Hyaluronic Acid-Based 3D Culture Model for In Vitro Testing of Electrode Biocompatibility

2014 ◽  
Vol 15 (6) ◽  
pp. 2157-2165 ◽  
Author(s):  
Andrea F. Jeffery ◽  
Matthew A. Churchward ◽  
Vivian K. Mushahwar ◽  
Kathryn G. Todd ◽  
Anastasia L. Elias
Keyword(s):  
Hyaluronic Acid ◽  
3D Culture ◽  
In Vitro Testing ◽  
Culture Model ◽  
3D Culture Model

scholarly journals Use of hydrogel scaffolds to develop an in vitro 3D culture model of human intestinal epithelium

2017 ◽  
Vol 62 ◽  
pp. 128-143 ◽  
Author(s):  
R.H. Dosh ◽  
A. Essa ◽  
N. Jordan-Mahy ◽  
C. Sammon ◽  
C.L. Le Maitre
Keyword(s):  
Intestinal Epithelium ◽  
3D Culture ◽  
Hydrogel Scaffolds ◽  
Culture Model ◽  
3D Culture Model ◽  
Human Intestinal Epithelium

The Anti-Angiogenic Effect of Atorvastatin Loaded Exosomes on Glioblastoma Tumor Cells: An in Vitro 3D Culture Model

2022 ◽  
Author(s):  
Faezeh Esmaeli Ranjbar ◽  
Elahe valipour ◽  
Ziba Veisi Malekshahi ◽  
Neda Mokhberian ◽  
Zahra Taghdiri-Nooshabadi ◽  
...  
Keyword(s):  
Tumor Cells ◽  
3D Culture ◽  
Culture Model ◽  
Angiogenic Effect ◽  
3D Culture Model

scholarly journals Study on a 3D Hydrogel-Based Culture Model for Characterizing Growth of Fibroblasts under Viral Infection and Drug Treatment

2017 ◽  
Vol 22 (5) ◽  
pp. 626-634 ◽  
Author(s):  
Xiaolu Zhu ◽  
Xianting Ding
Keyword(s):  
Morphological Evolution ◽  
Three Dimensional ◽  
3D Culture ◽  
Control Group ◽  
Herpes Simplex Virus 1 ◽  
3T3 Fibroblasts ◽  
Culture Model ◽  
3D Culture Model ◽  
Hsv 1

Three-dimensional (3D) in vitro tissue models provide an approach for the systematic, repetitive, and quantitative study of drugs. In this study, we constructed an in vitro 3D acrylated hyaluronic acid (AHA) hydrogel model encapsulating fibroblasts, performed long-period 3D culture, and tested cellular topological changes and proliferation variation in the presence of herpes simplex virus-1 (HSV-1) as an infecting virus and acyclovir (ACV) as the treatment drug. The AHA hydrogels were formed by using Michael addition chemistry of bis-cysteine containing MMP-degradable cross-linker onto AHA prefunctionalized with cell adhesion peptides (RGD). Cellular structures of 3T3 fibroblasts in hydrogel presented different morphological evolution processes and proliferation rates between different groups, including HSV-1 treated alone, ACV treated alone, HSV-1 and ACV cotreated, and control samples. In AHA hydrogel, ACV blocked HSV-1 infection/replication on fibroblasts. Yet, the proliferation of ACV-treated fibroblasts was slower than that of the control group. A significantly longer period was required for cells in 3D AHA gel to regain a healthy status when compared with cells in two-dimensional (2D) culture. This hydrogel-based 3D culture model potentially lays a foundation for analyzing the response of self-organized 3D tissues to viruses and drugs in a way that is closer to nature.

scholarly journals 3D Bioprinting Allows the Establishment of Long-Term 3D Culture Model for Chronic Lymphocytic Leukemia Cells

2021 ◽  
Vol 12 ◽  
Author(s):  
Francesca Vittoria Sbrana ◽  
Riccardo Pinos ◽  
Federica Barbaglio ◽  
Davide Ribezzi ◽  
Fiorella Scagnoli ◽  
...  
Keyword(s):  
Gene Expression ◽  
3D Culture ◽  
Lymphocytic Leukemia ◽  
Leukemic Cells ◽  
3D Bioprinting ◽  
Culture Model ◽  
3D Culture Model

Chronic Lymphocytic Leukemia (CLL) represents the most common leukemia in the western world and remains incurable. Leukemic cells organize and interact in the lymphoid tissues, however what actually occurs in these sites has not been fully elucidated yet. Studying primary CLL cells in vitro is very challenging due to their short survival in culture and also to the fact that traditional two-dimensional in vitro models lack cellular and spatial complexity present in vivo. Based on these considerations, we exploited for the first time three-dimensional (3D) bioprinting to advance in vitro models for CLL. This technology allowed us to print CLL cells (both primary cells and cell lines) mixed with the appropriate, deeply characterized, hydrogel to generate a scaffold containing the cells, thus avoiding the direct cell seeding onto a precast 3D scaffold and paving the way to more complex models. Using this system, we were able to efficiently 3D bioprint leukemic cells and improve their viability in vitro that could be maintained up to 28 days. We monitored over time CLL cells viability, phenotype and gene expression, thus establishing a reproducible long-term 3D culture model for leukemia. Through RNA sequencing (RNAseq) analysis, we observed a consistent difference in gene expression profile between 2D and 3D samples, indicating a different behavior of the cells in the two different culture settings. In particular, we identified pathways upregulated in 3D, at both day 7 and 14, associated with immunoglobulins production, pro-inflammatory molecules expression, activation of cytokines/chemokines and cell-cell adhesion pathways, paralleled by a decreased production of proteins involved in DNA replication and cell division, suggesting a strong adaptation of the cells in the 3D culture. Thanks to this innovative approach, we developed a new tool that may help to better mimic the physiological 3D in vivo settings of leukemic cells as well as of immune cells in broader terms. This will allow for a more reliable study of the molecular and cellular interactions occurring in normal and neoplastic conditions in vivo, and could also be exploited for clinical purposes to test individual responses to different drugs.

Combination of proteasome inhibitors with temozolomide to increase the anti-tumor effect in 3D culture model of glioblastoma

2018 ◽  
Vol 120 ◽  
pp. S152
Author(s):  
Semra Unal ◽  
Tilbe Gokce ◽  
Sema Arslan ◽  
Ayse Mine Yilmaz ◽  
Oguzhan Gunduz ◽  
...  
Keyword(s):  
Proteasome Inhibitors ◽  
3D Culture ◽  
Culture Model ◽  
3D Culture Model

Methyl-β-cyclodextrin treatment combined to incubation with interleukin-4 reproduces major features of atopic dermatitis in a 3D-culture model

2016 ◽  
Vol 309 (1) ◽  
pp. 63-69 ◽  
Author(s):  
Tatiana do Nascimento Pedrosa ◽  
Evelyne De Vuyst ◽  
Abdallah Mound ◽  
Catherine Lambert de Rouvroit ◽  
Silvya Stuchi Maria-Engler ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
3D Culture ◽  
Interleukin 4 ◽  
Culture Model ◽  
3D Culture Model
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