Characterization of Tissue Response and in Vivo Degradation of Cholecyst-Derived Extracellular Matrix

2007 ◽  
Vol 8 (11) ◽  
pp. 3439-3451 ◽  
Author(s):  
Krishna Burugapalli ◽  
Abhay Pandit
Keyword(s):  
Extracellular Matrix ◽  
Tissue Response ◽  
In Vivo Degradation

scholarly journals In vivo degradation of banana starch: Structural characterization of the degradation process

2010 ◽  
Vol 81 (2) ◽  
pp. 291-299 ◽  
Author(s):  
Fernanda H.G. Peroni-Okita ◽  
Renata A. Simão ◽  
Mateus B. Cardoso ◽  
Claudinéia A. Soares ◽  
Franco M. Lajolo ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Degradation Process ◽  
In Vivo Degradation

scholarly journals Modulating in vivo degradation rate of injectable extracellular matrix hydrogels

2016 ◽  
Vol 4 (16) ◽  
pp. 2794-2802 ◽  
Author(s):  
Jean W. Wassenaar ◽  
Rebecca L. Braden ◽  
Kent G. Osborn ◽  
Karen L. Christman
Keyword(s):  
Extracellular Matrix ◽  
Degradation Rate ◽  
In Vivo Degradation

MMP inhibition through doxycycline reduces extracellular matrix (ECM) hydrogel degradation in vivo.

scholarly journals Identification and characterization of a novel extracellular matrix protein nephronectin that is associated with integrin α8β1 in the embryonic kidney

2001 ◽  
Vol 154 (2) ◽  
pp. 447-458 ◽  
Author(s):  
Ralph Brandenberger ◽  
Andrea Schmidt ◽  
James Linton ◽  
Denan Wang ◽  
Carey Backus ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Matrix Protein ◽  
Extracellular Matrix Protein ◽  
Cdna Libraries ◽  
Newborn Mouse ◽  
Metanephric Mesenchyme ◽  
Rgd Sequence ◽  
Kidney Organogenesis

The epithelial–mesenchymal interactions required for kidney organogenesis are disrupted in mice lacking the integrin α8β1. None of this integrin's known ligands, however, appears to account for this phenotype. To identify a more relevant ligand, a soluble integrin α8β1 heterodimer fused to alkaline phosphatase (AP) has been used to probe blots and cDNA libraries. In newborn mouse kidney extracts, α8β1-AP detects a novel ligand of 70–90 kD. This protein, named nephronectin, is an extracellular matrix protein with five EGF-like repeats, a mucin region containing a RGD sequence, and a COOH-terminal MAM domain. Integrin α8β1 and several additional RGD-binding integrins bind nephronectin. Nephronectin mRNA is expressed in the ureteric bud epithelium, whereas α8β1 is expressed in the metanephric mesenchyme. Nephronectin is localized in the extracellular matrix in the same distribution as the ligand detected by α8β1-AP and forms a complex with α8β1 in vivo. Thus, these results strongly suggest that nephronectin is a relevant ligand mediating α8β1 function in the kidney. Nephronectin is expressed at numerous sites outside the kidney, so it may also have wider roles in development. The approaches used here should be generally useful for characterizing the interactions of novel extracellular matrix proteins identified through genomic sequencing projects.

scholarly journals Physicochemical and Tissue Response of PLA Nanofiber Scaffolds Sterilized by Different Techniques

2018 ◽  
pp. 169-180
Author(s):  
Isarai Mendieta-Barrañon DDS ◽  
Osmar A. Chanes-Cuevas DDS, MSc ◽  
Marco A. Álvarez-Pérez PhD ◽  
Patricia González-Alva DDS, PhD ◽  
Luis A. Medina PhD ◽  
...  
Keyword(s):  
Tissue Regeneration ◽  
Physicochemical Characterization ◽  
Biochemical Properties ◽  
Tissue Reaction ◽  
Tissue Response ◽  
Poly Lactic Acid ◽  
Response Analysis ◽  
Sterile Product

In recent years, tissue engineering has evolved considerably, due to the problems in the biomedical area concerning tissue regeneration therapies. Currently, work has been focused on the synthesis and physicochemical characterization of poly lactic acid scaffolds, a synthetic polyester that has been extensively study for its excellent biocompatibility and biodegradability. Moreover, sterilization strategies of scaffold are a crucial step for its application in tissue regeneration, however, the sterilization process have to maintain the structural and biochemical properties of the scaffold. Therefore, it is very important to carry out studies on the sterilization methods of the sample’s material, since translational medicine is intended for in vivo applications. The aim of the present study was designed to analyze the effects of different sterilization techniques, i.e. ethylene oxide (ETO), gamma radiation (GR) and hydrogen peroxide-based plasma (H2O2) in biodegradable PLA scaffolds, and to determine the best sterilization technique to render a sterile product with minimal degradation and deformation, and good tissue response. Analysis of surface morphology showed that ETO and GR modified the PLA scaffolds without any change in its chemical composition. Moreover, the histological response showed that the scaffolds are biocompatible and those sterilized by GR showed a more severe inflammatory response, accompanied with the presence of giant foreign body cells. In conclusion, the results show that among sterilization techniques used in the preset study, the best results were observed with H2O2 sterilization, since it did not significantly modify the surface structure of the PLA fibers and their in vivo response did not cause an unfavorable tissue reaction.

In vivo degradation of magnesium plate/screw osteosynthesis implant systems: Soft and hard tissue response in a calvarial model in miniature pigs

2016 ◽  
Vol 44 (3) ◽  
pp. 309-317 ◽  
Author(s):  
Benoit Schaller ◽  
Nikola Saulacic ◽  
Thomas Imwinkelried ◽  
Stefan Beck ◽  
Edwin Wei Yang Liu ◽  
...  
Keyword(s):  
Tissue Response ◽  
Hard Tissue ◽  
Screw Osteosynthesis ◽  
Miniature Pigs ◽  
In Vivo Degradation

scholarly journals Characterization of Partially Covered Self-Expandable Metallic Stents for Esophageal Cancer Treatment: In Vivo Degradation

2021 ◽  
Vol 7 (4) ◽  
pp. 1403-1413
Author(s):  
Paulina Chytrosz ◽  
Monika Golda-Cepa ◽  
Janusz Wlodarczyk ◽  
Jarosław Kuzdzal ◽  
Miroslawa El Fray ◽  
...  
Keyword(s):  
Esophageal Cancer ◽  
Cancer Treatment ◽  
Metallic Stents ◽  
Expandable Metallic Stents ◽  
In Vivo Degradation

Collagen nanofibril self-assembly on a natural polymeric material for the osteoinduction of stem cells in vitro and biocompatibility in vivo

RSC Advances ◽  
2016 ◽  
Vol 6 (84) ◽  
pp. 80851-80866 ◽  
Author(s):  
A. Aravamudhan ◽  
D. M. Ramos ◽  
N. A. Jenkins ◽  
N. A. Dyment ◽  
M. M. Sanders ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Self Assembly ◽  
Stem Cell Differentiation ◽  
Host Tissue ◽  
Tissue Response ◽  
Microporous Structure

This manuscript reports the characterization of molecularly self-assembled collagen nanofibers on a natural polymeric microporous structure and their ability to support stem cell differentiation in vitro and host tissue response in vivo.

scholarly journals Characterization of BCE-1, a Transcriptional Enhancer Regulated by Prolactin and Extracellular Matrix and Modulated by the State of Histone Acetylation

1998 ◽  
Vol 18 (4) ◽  
pp. 2184-2195 ◽  
Author(s):  
Connie A. Myers ◽  
Christian Schmidhauser ◽  
Julia Mellentin-Michelotti ◽  
Gilberto Fragoso ◽  
Calvin D. Roskelley ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Electrophoretic Mobility ◽  
The Other ◽  
Mobility Shift ◽  
Chromosomal Structure ◽  
Electrophoretic Mobility Shift Analysis ◽  
Shift Analysis ◽  
In Vivo Footprinting

ABSTRACT We have previously described a 160-bp enhancer (BCE-1) in the bovine β-casein gene that is activated in the presence of prolactin and extracellular matrix (ECM). Here we report the characterization of the enhancer by deletion and site-directed mutagenesis, electrophoretic mobility shift analysis, and in vivo footprinting. Two essential regions were identified by analysis of mutant constructions: one binds C/EBP-β and the other binds MGF/STAT5 and an as-yet-unidentified binding protein. However, no qualitative or quantitative differences in the binding of these proteins were observed in electrophoretic mobility shift analysis using nuclear extracts derived from cells cultured in the presence or absence of ECM with or without prolactin, indicating that prolactin- and ECM-induced transcription was not dependent on the availability of these factors in the functional cell lines employed. An in vivo footprinting analysis of the factors bound to nuclear chromatin in the presence or absence of ECM and/or prolactin found no differences in the binding of C/EBP-β but did not provide definitive results for the other factors. Neither ECM nor prolactin activated BCE-1 in transient transfections, suggesting that the chromosomal structure of the integrated template may be required for ECM-induced transcription. Further evidence is that treatment of cells with inhibitors of histone deacetylase was sufficient to induce transcription of integrated BCE-1 in the absence of ECM. Together, these results suggest that the ECM induces a complex interaction between the enhancer-bound transcription factors, the basal transcriptional machinery, and a chromosomally integrated template responsive to the acetylation state of the histones.

Study of biodegradation and biocompatibility of PEGylated rosin derivatives

2017 ◽  
Vol 32 (6) ◽  
pp. 628-640 ◽  
Author(s):  
Dinesh M Morkhade ◽  
Vishwanath S Nande ◽  
Umesh V Barabde ◽  
Siddheshwar B Joshi
Keyword(s):  
Molecular Weight ◽  
Weight Loss ◽  
Polyethylene Glycol ◽  
Inflammatory Responses ◽  
Tissue Response ◽  
Polyethylene Glycol 400 ◽  
Constant Weight ◽  
In Vivo Degradation

PEGylated rosin derivatives are improved in series ester-adduct derivatives of rosin. The aim of this study was to assess biodegradation and biocompatibility of PEGylated rosin derivatives. Study employed two different PEGylated rosin derivatives, namely, D1 and D2, with constant weight of rosin and increasing amounts of polyethylene glycol 400. PEGylated rosin derivatives were synthesized and tailored into spherical beads and disks with smooth surface for use. In vitro degradation was studied at pH 4.0, 7.4, and 10 for 60 days. In vivo study was performed in Wistar rats using poly(d,l-lactide- co-glycolide) (50:50) as a control. Post 3, 7, 14, and 21 days of implantation, PEGylated rosin derivatives disks were retrieved and evaluated for weight loss, molecular weight decline, morphology, and tissue response. D1 and D2 beads showed 21.68% and 32.37% weight loss, respectively, at pH 7.4 post 60 days. Degradation was increased substantially with increase in pH of medium. Degradation of disks was markedly slower than that of beads. In vivo degradation of PEGylated rosin derivative disks was faster than in vitro. Post 60 days of implantation, weight loss of D1 and D2 disk was 7.57% and 11.84%, whereas molecular weight was declined by about 19% and 26%, respectively. Owing to higher amounts of polyethylene glycol 400, in vitro and in vivo degradation of D2 was faster than D1. Poly(d,l-lactide- co-glycolide) as well as PEGylated rosin derivative implants evoked mild inflammatory responses characterized by few macrophages and absence of exudation at tissue–disk interface. The cellular density in tissue surrounding PEGylated rosin derivative disks increased initially with time up to 7 days and decreased eventually at the end of 21 days. The trend was similar for poly(d,l-lactide- co-glycolide) implants. Increase in polyethylene glycol 400 improved biodegradation and biocompatibility of PEGylated rosin derivatives. Results revealed that PEGylated rosin derivatives degrade slowly in vivo over a period of time, possess fair biocompatibility, and thus are promising biomaterial for drug delivery applications.

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