Partial and total cell retention in a filtration-based homogeneous perfusion reactor

1995 ◽  
Vol 11 (5) ◽  
pp. 584-588 ◽  
Author(s):  
Gautam G. Banik ◽  
Carole A. Heath
Keyword(s):  
Total Cell ◽  
Cell Retention

Continuous ethanol production from wood hydrolysate by chemostat and total cell retention culture

1996 ◽  
Vol 13 (5) ◽  
pp. 453-456 ◽  
Author(s):  
Woo Gi Lee ◽  
Jin Suk Lee ◽  
Byung Geon Park ◽  
Mi Sun Kim ◽  
Soon Chul Park ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Total Cell ◽  
Cell Retention ◽  
Wood Hydrolysate

Metabolic response of Bacillus sphaericus 1593M for dual-substrate limitation in continuous and total-cell-retention cultures

1997 ◽  
Vol 47 (5) ◽  
pp. 554-559 ◽  
Author(s):  
S. Meenakshisundaram ◽  
G. Suresh ◽  
R. K. Fernando ◽  
K. Jenny ◽  
R. Sachidanandham ◽  
...  
Keyword(s):  
Metabolic Response ◽  
Bacillus Sphaericus ◽  
Total Cell ◽  
Cell Retention ◽  
Substrate Limitation ◽  
Dual Substrate Limitation ◽  
Dual Substrate

Improved Production of Bacillus thuringiensis by Intermittent Fed-Batch Culture with Total Cell Retention

2010 ◽  
Vol 8 (1) ◽  
Author(s):  
Edwin Habeych ◽  
Andrés F González Barrios ◽  
Alexandre Restrepo-Boland ◽  
Sergio Orduz
Keyword(s):  
Bacillus Thuringiensis ◽  
Batch Culture ◽  
Spodoptera Frugiperda ◽  
Total Cell ◽  
Spore Concentration ◽  
Fed Batch ◽  
Cell Retention ◽  
Filtration System ◽  
Fed Batch Culture ◽  
Flow Filtration

Intermittent fed-batch culture with total cell retention was evaluated during Bacillus thuringiensis serovar. kurstaki fermentations aiming to enhance biomass and spore concentration yields while maintaining toxicity. This method is derived from the already reported intermittent fed-batch culture and utilized a tangential flow filtration system for cell retention. After 4 pulses, we obtained a biomass of 74.36 g·L-1 and this biomass displayed a half lethal concentration towards neonate Spodoptera frugiperda larvae of 40.53 ng·cm-2, and spore concentration of 4.6 x 1014 CFU mL-1. On the other hand, volumetric productivity was increased seven-fold compared to batch fermentation. Interestingly, we are not aware of higher spore concentrations reported to date for B. thuringiensis cultures.

Rapid and reliable preparation of total cell lysates from fission yeast

2006 ◽  
Author(s):  
Atsuko Shirai ◽  
Akihisa Matsuyama ◽  
Yoko Yashiroda ◽  
Ritsuko Arai ◽  
Minoru Yoshida
Keyword(s):  
Fission Yeast ◽  
Total Cell ◽  
Cell Lysates

MICROBES PARAMETERS OF WATER QUALITY FOR AQUACULTURE ON PARI ISLAND WATERS

2013 ◽  
Vol 5 (1) ◽  
Author(s):  
Lies Indah Sutiknowati
Keyword(s):  
Pathogenic Bacteria ◽  
Heterotrophic Bacteria ◽  
Total Coliform ◽  
Total Cell ◽  
Epifluorescence Microscopy ◽  
Coliform Bacteria ◽  
Total Density ◽  
Shellfish Aquaculture ◽  
Microbiological Parameters ◽  
Phosphate Nitrate

The objective of this research was to evaluate waters quality in Pari island waters for aquaculture purpose based on bacteriological information conducted in Mei and September 2011. Microbiological parameters analyzed were total density of bacteria for coliforms, E.coli, pathogenic, heterotrophic, halotoleran, phosphate-nitrate-ammonia breaker, and total cells. Method to analyze coliform bacteria was filtration, identification of pathogenic bacteria using biochemical test, density analises for heterotrophic bacteria, analises for phosphate-nitrate-ammonia breaker bacteria using pour plate, and total cell using Acridine Orange Epifluorescence Microscopy. Results showed that the abundance of total coliform cell was about 1000-7000 colony forming unit (cfu)/100 ml. The abundance of heterotrophic, halotolerant, phosphate-nitrate-ammonia bacteria in seawater was (3.6-4.3)x105 cfu/ml, (1.1-1.3)x105 cfu/ml, (0.5-3.44)x103cfu/ml; and (3.6-6.7)x105 cfu/ml, (1.6-2.7)x105 cfu/ml, (0.6-5.22)x103 cfu/ml in sediment, respectively. The total cell of bacteria was (0.05-2.1)x107cells/ml. The dog-conch (Strombus turturella) and blood-clamps (Anadara granosa) can survive in Pari Island and there was a significant increase in sea grass litter with growth average of 0.67 mm/day and 0.90 mm/day. During snails and clamps growth, there were found several genus of pathogenic bacteria such as Salmonella, Vibrio, Aeromonas, Pseudomonas, Citrobacter, Proteus, Shigella, Hafnia, and Yersinia. The results showed that Pari island waters was suitable for developing shellfish aquaculture dog conch and blood clamps. Keywords: bacteria, parameter, shellfish, aquaculture.

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