scholarly journals Stable, Metastable, and Kinetically Trapped Amyloid Aggregate Phases

2014 ◽  
Vol 16 (1) ◽  
pp. 326-335 ◽  
Author(s):  
Tatiana Miti ◽  
Mentor Mulaj ◽  
Jeremy D. Schmit ◽  
Martin Muschol
Keyword(s):  
Amyloid Aggregate

scholarly journals Bifunctional fluorescent probes for detection of amyloid aggregates and reactive oxygen species

2018 ◽  
Vol 5 (2) ◽  
pp. 171399 ◽  
Author(s):  
Lisa-Maria Needham ◽  
Judith Weber ◽  
James W. B. Fyfe ◽  
Omaru M. Kabia ◽  
Dung T. Do ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Neurodegenerative Disorders ◽  
Oxygen Species ◽  
Fluorescence Measurements ◽  
Amyloid Aggregate ◽  
Amyloid Deposits ◽  
Amyloid Aggregates ◽  
Highly Sensitive ◽  
Key Features ◽  
And Oxidative Stress

Protein aggregation into amyloid deposits and oxidative stress are key features of many neurodegenerative disorders including Parkinson's and Alzheimer's disease. We report here the creation of four highly sensitive bifunctional fluorescent probes, capable of H 2 O 2 and/or amyloid aggregate detection. These bifunctional sensors use a benzothiazole core for amyloid localization and boronic ester oxidation to specifically detect H 2 O 2 . We characterized the optical properties of these probes using both bulk fluorescence measurements and single-aggregate fluorescence imaging, and quantify changes in their fluorescence properties upon addition of amyloid aggregates of α-synuclein and pathophysiological H 2 O 2 concentrations. Our results indicate these new probes will be useful to detect and monitor neurodegenerative disease.

scholarly journals Estimation of amyloid aggregate sizes with semi-denaturing detergent agarose gel electrophoresis and its limitations

Prion ◽  
2020 ◽  
Vol 14 (1) ◽  
pp. 118-128 ◽  
Author(s):  
Polina B. Drozdova ◽  
Yury A. Barbitoff ◽  
Mikhail V. Belousov ◽  
Rostislav K. Skitchenko ◽  
Tatyana M. Rogoza ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Amyloid Aggregate

Piezoelectric materials for ultrasound-driven dissociation of Alzheimer's β-amyloid aggregate structure

Biomaterials ◽  
2020 ◽  
Vol 255 ◽  
pp. 120165 ◽  
Author(s):  
Jinhyeong Jang ◽  
Kayoung Kim ◽  
Jaeho Yoon ◽  
Chan Beum Park
Keyword(s):  
Piezoelectric Materials ◽  
Aggregate Structure ◽  
Amyloid Aggregate ◽  
Β Amyloid

PEG modified graphene oxide loaded with EALYLV peptides for inhibiting the aggregation of hIAPP associated with type-2 diabetes

2015 ◽  
Vol 3 (35) ◽  
pp. 7055-7067 ◽  
Author(s):  
Xianbo Zhou ◽  
Chengwen Cao ◽  
Qingchang Chen ◽  
Qianqian Yu ◽  
Yanan Liu ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Graphene Oxide ◽  
Pancreatic Islets ◽  
Islet Amyloid Polypeptide ◽  
Human Islet ◽  
Islet Amyloid ◽  
Amyloid Aggregate ◽  
Modified Graphene Oxide ◽  
Modified Graphene

Human islet amyloid polypeptide (hIAPP) was found as amyloid aggregate deposits in the pancreatic islets of patients with type-2 diabetes and studies showed that insulin and its derivatives were the potent inhibitors of hIAPP aggregation.

scholarly journals Targeting Alzheimer’s disease with multimodal polypeptide-based nanoconjugates

2021 ◽  
Vol 7 (13) ◽  
pp. eabf9180
Author(s):  
A. Duro-Castano ◽  
C. Borrás ◽  
V. Herranz-Pérez ◽  
M. C. Blanco-Gandía ◽  
I. Conejos-Sánchez ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Pyramidal Neurons ◽  
Brain Distribution ◽  
Olfactory Memory ◽  
Hippocampal Culture ◽  
Amyloid Aggregate ◽  
Organotypic Hippocampal Culture ◽  
Β Amyloid ◽  
Prevalent Form

Alzheimer’s disease (AD), the most prevalent form of dementia, remains incurable mainly due to our failings in the search for effective pharmacological strategies. Here, we describe the development of targeted multimodal polypeptide-based nanoconjugates as potential AD treatments. Treatment with polypeptide nanoconjugates bearing propargylamine moieties and bisdemethoxycurcumin or genistein afforded neuroprotection and displayed neurotrophic effects, as evidenced by an increase in dendritic density of pyramidal neurons in organotypic hippocampal culture. The additional conjugation of the Angiopep-2 targeting moiety enhanced nanoconjugate passage through the blood-brain barrier and modulated brain distribution with nanoconjugate accumulation in neurogenic areas, including the olfactory bulb. Nanoconjugate treatment effectively reduced neurotoxic β amyloid aggregate levels and rescued impairments to olfactory memory and object recognition in APP/PS1 transgenic AD model mice. Overall, this study provides a description of a targeted multimodal polyglutamate-based nanoconjugate with neuroprotective and neurotrophic potential for AD treatment.

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