Thermoresponsive Copolymer Brushes Possessing Quaternary Amine Groups for Strong Anion-Exchange Chromatographic Matrices

2014 ◽  
Vol 15 (3) ◽  
pp. 1031-1043 ◽  
Author(s):  
Kenichi Nagase ◽  
Mike Geven ◽  
Saori Kimura ◽  
Jun Kobayashi ◽  
Akihiko Kikuchi ◽  
...  
Keyword(s):  
Anion Exchange ◽  
Quaternary Amine ◽  
Strong Anion Exchange ◽  
Amine Groups ◽  
Copolymer Brushes

scholarly journals Perception on aggregation induced multicolor emission and emission centers in carbon nanodots using successive dilution, anion exchange chromatography, and multi-way statistics

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Mohsen Kompany-Zareh ◽  
Saeed Bagheri
Keyword(s):  
Anion Exchange ◽  
Quantum Confinement Effect ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Carbon Nanodots ◽  
Surface Charges ◽  
Negative Surface ◽  
Amine Groups ◽  
Optical Behavior ◽  
Optical Structure

AbstractExploration in the way of understanding the optical behavior and structure of carbon nanodots has been increased due to their vast application. Their emission dependency on excitation wavelengths is the more prevalent and controversial subject. In this report we considered the optical structure of hydrothermally synthesized carbon nanodots using citric acid and 2,3-diaminopyridine as precursors. The presence of different emission centers experimented through anion exchange chromatography which resulted in fractions with more unique optical structures. The quantum confinement effect and energy exchange between different types of carbon nanodots, due to aggregation in higher concentration levels, was studied applying a stepwise dilution experiment. Analysis of the experimental data was done through the parallel factor analysis and the trajectory pattern recognition which resolved more about optical interactions and the presence of different emission centers in different particles. Results from infrared spectroscopy confirmed the dominating density of carboxyl functional groups on the nanodots with negative surface charges and higher influence of amine groups on dots with positive surface charges.

scholarly journals Extensive non-canonical phosphorylation in human cells revealed using strong-anion exchange-mediated phosphoproteomics

2017 ◽  
Author(s):  
Gemma Hardman ◽  
Simon Perkins ◽  
Zheng Ruan ◽  
Natarajan Kannan ◽  
Philip Brownridge ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Protein Phosphorylation ◽  
Anion Exchange ◽  
Human Cell ◽  
Cell Biology ◽  
Human Cells ◽  
Phosphopeptide Enrichment ◽  
Cell Extracts ◽  
Strong Anion Exchange ◽  
Acid Labile

Protein phosphorylation is a ubiquitous post-translational modification (PTM) that regulates all aspects of life. To date, investigation of human cell signalling has focussed on canonical phosphorylation of serine (Ser), threonine (Thr) and tyrosine (Tyr) residues. However, mounting evidence suggests that phosphorylation of histidine also plays a central role in regulating cell biology. Phosphoproteomics workflows rely on acidic conditions for phosphopeptide enrichment, which are incompatible with the analysis of acid-labile phosphorylation such as histidine. Consequently, the extent of non-canonical phosphorylation is likely to be under-estimated. We report an Unbiased Phosphopeptide enrichment strategy based on Strong Anion Exchange (SAX) chromatography (UPAX), which permits enrichment of acid-labile phosphopeptides for characterisation by mass spectrometry. Using this approach, we identify extensive and positional phosphorylation patterns on histidine, arginine, lysine, aspartate and glutamate in human cell extracts, including 310 phosphohistidine and >1000 phospholysine sites of protein modification. Remarkably, the extent of phosphorylation on individual non-canonical residues vastly exceeds that of basal phosphotyrosine. Our study reveals the previously unappreciated diversity of protein phosphorylation in human cells, and opens up avenues for exploring roles of acid-labile phosphorylation in any proteome using mass spectrometry.

scholarly journals Efficiency of crude corn extract clean-up on different columns in fumonisins determination

2005 ◽  
pp. 95-102 ◽  
Author(s):  
Biljana Abramovic ◽  
Sandra Jaksic ◽  
Zoran Masic
Keyword(s):  
Liquid Chromatography ◽  
Anion Exchange ◽  
Fluorescence Detection ◽  
Crude Extract ◽  
Reversed Phase ◽  
Lower Price ◽  
Strong Anion Exchange ◽  
Chromatographic Resolution ◽  
Immunoaffinity Columns ◽  
Fumonisins B1 And B2

The efficiencies of different clean-up procedures for crude corn extract from corn samples naturally contaminated by fumonisins B1 and B2 were compared. These procedures precede liquid chromatography determination with fluorescence detection. The efficiencies of immunoaffinity columns (IMA) strong anion exchange columns (SAX), as well as columns with reversed-phase C18 (RP C18) were investigated. No significant differences in the obtained results were found, regardless of the crude extract clea-nup procedure. However, the use of IMA columns for clean-up provided better chromatographic resolution, with the clean-up procedure being the simplest and the fastest. Also, because of the possibility of IMA column regeneration, it is possible to prepare ten samples on one column, so all in all, the lower price of SAX and RP C18 columns is of no great significance.

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