A model for .beta.-galactosidase production with a recombinant yeast Saccharomyces cerevisiae in fed-batch culture

1992 ◽  
Vol 8 (4) ◽  
pp. 298-306 ◽  
Author(s):  
Linawati Hardjito ◽  
Paul F. Greenfield ◽  
Peter L. Lee
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Batch Culture ◽  
Recombinant Yeast ◽  
Fed Batch ◽  
Yeast Saccharomyces Cerevisiae ◽  
Fed Batch Culture ◽  
Beta Galactosidase

scholarly journals A cytofluorimetric analysis of a Saccharomyces cerevisiae population cultured in a fed-batch bioreactor

2021 ◽  
Author(s):  
Massimo Sanchez ◽  
Emanuela Palomba ◽  
Valentina Tirelli ◽  
Elisabetta de Alteriis ◽  
Carmine Landi ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Cycle ◽  
Batch Culture ◽  
Time Course ◽  
Reference Model ◽  
Product Yield ◽  
Fed Batch ◽  
Yeast Saccharomyces Cerevisiae ◽  
Biotechnological Processes ◽  
Fed Batch Culture

The yeast Saccharomyces cerevisiae is a reference model system and one of the widely used microorganisms in many biotechnological processes. In industrial yeast applications, combined strategies aim to maximize biomass/product yield, with the fed-batch culture being one of the most frequently used. Flow cytometry (FCM) is widely applied in biotechnological processes and represents a key methodology to monitor cell population dynamics. We propose here an application of FCM in the analysis of yeast cell cycle along the time course of a typical S. cerevisiae fed-batch culture. We used two different dyes, SYTOX Green and SYBR Green, with the aim to better define each stage of cell cycle during S. cerevisiae fed-batch culture. The results provide novel insights in the use of FCM cell cycle analysis for the real-time monitoring of S. cerevisiae bioprocesses.

Production of Hepatitis B Surface Antigen Including PreS (PreS-HBsAg) in Fed-Batch Culture of Recombinant Yeast

1992 ◽  
pp. 186-188
Author(s):  
Joong-Chul Lee ◽  
Sung Jin Kim ◽  
Eui-Cheol Jo ◽  
Dong-Il Kim ◽  
Kyung Hwan Jung ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Batch Culture ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Recombinant Yeast ◽  
Fed Batch ◽  
Fed Batch Culture

scholarly journals A cytofluorimetric analysis of a Saccharomyces cerevisiae population cultured in a fed-batch bioreactor

PLoS ONE ◽  
2021 ◽  
Vol 16 (6) ◽  
pp. e0248382
Author(s):  
Emanuela Palomba ◽  
Valentina Tirelli ◽  
Elisabetta de Alteriis ◽  
Palma Parascandola ◽  
Carmine Landi ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Cycle ◽  
Batch Culture ◽  
Time Course ◽  
Reference Model ◽  
Product Yield ◽  
Fed Batch ◽  
Batch Bioreactor ◽  
Biotechnological Processes ◽  
Fed Batch Culture

The yeast Saccharomyces cerevisiae is a reference model system and one of the widely used microorganisms in many biotechnological processes. In industrial yeast applications, combined strategies aim to maximize biomass/product yield, with the fed-batch culture being one of the most frequently used. Flow cytometry (FCM) is widely applied in biotechnological processes and represents a key methodology to monitor cell population dynamics. We propose here an application of FCM in the analysis of yeast cell cycle along the time course of a typical S. cerevisiae fed-batch culture. We used two different dyes, SYTOX Green and SYBR Green, with the aim to better define each stage of cell cycle during S. cerevisiae fed-batch culture. The results provide novel insights in the use of FCM cell cycle analysis for the real-time monitoring of S. cerevisiae bioprocesses.

Glutathione accumulation in ethanol-stat fed-batch culture of Saccharomyces cerevisiae with a switch to cysteine feeding

2010 ◽  
Vol 87 (1) ◽  
pp. 175-183 ◽  
Author(s):  
Ildar Nisamedtinov ◽  
Kaspar Kevvai ◽  
Kerti Orumets ◽  
Jari J. Rautio ◽  
Toomas Paalme
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Batch Culture ◽  
Fed Batch ◽  
Fed Batch Culture
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