Two-Stage Culture Process for Improved Production of Ganoderic Acid by Liquid Fermentation of Higher Fungus Ganoderma lucidum

2002 ◽  
Vol 18 (1) ◽  
pp. 51-54 ◽  
Author(s):  
Q.-H. Fang ◽  
J.-J. Zhong
Keyword(s):  
Ganoderma Lucidum ◽  
Ganoderic Acid ◽  
Two Stage ◽  
Liquid Fermentation ◽  
Culture Process ◽  
Two Stage Culture

Hybrid two-stage culture of Halamphora coffeaeformis for biodiesel production: Growth phases, nutritional stages and biorefinery approach

2018 ◽  
Vol 118 ◽  
pp. 984-992 ◽  
Author(s):  
Lucas A. Martín ◽  
Cecilia A. Popovich ◽  
Ana M. Martínez ◽  
Paola G. Scodelaro Bilbao ◽  
María C. Damiani ◽  
...  
Keyword(s):  
Biodiesel Production ◽  
Growth Phases ◽  
Two Stage ◽  
Production Growth ◽  
Two Stage Culture

scholarly journals Optimization of Culture Condition for Ganoderic Acid Production in Ganoderma lucidum Liquid Static Culture and Design of a Suitable Bioreactor

Molecules ◽  
2018 ◽  
Vol 23 (10) ◽  
pp. 2563 ◽  
Author(s):  
Gaosheng Hu ◽  
Manhuayun Zhai ◽  
Rong Niu ◽  
Xiaoqiang Xu ◽  
Qian Liu ◽  
...  
Keyword(s):  
Ganoderma Lucidum ◽  
Liquid Culture ◽  
Management Practices ◽  
Total Yield ◽  
Gas Content ◽  
Market Demand ◽  
Ganoderic Acid ◽  
Static Culture ◽  
Air Supply ◽  
Liquid Static Culture

Ganoderma lucidum, a famous medicinal mushroom used worldwide, is a rich source of triterpenoids which, together with polysaccharides, are believed to be the main effective constituents of G. lucidum. With the increase of market demand, the wild resource is facing serious limitations, and the quality of cultivated fruiting bodies can be seriously affected by the availability of wood resources and by cultivation management practices. In the present study, we aimed to develop an alternative way to produce useful triterpenoids from G. lucidum. We cultured the strain using a two-stage liquid culture strategy and investigated the effects of nitrogen limitation, carbon supply, static culture volume and air supply in the static culture stage on the accumulation of five triterpenoids (GA-P, GA-Q, GA-T, GA-S, GA-R). Our results showed that, under optimized condition, the total yield of the five triterpenoids reached 963 mg/L (as determined by HPLC). Among the five triterpenoids, GA-T accounted for about 75% of the total yield. Besides, a bioreactor suitable for fungal liquid static culture with a 10 L extensible plastic bag shaped culture unit was designed and in which the maximum total yield of the five GAs reached 856.8 mg/L, and the GAs content reached 5.99%. Our results demonstrate the potential of industrial application of G. lucidum culture for the production of triterpenoids, especially GA-T. Air supply significantly improved the accumulation of triterpenoids, and this will provide important clues to understand why more triterpenoids are produced in the mycelia mat under static liquid culture conditions.

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