scholarly journals Reversibility of β-Amyloid Self-Assembly: Effects of pH and Added Salts Assessed by Fluorescence Photobleaching Recovery

2010 ◽  
Vol 11 (2) ◽  
pp. 341-347 ◽  
Author(s):  
Nadia J. Edwin ◽  
Robert P. Hammer ◽  
Robin L. McCarley ◽  
Paul S. Russo
Keyword(s):  
Self Assembly ◽  
Fluorescence Photobleaching Recovery ◽  
Photobleaching Recovery ◽  
Effects Of Ph ◽  
Β Amyloid

What do nanometer molecular trajectories tell us about heterogeneous cell surface domaines?

1995 ◽  
Vol 53 ◽  
pp. 786-787
Author(s):  
Watt W. Webb
Keyword(s):  
Cell Surface ◽  
Lipid Membranes ◽  
Surface Proteins ◽  
Protein Diffusion ◽  
Coated Pits ◽  
Cell Surface Proteins ◽  
Membrane Heterogeneity ◽  
Fluorescence Photobleaching Recovery ◽  
Photobleaching Recovery ◽  
Plasma Membrane Heterogeneity

Plasma membrane heterogeneity is implicit in the existence of specialized cell surface organelles which are necessary for cellular function; coated pits, post and pre-synaptic terminals, microvillae, caveolae, tight junctions, focal contacts and endothelial polarization are examples. The persistence of these discrete molecular aggregates depends on localized restraint of the constituent molecules within specific domaines in the cell surface by strong intermolecular bonds and/or anchorage to extended cytoskeleton. The observed plasticity of many of organelles and the dynamical modulation of domaines induced by cellular signaling evidence evanescent intermolecular interactions even in conspicuous aggregates. There is also strong evidence that universal restraints on the mobility of cell surface proteins persist virtually everywhere in cell surfaces, not only in the discrete organelles. Diffusion of cell surface proteins is slowed by several orders of magnitude relative to corresponding protein diffusion coefficients in isolated lipid membranes as has been determined by various ensemble average methods of measurement such as fluorescence photobleaching recovery(FPR).

Photosensitizing materials and platforms for light-triggered modulation of Alzheimer's β-amyloid self-assembly

Biomaterials ◽  
2019 ◽  
Vol 190-191 ◽  
pp. 121-132 ◽  
Author(s):  
Byung Il Lee ◽  
You Jung Chung ◽  
Chan Beum Park
Keyword(s):  
Self Assembly ◽  
Β Amyloid

scholarly journals A factor from neurons induces partial immobilization of nonclustered acetylcholine receptors on cultured muscle cells.

1981 ◽  
Vol 88 (2) ◽  
pp. 459-462 ◽  
Author(s):  
D Axelrod ◽  
H C Bauer ◽  
M Stya ◽  
C N Christian
Keyword(s):  
Conditioned Medium ◽  
Acetylcholine Receptors ◽  
Glioma Cell ◽  
Primary Cultures ◽  
Glioma Cell Line ◽  
Continuous Treatment ◽  
Hybrid Cells ◽  
Fluorescence Photobleaching Recovery ◽  
Photobleaching Recovery ◽  
Alpha Bungarotoxin

A factor or factors released by cultured NG108-15 neuroblastoma X glioma hybrid cells and added to the medium of rat myotube primary cultures was found to immobilize some of the previously mobile acetylcholine receptors in the myotube membrane. Partial receptor immobilization occurred within 3 h after the beginning of treatment with the NG108-15-conditioned medium factor and persisted for at least 24 h of continuous treatment. A similarly derived conditioned medium concentrate from the non-neuronal parent glioma cell line did not immobilize receptors, relative to untreated controls. Acetylcholine receptors were visualized by fluorescent alpha-bungarotoxin and their lateral motion was observed by the technique of fluorescence photobleaching recovery.

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