acid ◽  
Suspension Cultures ◽  
Sulfur Source ◽  
Tobacco Cells ◽  
Tobacco Suspension Cultures

Abstract During degradation of glutathione in tobacco suspension cultures substancial amounts of 5-oxo-proline are formed in vivo as well as in crude cell homogenates in vitro. The existance of a 5-oxo-prolinase that catalyzes the conversion of 5-oxo-proline to glutamic acid was demonstrated in tobacco cells, grown with glutathione as sole sulfur source.

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