Whole-Cell Immobilization Using Cell Surface-Exposed Cellulose-Binding Domain

2001 ◽  
Vol 17 (3) ◽  
pp. 407-411 ◽  
Author(s):  
A.A. Wang ◽  
A. Mulchandani ◽  
W. Chen
Keyword(s):  
Cell Surface ◽  
Cell Immobilization ◽  
Binding Domain ◽  
Cellulose Binding Domain ◽  
Whole Cell ◽  
Cellulose Binding

scholarly journals Specific Adhesion to Cellulose and Hydrolysis of Organophosphate Nerve Agents by a Genetically Engineered Escherichia coli Strain with a Surface-Expressed Cellulose-Binding Domain and Organophosphorus Hydrolase

2002 ◽  
Vol 68 (4) ◽  
pp. 1684-1689 ◽  
Author(s):  
Aijun A. Wang ◽  
Ashok Mulchandani ◽  
Wilfred Chen
Keyword(s):  
Escherichia Coli ◽  
Cell Surface ◽  
Nerve Agents ◽  
Genetically Engineered ◽  
Binding Domain ◽  
Organophosphorus Hydrolase ◽  
Fusion System ◽  
Cellulose Binding Domain ◽  
Cellulose Binding ◽  
Hydrolysis Of

ABSTRACT A genetically engineered Escherichia coli cell expressing both organophosphorus hydrolase (OPH) and a cellulose-binding domain (CBD) on the cell surface was constructed, enabling the simultaneous hydrolysis of organophosphate nerve agents and immobilization via specific adsorption to cellulose. OPH was displayed on the cell surface by use of the truncated ice nucleation protein (INPNC) fusion system, while the CBD was surface anchored by the Lpp-OmpA fusion system. Production of both INPNC-OPH and Lpp-OmpA-CBD fusion proteins was verified by immunoblotting, and the surface localization of OPH and the CBD was confirmed by immunofluorescence microscopy. Whole-cell immobilization with the surface-anchored CBD was very specific, forming essentially a monolayer of cells on different supports, as shown by electron micrographs. Optimal levels of OPH activity and binding affinity to cellulose supports were achieved by investigating expression under different induction levels. Immobilized cells degraded paraoxon rapidly at an initial rate of 0.65 mM/min/g of cells (dry weight) and retained almost 100% efficiency over a period of 45 days. Owing to its superior degradation capacity and affinity to cellulose, this immobilized-cell system should be an attractive alternative for large-scale detoxification of organophosphate nerve agents.

scholarly journals Cellulose as an inert matrix for presenting cytokines to target cells: production and properties of a stem cell factor–cellulose-binding domain fusion protein

1999 ◽  
Vol 339 (2) ◽  
pp. 429-434
Author(s):  
J. Greg DOHENY ◽  
Eric J. JERVIS ◽  
M. Marta GUARNA ◽  
R. Keith HUMPHRIES ◽  
R. Antony J. WARREN ◽  
...  
Keyword(s):  
Stem Cell ◽  
Fusion Protein ◽  
Stem Cell Factor ◽  
Direct Analysis ◽  
Binding Domain ◽  
Target Cells ◽  
Cellulose Binding Domain ◽  
Binding Characteristics ◽  
Cellulose Binding ◽  
Cellulose Surface

A chimaera of stem cell factor (SCF) and a cellulose-binding domain from the xylanase Cex (CBDCex) effectively immobilizes SCF on a cellulose surface. The fusion protein retains both the cytokine properties of SCF and the cellulose-binding characteristics of CBDCex. When adsorbed on cellulose, SCF–CBDCex is up to 7-fold more potent than soluble SCF–CBDCex and than native SCF at stimulating the proliferation of factor-dependent cell lines. When cells are incubated with cellulose-bound SCF–CBDCex, activated receptors and SCF–CBDCex co-localize on the cellulose matrix. The strong binding of SCF–CBDCex to the cellulose surface permits the effective and localized stimulation of target cells; this is potentially significant for long-term perfusion culturing of factor-dependent cells. It also permits the direct analysis of the effects of surface-bound cytokines on target cells.

scholarly journals Fusion Endoglucanase Cel12B from Thermotoga maritima with Cellulose Binding Domain

BioResources ◽  
2018 ◽  
Vol 13 (2) ◽  
Author(s):  
Hao Shi ◽  
Yanling Chen ◽  
Wenjian Peng ◽  
Pixiang Wang ◽  
Yuping Zhao ◽  
...  
Keyword(s):  
Thermotoga Maritima ◽  
Binding Domain ◽  
Cellulose Binding Domain ◽  
Cellulose Binding

scholarly journals Acetyl xylan esterase II fromPenicillium purpurogenumis similar to an esterase fromTrichoderma reeseibut lacks a cellulose binding domain

FEBS Letters ◽  
1998 ◽  
Vol 423 (1) ◽  
pp. 35-38 ◽  
Author(s):  
Rodrigo Gutiérrez ◽  
Ella Cederlund ◽  
Lars Hjelmqvist ◽  
Alessandra Peirano ◽  
Francisco Herrera ◽  
...  
Keyword(s):  
Binding Domain ◽  
Cellulose Binding Domain ◽  
Acetyl Xylan Esterase ◽  
Cellulose Binding

Fabrication of cotton fabrics using family III cellulose-binding domain for enhanced surface properties

RSC Advances ◽  
2016 ◽  
Vol 6 (107) ◽  
pp. 105202-105205 ◽  
Author(s):  
Liting Zhang ◽  
Yaofei Sun ◽  
Wenji Yao ◽  
Guoying Dai ◽  
Ping Wang
Keyword(s):  
Surface Properties ◽  
Cotton Fabric ◽  
Surface Functionalization ◽  
Physical Adsorption ◽  
Cotton Fabrics ◽  
Binding Domain ◽  
Cellulose Binding Domain ◽  
Fabric Surface ◽  
Cellulose Binding ◽  
Enhanced Surface

Cotton fabric surface functionalization by physical adsorption of CBDIII through a sample soaking process.

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