Evaluation of UNIFAC for the Prediction of Partitioning in Two-Phase Aqueous-Organic Systems Containing Amino Acids and Oligomers

1995 ◽  
Vol 11 (6) ◽  
pp. 704-707 ◽  
Author(s):  
Jason D. Roberts ◽  
Andrew J. Daugulis
Author(s):  
Mos van Berlo ◽  
Marcel Ottens ◽  
Karel Ch.A.M Luyben ◽  
Luuk A.M van der Wielen

RSC Advances ◽  
2014 ◽  
Vol 4 (90) ◽  
pp. 49485-49490 ◽  
Author(s):  
C. D. M. Campos ◽  
J. K. Park ◽  
P. Neužil ◽  
J. A. F. da Silva ◽  
A. Manz

We present a method of continuous electroextraction of amino acids using aqueous two phase system in a microchip. The separations occur due to differences in electrophoretic mobility and solvent affinity. The results suggest the possibility of high levels of purification by controlling the electric field across the liquid barrier.


Author(s):  
Emmanuel Chiwo Omondi ◽  
Marisa Wagner ◽  
Atanu Mukherjee ◽  
Kristine Nichols

Abstract Declining nutrient densities of crops in the past 50–70 years have been attributed to unsound agricultural practices and plant breeding focus on yield rather than quality. Few studies have quantified the soil and nutritional quality of grains in organic and conventional farms and reported results are scarce and inconsistent. The Rodale Institute's Farming Systems Trial (FST) was established in 1981 to quantify the effects of long-term organic and conventional grain cropping systems and tillage practices. A 2014 study to quantify effects on the nutrient density of oat grains was integrated into three systems within the long-term trial: organic manure-based (MNR), organic legume-based (LEG), and conventional synthetic input-based (CNV), split between tilled (T) and no-till (NT) practices. Oat grains with hulls removed were analyzed for minerals (n = 24), vitamins (n = 24), amino acids (n = 24) and proteins (n = 24), while soil samples to a depth of 10 cm were analyzed for elemental minerals, and total carbon (C), nitrogen (N) and sulfur (S). Organic systems increased six out ten soil minerals whose concentrations were influenced by cropping systems: aluminum (Al), iron (Fe), chromium (Cr), calcium (Ca), barium (B) and strontium (Sr). All essential amino acids were greater in oat grains under LEG systems compared with other systems except lysine, histidine and methionine. Both LEG systems also increased 12 out of 13 non-essential amino acids in oat grains. Total oat N, C and S required for amino acid synthesis tended to be greater in organic systems. Soil N, C and S were highly correlated with total oat amino acids under organic systems compared to CNV. Organic LEG had significantly greater vitamin B1 than MNR and CNV. These results suggest that nutrient concentrations of oat grains were greater in organic systems compared to CNV systems, and the increase could be partially explained by the long-term soil management differences between the systems.


AMB Express ◽  
2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Sebastian Oppermann ◽  
Christina Oppermann ◽  
Miriam Böhm ◽  
Toni Kühl ◽  
Diana Imhof ◽  
...  

2014 ◽  
Vol 1329 ◽  
pp. 52-60 ◽  
Author(s):  
Dragana P.C. de Barros ◽  
Sara R.R. Campos ◽  
Pedro P. Madeira ◽  
Ana M. Azevedo ◽  
António M. Baptista ◽  
...  

2010 ◽  
Vol 104 (8) ◽  
pp. 1174-1180 ◽  
Author(s):  
Asha Badaloo ◽  
Jean W.-C. Hsu ◽  
Carolyn Taylor-Bryan ◽  
Marvin Reid ◽  
Terrence Forrester ◽  
...  

The requirement for aromatic amino acids during the rapid catch-up in weight phase of recovery from severe childhood undernutrition (SCU) is not clearly established. As a first step, the present study aimed to estimate the tyrosine requirement of children with SCU during the catch-up growth phase of nutritional rehabilitation using a diet enriched in energy and proteins. Tyrosine requirement was calculated from the rate of excretion of 13CO2 (F 13CO2) during [13C]phenylalanine infusion in thirteen children with SCU, five females and eight males, at about 19 d after admission when the subjects were considered to have entered their rapid catch-up growth phase and were consuming 627·3 kJ and about 3·5 g protein/kg per d. Measurements of F 13CO2 during [13C]phenylalanine infusion were made on two separate days with a 1 d interval. Three measurements at tyrosine intakes of 48, 71 and 95 mg/kg per d were performed on experimental day 1 and measurements at tyrosine intakes of 148, 195 and 241 mg/kg per d were performed on experimental day 2. An estimate of the mean requirement was derived by breakpoint analysis with a two-phase linear regression cross-over model. The breakpoint, which represents an estimate of the mean tyrosine requirement, is a value of 99 mg/kg per d when the children were growing at about 15 g/kg per d. The result indicates that the mean requirement for tyrosine during the catch-up growth phase of SCU is about 99 mg/kg per d under similar conditions to the present study.


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