Comparisons of the Glycosylation of a Monoclonal Antibody Produced under Nominally Identical Cell Culture Conditions in Two Different Bioreactors

2000 ◽  
Vol 16 (3) ◽  
pp. 462-470 ◽  
Author(s):  
J.P. Kunkel ◽  
D.C.H. Jan ◽  
M. Butler ◽  
J.C. Jamieson
Keyword(s):  
Cell Culture ◽  
Monoclonal Antibody ◽  
Culture Conditions ◽  
Identical Cell ◽  
Cell Culture Conditions

A semi-empirical glycosylation model of a camelid monoclonal antibody under hypothermia cell culture conditions

2017 ◽  
Vol 44 (7) ◽  
pp. 1005-1020 ◽  
Author(s):  
Hengameh Aghamohseni ◽  
Maureen Spearman ◽  
Kaveh Ohadi ◽  
Katrin Braasch ◽  
Murray Moo-Young ◽  
...  
Keyword(s):  
Cell Culture ◽  
Monoclonal Antibody ◽  
Culture Conditions ◽  
Semi Empirical ◽  
Cell Culture Conditions

scholarly journals Cell culture-based profiling across mammals reveals DNA repair and metabolism as determinants of species longevity

eLife ◽  
2016 ◽  
Vol 5 ◽  
Author(s):  
Siming Ma ◽  
Akhil Upneja ◽  
Andrzej Galecki ◽  
Yi-Miau Tsai ◽  
Charles F Burant ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Culture ◽  
Dna Repair ◽  
Protein Transport ◽  
Global Gene Expression ◽  
Culture Conditions ◽  
Identical Cell ◽  
Species Specific ◽  
Cell Culture Conditions ◽  
Species Longevity

Mammalian lifespan differs by >100 fold, but the mechanisms associated with such longevity differences are not understood. Here, we conducted a study on primary skin fibroblasts isolated from 16 species of mammals and maintained under identical cell culture conditions. We developed a pipeline for obtaining species-specific ortholog sequences, profiled gene expression by RNA-seq and small molecules by metabolite profiling, and identified genes and metabolites correlating with species longevity. Cells from longer lived species up-regulated genes involved in DNA repair and glucose metabolism, down-regulated proteolysis and protein transport, and showed high levels of amino acids but low levels of lysophosphatidylcholine and lysophosphatidylethanolamine. The amino acid patterns were recapitulated by further analyses of primate and bird fibroblasts. The study suggests that fibroblast profiling captures differences in longevity across mammals at the level of global gene expression and metabolite levels and reveals pathways that define these differences.

scholarly journals Electrochemical Stability of Poly(3,4-Ethylenedioxythiophene) Derivatives Under Cell Culture Conditions

2021 ◽  
Vol 1885 (3) ◽  
pp. 032004
Author(s):  
Qichao Pan ◽  
Zuwei Zhang ◽  
Yaqiong Zhang ◽  
Yaopeng Zhang ◽  
Bo Zhu
Keyword(s):  
Cell Culture ◽  
Culture Conditions ◽  
Electrochemical Stability ◽  
Cell Culture Conditions

Influence of cell culture conditions on aromatase activity in human genital skin fibroblasts

1989 ◽  
Vol 25 (9) ◽  
pp. 806-812 ◽  
Author(s):  
Tarek Bisat ◽  
Terry R. Brown ◽  
Claude J. Migeon ◽  
Gary D. Berkovitz
Keyword(s):  
Cell Culture ◽  
Culture Conditions ◽  
Skin Fibroblasts ◽  
Aromatase Activity ◽  
Cell Culture Conditions

scholarly journals Glioma cells showing IDH1 mutation cannot be propagated in standard cell culture conditions

2011 ◽  
Vol 104 (6) ◽  
pp. 968-970 ◽  
Author(s):  
S Piaskowski ◽  
M Bienkowski ◽  
E Stoczynska-Fidelus ◽  
R Stawski ◽  
M Sieruta ◽  
...  
Keyword(s):  
Cell Culture ◽  
Glioma Cells ◽  
Culture Conditions ◽  
Idh1 Mutation ◽  
Standard Cell ◽  
Cell Culture Conditions
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