Surface Engineering for Long-Term Culturing of Mesenchymal Stem Cell Microarrays

2013 ◽  
Vol 14 (8) ◽  
pp. 2675-2683 ◽  
Author(s):  
Soraya Rasi Ghaemi ◽  
Frances Harding ◽  
Bahman Delalat ◽  
Roshan Vasani ◽  
Nicolas H. Voelcker
Nanoscale ◽  
2020 ◽  
Author(s):  
Naishun Liao ◽  
Da Zhang ◽  
Ming Wu ◽  
Huang-Hao Yang ◽  
Xiaolong Liu ◽  
...  

Adipose tissue derived mesenchymal stem cell (ADSC)-based therapy is attractive for liver diseases, but the long-term therapeutic outcome is still far from satisfaction due to low hepatic engraftment efficiency of...


2014 ◽  
Vol 44 (12) ◽  
pp. 1546-1557 ◽  
Author(s):  
J. E. Trzil ◽  
I. Masseau ◽  
T. L. Webb ◽  
C.-H. Chang ◽  
J. R. Dodam ◽  
...  

2015 ◽  
Vol 3 (2) ◽  
pp. 383-390 ◽  
Author(s):  
Yon Jin Chuah ◽  
Shreyas Kuddannaya ◽  
Min Hui Adeline Lee ◽  
Yilei Zhang ◽  
Yuejun Kang

Surface silanization with 3-aminopropyl triethoxy silane (APTES) ± glutaraldehyde (GA) enhanced the biocompatibility of poly(dimethylsiloxane) surfaces for long term cell culture investigation.


2019 ◽  
Vol 7 (8) ◽  
pp. 1252-1258 ◽  
Author(s):  
Vivi Sofia ◽  
Moch Saiful Bachri ◽  
Rizki Rahmadian

BACKGROUND: Pharmacological therapy in the management of OA causes many new health problems due to side effects caused by long-term use of drugs, such as long-term use of Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) will cause gastric ulcers and impaired kidney function. In OA pathogenesis, PGE2 gene is involved in the inflammation process. AIM: This study aims to identify the influence of Wharton Jelly Mesenchymal Stem Cell (MSC-WJ) on PGE2 expression gene in synoviocyte by in vitro. MATERIAL AND METHODS: The method used in this study is the co-culture method of primary cells and stem cells in the appropriate media. This research is pure experimental research. The sample used came from synovial tissue of osteoarthritis patients who underwent Total Knee Replacement (TKR) surgery. This study was divided into 6 groups treated with 4 replications. The expression analysis of the Prostaglandin E2 gene was done using qPCR (Real-Time Polymerase Chain Reaction). The expression analysis of the Prostaglandin E2 gene was carried out before and after the co-culture with Wharton's Jelly and continued with the analysis of statistical data processing using the SPSS.15 program. PGE2 gene expression data were processed using the Kruskal-Wallis test and continued with the Mann-Whitney test with a 95% confidence level. RESULTS: The results showed that Mesenchymal Stem Cells Wharton Jelly could reduce the expression of Prostaglandin E2 gene after co-culture for 24 hours and 48 hours in synoviocyte cells osteoarthritis significantly compared with the control group. The administration of Mesenchymal Stem Cells for 24 hours reduced the expression level of PGE2 gene by 0.61 times compared to the control group (p < 0.05) and the administration of Mesenchymal Stem Cells for 48 hours decreased the expression level of PGE2 gene by 0, 47 times compared to the control group (p < 0.05). CONCLUSION: This study concluded that MSC-WJ in OA synoviocyte significantly reduced the expression of the PGE2 gene (p < 0.05).


2019 ◽  
Vol 83 (7) ◽  
pp. 1590-1599 ◽  
Author(s):  
Takeshi Yagyu ◽  
Satoshi Yasuda ◽  
Noritoshi Nagaya ◽  
Kaori Doi ◽  
Takeshi Nakatani ◽  
...  

2016 ◽  
Vol 2 (5) ◽  
pp. 055016 ◽  
Author(s):  
Sanjukta Guha Thakurta ◽  
Neety Sahu ◽  
April Miller ◽  
Gaurav Budhiraja ◽  
Leonard Akert ◽  
...  

PLoS ONE ◽  
2013 ◽  
Vol 8 (1) ◽  
pp. e51253 ◽  
Author(s):  
Vanessa Donega ◽  
Cindy T. J. van Velthoven ◽  
Cora H. Nijboer ◽  
Frank van Bel ◽  
Martien J. H. Kas ◽  
...  

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