Clearance Kinetics of Biomaterials Affects Stem Cell Retention and Therapeutic Efficacy

2014 ◽  
Vol 15 (2) ◽  
pp. 564-573 ◽  
Author(s):  
Chia Y. Lai ◽  
Pei J. Wu ◽  
Steve R. Roffler ◽  
Sho T. Lee ◽  
Shiaw M. Hwang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Therapeutic Efficacy ◽  
Cell Retention ◽  
Clearance Kinetics ◽  
Kinetics Of

scholarly journals Synergistic Effects of N-Acetylcysteine and Mesenchymal Stem Cell in a Lipopolysaccharide-Induced Interstitial Cystitis Rat Model

Cells ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 86 ◽  
Author(s):  
Jung Hyun Shin ◽  
Chae-Min Ryu ◽  
Hyein Ju ◽  
Hwan Yeul Yu ◽  
Sujin Song ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Interstitial Cystitis ◽  
Rat Model ◽  
Therapeutic Efficacy ◽  
Synergistic Effects ◽  
Embryonic Stem ◽  
Protamine Sulfate ◽  
Sprague Dawley ◽  
Multipotent Mesenchymal Stem Cells

The purpose of this study was to reduce the amount of stem cells used in treating preclinical interstitial cystitis (IC model) by investigating the synergistic effects of multipotent mesenchymal stem cells (M-MSCs; human embryonic stem cell-derived) and N-acetylcysteine (NAC). Eight-week-old female Sprague-Dawley rats were divided into seven groups, i.e., sham (n = 10), lipopolysaccharide/protamine sulfate (LPS/PS; n = 10), LPS/PS + NAC (n = 10), LPS/PS with 25K MSC (n = 10), LPS/PS with 50K MSC (n = 10) LPS/PS + 25K MSC + NAC (n = 10), and LPS/PS + 50K MSC + NAC (n = 10). To induce the IC rat model, protamine sulfate (10 mg, 45 min) and LPS (750 μg, 30 min) were instilled once a week for five consecutive weeks via a transurethral PE-50 catheter. Phosphate-buffered saline (PBS) was used in the sham group. One week after the final instillation, M-MSCs with two suboptimal dosages (i.e., 2.5 or 5.0 × 104 cells) were directly transplanted into the outer-layer of the bladder. Simultaneously, 200 mg/kg of NAC or PBS was intraperitoneally injected daily for five days. The therapeutic outcome was evaluated one week after M-MSC or PBS injection by awake cystometry and histological analysis. Functionally, LPS/PS insult led to irregular micturition, decreased intercontraction intervals, and decreased micturition volume. Both monotherapy and combination therapy significantly increased contraction intervals, increased urination volume, and reduced the residual volume, thereby improving the urination parameters compared to those of the LPS group. In particular, a combination of NAC dramatically reduced the amount of M-MSCs used for significant restoration in histological damage, including inflammation and apoptosis. Both M-MSCs and NAC-based therapy had a beneficial effect on improving voiding dysfunction, regenerating denudated urothelium, and relieving tissue inflammation in the LPS-induced IC/BPS rat model. The combination of M-MSC and NAC was superior to MSC or NAC monotherapy, with therapeutic efficacy that was comparable to that of previously optimized cell dosage (1000K) without compromised therapeutic efficacy.

scholarly journals Biological Activities Encoded by the Murine Mesenchymal Stem Cell Transcriptome Provide a Basis for Their Developmental Potential and Broad Therapeutic Efficacy

Stem Cells ◽  
2006 ◽  
Vol 24 (1) ◽  
pp. 186-198 ◽  
Author(s):  
Donald G. Phinney ◽  
Katy Hill ◽  
Charles Michelson ◽  
Maria DuTreil ◽  
Catherine Hughes ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Therapeutic Efficacy ◽  
Biological Activities ◽  
Developmental Potential ◽  
Cell Transcriptome

Kinetics of peripheral blood stem cell harvests during a single apheresis

Transfusion ◽  
1999 ◽  
Vol 39 (4) ◽  
pp. 403-409 ◽  
Author(s):  
Adam G. Smolowicz ◽  
Kenneth Villman ◽  
Gösta Berlin ◽  
Ulf Tidefelt
Keyword(s):  
Stem Cell ◽  
Peripheral Blood ◽  
Peripheral Blood Stem Cell ◽  
Blood Stem Cell ◽  
Kinetics Of

Comparative Clearance Kinetics of Beat Damaged (Hdrc) and Antibody Coated Red Cells (Igrc)

1982 ◽  
Vol 62 (2) ◽  
pp. 7P-7P ◽  
Author(s):  
A.M. Peters ◽  
M. J. Walport ◽  
K. B. Elkon ◽  
P. P. Ferjencik ◽  
G. R. V. Hughes ◽  
...  
Keyword(s):  
Red Cells ◽  
Clearance Kinetics ◽  
Kinetics Of

The lung clearance kinetics of 57Co3O4 in rats of various ages

1991 ◽  
Vol 22 (4) ◽  
pp. 537-549 ◽  
Author(s):  
C.G. Collier ◽  
A. Hodgson ◽  
S.A. Gray ◽  
J.C. Moody ◽  
A. Ball
Keyword(s):  
Lung Clearance ◽  
Clearance Kinetics ◽  
Kinetics Of

IgA nephropathy: clearance kinetics of IgA-containing immune complexes

2018 ◽  
Vol 40 (6) ◽  
pp. 539-543 ◽  
Author(s):  
Ann Chen ◽  
Sung-Sen Yang ◽  
Tsai-Jung Lin ◽  
Shuk-Man Ka
Keyword(s):  
Iga Nephropathy ◽  
Immune Complexes ◽  
Clearance Kinetics ◽  
Kinetics Of

scholarly journals Nidogen-1 Contributes to the Interaction Network Involved in Pro-B Cell Retention in the Peri-sinusoidal Hematopoietic Stem Cell Niche

Cell Reports ◽  
2019 ◽  
Vol 26 (12) ◽  
pp. 3257-3271.e8 ◽  
Author(s):  
Marielle Balzano ◽  
Maria De Grandis ◽  
Thien-Phong Vu Manh ◽  
Lionel Chasson ◽  
Florence Bardin ◽  
...  
Keyword(s):  
Stem Cell ◽  
B Cell ◽  
Hematopoietic Stem Cell ◽  
Stem Cell Niche ◽  
Interaction Network ◽  
Cell Retention ◽  
Hematopoietic Stem ◽  
Hematopoietic Stem Cell Niche ◽  
Cell Niche ◽  
Nidogen 1
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